From almaldon from gmail.com  Tue Jun 23 06:43:52 2009
From: almaldon from gmail.com (Ana Maldonado)
Date: Tue Jun 23 08:46:13 2009
Subject: [Molecular-evolution] Palindrome and non-palindrome
Message-ID: <1870db470906230443q4484dcbufb8cd73ace225cd5@mail.gmail.com>

Dear Dr. Gegenheimer,
I hope this e-mail finds you well.

I sought that you gave some reasons to explain why palindromic recognition
sequences.
I found that you answer is very reasonable and indeed, because the risk or
having this flanks the bacteria avoid those sequences. However, what about
non-palindromic restriction sites? Those are avoided too, but are those as
important as palindromic sequences? The non-symmetric restriction sites will
be target of a single-stranded cleavage or same as palindrome, the enzymes
can cut both DNA strands?

I will really appreciate your input.

Ana Luisa

-- 
Ana Luisa Maldonado-Contreras
PhD Candidate
University of Puerto Rico
Rio Piedras Campus
Microbial Ecology Lab
Phone: 787 764 0000 ext. 4883
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