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ITS polymorphism and taxonomy/phylogeny

James McInerney jamm at nhm.ac.uk
Thu Dec 4 10:27:25 EST 1997


I worked on Clostridia for a while, sequencing this region.  I found that this
is a hyper-variable region, with taxa from the same 'species' having
polymorphisms in this region.  Most of these polymorphisms were in the form of
base substitutions, but there were insertion-deletion events also.

I couldn't publish the results due to confidentiality agreements and other
such crap.

Hope this helps a little,


Yves Bertheau wrote:
> Hello,
> We are working on phytopathogenic enterobacteriaceae: the
> pectinolytic erwinias and presently particularly on the pathovars
> of Erwinia chrysanthemi.
> We observe a size polymorphism, when we amplify by PCR the ITS
> located between the 16 and 23S subunits of the rRNA.
> Does anybody know references about such a polymorphism between
> species but also more particularly between strains of a species?
> Has such a polymorphism been correlated to infra-species divisions?
> Thanking you in advance for your help.
> Sincerely
>                 Yves Bertheau
>                 Pathologie vegetale
>                 INRA, Paris, FRANCE

James O. McInerney               email: J.mcinerney at nhm.ac.uk
Molec. Biol. Comput. Officer,    phone: +44 171 938 9247
Department of Zoology,           Fax:   +44 171 938 9158
The Natural History Museum,
Cromwell Road,                    
London SW7 5BD.                  

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