Restriction Enzyme Phylogenies

Mark Siddall mes at zoo.toronto.edu
Tue Feb 13 22:49:43 EST 1996

In article <4fpfsm$q3f at newsbf02.news.aol.com> daro at aol.com (Daro) writes:
>It seems most phylogenies inferred from RE data assay the entire
>chromosome.  For instance, I have seen several studies where restriction
>fragments are P32 labeled and then analyzed for various "phenotypes". 
>However, my research involves a more limited assay...I use an
>oligonucleotide probe for a specific gene instead of labeling all the
>fragments.  Can I still calculate genetic distance (for example, Nei and
>Li's method) from this data or would this distance reflect the specific
>gene's (i.e. probed gene) distances?
>Ashley,daro at aol.com

In my all-too-typical nay-saying fashion, perhaps you should contemplate
whether or not these data are reasonable for phylogenetic inference.
I should bother you that radically different melting curves
could have identical T50 values for starters.

Mark E. Siddall                "I don't mind a parasite...
mes at vims.edu                    I object to a cut-rate one" 
Virginia Inst. Marine Sci.                     - Rick
Gloucester Point, VA, 23062

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