Hi, there, I have trouble in DNA ligation, the case below:
Insert Fragment: 16.3kb SalI Frag.
Vector: Bscrip-KS(-) SalI cut plasmid or other
salI cut expression vector
It looks like no ligation problem, but in fact, I cannot
success to ligate them and transfer them into DH5a no matter I use
chemical or electroparation method, Have anybody ever meet the same problem?
How can I solve them? Any suggestion is appreciated! Thanks for you attention!