>> I have heard diametrically opposed statements about the
effectiveness of
> standard steam autoclaves in terms of destroying DNA (on microtubes,
forceps,
> pestles, etc.) such that PCR will not amplify any DNA that may have been
on
> the autoclaved items. One group says that 15-20 min in the autoclave at
121C
YES, autoclaving will destroy DNA. To be sure, autoclave small batches for
30-45 minutes. 121C is beyond the melting point of any DNA.
