Yves,
I worked on Clostridia for a while, sequencing this region. I found that this
is a hyper-variable region, with taxa from the same 'species' having
polymorphisms in this region. Most of these polymorphisms were in the form of
base substitutions, but there were insertion-deletion events also.
I couldn't publish the results due to confidentiality agreements and other
such crap.
Hope this helps a little,
James
Yves Bertheau wrote:
>> Hello,
>> We are working on phytopathogenic enterobacteriaceae: the
> pectinolytic erwinias and presently particularly on the pathovars
> of Erwinia chrysanthemi.
>> We observe a size polymorphism, when we amplify by PCR the ITS
> located between the 16 and 23S subunits of the rRNA.
>> Does anybody know references about such a polymorphism between
> species but also more particularly between strains of a species?
> Has such a polymorphism been correlated to infra-species divisions?
>> Thanking you in advance for your help.
>> Sincerely
>> Yves Bertheau
> Pathologie vegetale
> INRA, Paris, FRANCE
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James O. McInerney email: J.mcinerney at nhm.ac.uk
Molec. Biol. Comput. Officer, phone: +44 171 938 9247
Department of Zoology, Fax: +44 171 938 9158
The Natural History Museum,
Cromwell Road,
London SW7 5BD.
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