> I'm engaged in a project of phylogeny using cyt-b gene. I'd like to hear
>opinions about the best way of amplifying mt-DNA. I see that most people
>amplify cyt-b from total DNA, but others isolate the mt-DNA previously in order
>to avoid nuclear interferences.
>What do you think about this?
Just proteinase digest the tissue for 1-2 hrs. and phenol-extract
it and precipitate the DNA with EtOH. Or use kits such as DNA-isolator
or Direct DNA (biotin). Nothing to it!