Francois Jeanmougin wrote:
>> In article <01bb83e4$58093080$47faa4c6 at skinner.mi.net>,
> "Barn" <skinner at mis.nb.ca> writes:
>> >121C
> >YES, autoclaving will destroy DNA. To be sure, autoclave small batches for
> >30-45 minutes. 121C is beyond the melting point of any DNA.
> >
>> Well, don't confuse between denaturation and destruction. the
> destruction should come from covalent bond hydrolysis. Then the buffer
> in which you are is important, at least its boiling point and the presence
> of hydrolytic agents.
>> Hope this helps,
>
There are exceptions... If you are really paranoid, there are bacteria
that can handle such temperatures. I don't have a source though.
Chris
