Hello Fabio -
You don't mention what locus you are trying to amplify, but ...
See my workshop tutorial on exactly this topic (if you have access to
GCG software): http://bio.fsu.edu/~stevet/Primer_Design.pdf
Even without GCG you could still use the principles outlined there.
Cheers - Steve
Steven M. Thompson
A C T G stevet at bio.fsu.edu
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