Hello,
I'm engaged in a project of phylogeny using cyt-b gene. I'd like to hear
opinions about the best way of amplifying mt-DNA. I see that most people
amplify cyt-b from total DNA, but others isolate the mt-DNA previously in order
to avoid nuclear interferences.
What do you think about this?
Thanks in advance.
Claudio Slamovits
University of Buenos Aires
