From stamets1 at aol.com Sun Oct 2 10:47:10 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:34 2005 Subject: identify of microorganisms composing Kambucha Message-ID: <36mklu$bc1@newsbf01.news.aol.com> For those interested in reading about the identity of the organisms composing Kambucha see the Mycologist, Vol 7 (1): 12-13. 1993. paul stamets From maxwell at calshp.cals.wisc.edu Sun Oct 2 21:07:39 1994 From: maxwell at calshp.cals.wisc.edu (maxwell@calshp.cals.wisc.edu) Date: Mon Mar 7 09:15:34 2005 Subject: Growing morel mushrooms References: <9410030140.AA26929@calshp.cals.wisc.edu> Message-ID: <9410030209.AA27447@calshp.cals.wisc.edu> > > > >> I seem to remember reading a paper/abstract somewhere that some morels had > >>a symbiotic (mycorrhizal) relationship with various ferns and grasses. If > >>true then surely this IS a scam of note. > > I think I've seen reports for at least one type of tree, too. I'm pretty sure that Morchella esculenta forms ectomycorrhizae on American Elm, though I can't find where I read this (always misplacing references). Alexopolous says about morels "many if not all are mycorrhial but this has not been proved." > > >I don't think this is the case; if morels do engage in mycorrhizae, then > >its certainly not of the obligate kind. The fact that Morels do so well > >in the wake of disturbance, especially forest fires, seems to rule aginst > >their being obligate mycorrhizal fungi. Certainly, they are not obligate mycorrhizae, since it is possible to isolate vegetative cultures of the fungus simply by plating a piece of the ascocarp on media. > > Under these conditions they operate more like cryophiles- microbes > which thrive best in very cold, nutrient rich soils, but are usually > outcompeted in warmer soils. Whole forests used to be burnt down in Russia > to encourage morels- a practice which surely would not have become popular > if it was necessary to have mycorrhizae. I have to disagree. A stable mycorrhizal reationship should encourage vegetative growth of the fungus. Sex generally occurs when a nutrient source becomes limited. Burning forests would cause the nutrient source of the fungi (the living tree) to be absent. At this point, the organism puts all of its resources into sex, and long distance dispersal. -enjoy. A -- David Maxwell 382 Russell Labs maxwell@calshp.cals.wisc.edu 1630 Linden Drive dlm@plantpath.wisc.edu From mb1gt at sunc.sheffield.ac.uk Mon Oct 3 04:19:51 1994 From: mb1gt at sunc.sheffield.ac.uk (G Turner) Date: Mon Mar 7 09:15:35 2005 Subject: Neurospora expression vectors? Message-ID: <36oibn$rjb@hippo.shef.ac.uk> Does anyone know of the existence of useful expression vectors for overexpression of proteins in N. crassa? Geoff Turner Sheffield G.Turner@sheffield.ac.uk From bjorn at kjemi.tih.no Wed Oct 5 11:18:58 1994 From: bjorn at kjemi.tih.no (Bjorn Ness) Date: Mon Mar 7 09:15:35 2005 Subject: Xylitol - production from xylan (hemicellulose) - fermentation Message-ID: Information needed - please help ! A student group at our college are doing a projectwork on the isue: production of xylitol from xylans (wood-hemicellulose). Can anyone give us information and/or referenses regarding microorganisms producing enzymes for degradation of hemicellulose to xylose and microorganisms producing enzymes witch reduses xylose to xylitol. Please answeare by E-mail. Thank's Bjorn Arne Nass Sør-Trøndelag College bjorn@kjemi.tih.no From margots at wimsey.com Tue Oct 4 20:24:25 1994 From: margots at wimsey.com (Margot Sinclair) Date: Mon Mar 7 09:15:35 2005 Subject: help! need info-odourless compost Message-ID: I really would appreciate it if anyone can provide some information about odourless compost for mushroom farms. In some places, odour is considered an environmental hazard. So I'm trying to find the latest technology available that's environmentally-friendly (ie. odourless). I heard that Pennsylvania and the Netherlands are two sites with new technology. This is not an area in which I specialize . . . so in advance, I thank you for your patience and your assistance. Please respond by e-mail to margots@wimsey.com Thanks! -- margots@wimsey.com From stametsfam at aol.com Wed Oct 5 08:33:03 1994 From: stametsfam at aol.com (Stametsfam) Date: Mon Mar 7 09:15:35 2005 Subject: help! need info-odourless compost References: Message-ID: <36u9uf$ajc@newsbf01.news.aol.com> In article , margots@wimsey.com (Margot Sinclair) writes: Ostrom's Mushroom Farms, here in Olympia, has reduced its odiferousness significantly over the past few years. They collect leachate and re-spray it over the compost continuously during Phase I. This oxygenation of the leachate seems to have a significant impact on ammonifying bacteria. You might want to contact Bill Street Jr. or Sr. at 206-491-1410 to discuss their process. Good luck. Paul Stamets From bncraig at bud.peinet.pe.ca Wed Oct 5 18:38:17 1994 From: bncraig at bud.peinet.pe.ca (Brian N. Craig) Date: Mon Mar 7 09:15:35 2005 Subject: potato newsletter Message-ID: <36v9sq$mso@bud.peinet.pe.ca> A new Newsletter that may interest you. Feel free to subscribe or comment. PRINCE EDWARD ISLAND POTATO NEWSLETTER October 1994 Volume 1, No. 2 This is a newsletter to provide information on extension technology that may be useful to the agriculture sector for potato production. The information will provide ideas and information useful to potato production in the North Eastern parts of North America. As this is a Canadian potato newsletter, the information on products will be those registered for use in Canada. Foreign products will be mentioned to provide the agriculture sector with new technology. Please feel free to read the articles and comment on the content. The Newsletter is designed to help individuals to stay up dated on events and technology. Only through comments will the newsletter be able to provide information that will suit your needs. ---------------------------------------------------------------------------- This issue of the Potato Newsletter will focus on Potato Storage Buildings, Post Harvest Potato Treatment, Potato Seed Field Inspection Standards, Items Wanted To Purchase, Products and Services For Sale. ----------------------------------------------------------------------------- POTATO STORAGE BUILDING: Potato storage buildings should be thoroughly inspected to ensure the potato stores well. The storage building must be able to protect the tubers from freezing while held at low temperatures. The building must be capable of maintaining high levels of humidity to keep the potato from loosing moisture. Because the tuber is "living", the storage must be able to move air and the humidity through the piled tubers. Storage buildings should be inspected each year. What should you check for? Here are a few things to check: 1. Insulation - Insulation can be damaged over the years. If they are not kept under control, rodents can cause major damage to the insulation. Therefore check the storage doors, ceilings and walls to ensure all of the insulation is still intact. Keep in mind that the walls of the storage building should have an insulation factor of R-30 while the ceiling should have an insulation factor of R-40. This level of insulation will keep condensation from collecting on your walls and ceilings. Moisture collecting on the building and falling down onto tubers creates ideal conditions for bacterial soft rot to develop. 2. Vapour Barrier - Check the vapour barrier covering the insulation. With high humidity requirements to store potatoes at, the vapour barrier protects moisture from entering the insulation. If moisture enters the insulation, the insulation ability of the potato storage will be reduced. Make sure the vapour barrier is still in good repair to keep moisture from soaking into the storage insulation. 3. Equipment (Fans & Humidifiers) - High quality storage buildings use fans to circulate air through the stored potato tubers. Stored tubers must have frequent air exchanges to remove heat, carbon dioxide, and excess water vapour. Fans must be in top working order to ensure even temperature and water vapour is evenly distributed throughout the piled potato tubers. Humidifiers may be required to ensure the relative humidity remains between 90-95%. Each year always consider if the fans and humidifiers are matched to meet the greatest demand the storage will be faced with. Will there be more tubers to store this year than last year? If the answer is yes, will the equipment handle the extra harvested tubers this year. Will the fan capacity deliver one (1) cubic foot of air per minute per cwt (hundred weight) of tubers. This should be your minimum fan capacity in your storage building. Make sure all equipment is in good repair. Check bearings, belts and lubricate the equipment before filling the storage. When the potato storage building is filled, make sure that the storage is moving air uniformly throughout the building. A good potato storage building must remove excess condensation from the ceilings and walls. 4. Duct System - The duct system that the fans and humidifiers are connected to should be checked. Check to ensure that none of the ducts are plugged or broken. This can be very crucial to ensure air movement is well distributed through the piled potato tubers. Once the potatoes are placed in storage it becomes extremely difficult to repair or clean a duct system. Keep in mind that the duct system has to be properly sized to the fan and humidifier system to ensure proper air flow through the stored potato tubers. 5. Doors - This is one area that causes many producers problems. Storage building doors must function the same as the walls of the building. They require the same insulation and vapour barriers as the walls have. When the doors are closed, they must seal "tightly". There is more heat lost in storage buildings because of doors not closing tight and providing a good quality seal. Doors are very prone to damage. Trucks, tractors and unloader have caused what would be consider small damage but results in major storage losses such as frozen tubers. Check and keep doors in top repair. 6. Control Panel - This is the "brains" of the storage. If the control panel is not functioning properly, the whole storage could be quickly lost. The control panel is connected to thermocouple which should be checked carefully to ensure they are in good repair and are working properly. Be sure that the thermocouple are placed properly in the storage building and replace andy malfunctioned ones. Before filling the storage make sure the control panel operates the dampers and damper motors to ensure the inside environment will be maintained at the levels required to store the harvested tubers. POST HARVEST POTATO TREATMENT: Potato harvesting usually brings with it the risk of storage diseases. To keep these diseases well under control a potato tuber treatment is recommended. MERTECT (thiabendazole) is the standard treatment to consider for treating tubers going into storage. Mertect controls tuber diseases: Dry Rot (Fusarium spp.), Pocket Rot (Phoma spp.), Silver Scurf (Helminthosporium spp.), Skin Spot (Oospora spp.) and Black Scurf (Rhizoctonia spp.). Mertect will provide a higher quality potato to be marketed as table stock. Through the use of Mertect seed potato producers will be able to preserve potato seed vigour, reduce seed decay and keep the five diseases mentioned above under control. The processing potato will benefit from Mertect. Processing potatoes are stored at a higher temperature than seed and table stock tubers. Warm temperatures are more favourable to disease infection than tubers stored at cooler temperatures. Mertect is sold in Canada as a 45% thiabendazole flowable product. When mixed with water, Mertect disperses easily in the water. The product is applied directly to the tubers as a mist as the potato moves over the bin piler. It is also recommended that tubers for seed and table stock be retreated at time of grading to help protect the tubers during shipment and storage at their destination. Mertect Application : 1. Shake the container of Mertect well. 2. Mix 8 litres of Mertect in 170.21 litres of water. Be sure to add the Mertect to the water. Do NOT add water to Mertect. 3. Keep the solution agitated in the holding tank at all times. This prevents the Mertect from settling out. 4. Apply 2 litres of the suspension per metric tonne of potatoes. Be sure the potatoes are rotating over the conveyor line to ensure total tuber coverage. 5. An ideal nozzle pressure for applying the Mertect mist is 80 to 100 psi. This allows a fine mist that will minimize product run-off on the potato tuber. 6. Always try to mix up a new solution of Mertect each day. Do not let the mixture to stand over night. 7. Always follow the labelled directions on the Mertect container. Mertect Applicator: A minimum of equipment is required to apply a Mertect solution. A small motor and pump is needed that will provide 80 to 100 psi of pressure. A holding tank for the Mertect solution with an agitator system, a pressure regulator (with shut-off valve) and a return bypass hose, a pressure gauge and three hollow cone nozzles (8 to 10 inches from the potatoes) that operate on a swivel to set the spray pattern. The applicator system should be installed at a point on the conveyor where the potatoes are falling or turning over. One good place for the spray system is on the bin piler where the potatoes drop onto the boom conveyor form the dirt eliminator or sizing chain. The potatoes both tumble and fall through the mist at this point. The spray equipment can also be watched carefully by the bin piler operator. Be very careful in locating the Mertect treater where the spray mist will cover canvas-backed belts. Canvas belts tend to shrink or separate if they become wet. Use belts that are completely rubber coated. FIELD STANDARDS FOR CLASSES OF SEED POTATOES PRODUCED ON PRINCE EDWARD ISLAND Pre-Elite: During the growing season, the fields are inspected 3 times by Agriculture Canada Potato Inspectors. With each field inspection, the potato plants must be visably free from any varietal mixture and free from visible symptoms of viruses or other diseases that could affect the quality of the seed. Elite I: During the growing season, the fields are inspected 3 times by federal agriculture inspectors. The potato seed fields must be planted in tuber units - whole seed or cut seed. The first 2 inspection have a tolerance for viruses - there must not be any more than 0.1% visible symptoms of viruses. During the last inspection no visible viruses must be observed. For varietal mixture, there is a zero tolerance during each inspection. Elite II: Durinng the growing season, the fields are inspected 3 times by federal agriculture inspectors. The potato seed must be planted in tuber units - whole seed or cut seed. During the first 2 inspections there must be no more than 0.1% visible symptoms of viruses with no symptoms visible during the last inspection. For varietal mixture, there is a zero tolerance during each inspection. Tolerances for field inspections of Elite III, Elite IV, Foundation and Certified are as follows: Elite III: Inspection 1 .25% Total Viruses; .5% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Inspection 3 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Elite IV: Inspection 1 .5% Total Viruses; 1.0% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .1% Total Viruses; .25% total viruses, wilt, blackleg; 0% Foreign Varieties. Foundation: Inspection 1 .5% Total Viruses; 1.0% total viruses, wilt, blackleg; .1% Foreign Varieties. Inspection 2 .2% Total Viruses; .5% total viruses, wilt, blackleg; .05% Foreign Varieties. Certified: Inspection 1 1.0% any one virus; 2.0% Total Viruses; 3% total viruses, wilt, blackleg; 3% Foreign Varieties. Inspection 2 .5% any one virus; 1% Total Viruses; 2% total viruses, wilt, blackleg; .1% Foreign Varieties. IN UP COMING NEWSLETTERS TUBER STANDARDS FOR SEED SHIPMENTS WILL BE DETAILED. ----------------------------------------------------------------------------- WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! WANTED! Any one wishing to sell Russet Norkotah this year? I have Prince Edward Island buyers who are looking for major sources of this variety. For sales please contact this Newsletter. ----------------------------------------------------------------------------- FOR SALE! FOR SALE! FOR SALE! FOR SALE! I have suppliers of new potato varieties. All seed is Nuclear stock or Pre-Elite. The varieties that are for sale include over 80 potato varieties. These varieties include: ATLANTIC, AC NOVACHIP, AC DOMINO, ALLEGANY, CHIEFTAIN, FONTENOT, GEMCHIP, GOLDRUSH, FRONTIER RUSSET, KATAHDIN, KRANTZ, KENNEBEC, NISKA, NORWIS, REDDALE, RUSSET BURBANK, RUSSET NORKOTAH, SEBAGO, SHEPODY, SNOWDEN, SUPERIOR, YUKON GOLD, D.R. NORLAND, NORCHIP. A hot new variety A7961-1 is also available. For further information on any of these varieties please contact this Newsletter. ----------------------------------------------------------------------------- Genetic Engineering: If you have specific genes in bacteria you wish to introduce into other potato varieties, this newsletter has listings of companies who have the technology and abilities to commercialize this opportunity for you. Confidentiality will be maintained with any genetic requests made by cooperating individuals. ----------------------------------------------------------------------------- If you have products or services that would be of interest to the potato sector, please contact this Newsletter. Comments or suggestions for future Newsletters are welcomed. POTATO NEWS bncraig@bud.peinet.pe.ca From lszabo at PUCCINI.CRL.UMN.EDU Wed Oct 5 16:19:55 1994 From: lszabo at PUCCINI.CRL.UMN.EDU (Les J. Szabo) Date: Mon Mar 7 09:15:35 2005 Subject: USDA National Needs Fellowships Message-ID: <54691.lszabo@puccini.crl.umn.edu> We currently have one, possible two open positions for USDA National Needs Fellowships and we are looking for additional canidates. The tilte of the fellowship program is "Towards new and novel plant resistances to diseases caused by biotrophic fungi: molecular gentices of biotrophic fungal pathogens". Our time frame is extremely short, please contact Dr. Zeyen by Oct. 15, 1994 if you are interested. Ph.D. Fellowships in Plant and Fungal Biotechnology Two, three-year Ph.D. student fellowships from the USDA National Needs Graduate Fellowship Program in Plant Biotechnology are available in the Department of Plant Pathology, University of Minnesota, St Paul. Fellowship stipends are $17,000 annually. Fellowship program research involves plant defense systems active against diseases caused by biotrophic fungi. Research areas are: 1.) development of novel resistances for small grained cereals (oats and barley) using transgenics; 2.) genomic mapping of plant disease resistance genes in legumes using RFLP's; 3.) molecular genetics of biotrophic fungi, including mapping of aviulence genes in rust fungi. Fellowships will be filled within the 1994-95 academic year. Each supports a Ph.D. candidate for 3 years. Fellowship appointments can only be held by United States citizens pursuing a full-time Ph.D. program in plant pathology. Fellowship applicants must meet or exceed all University of Minnesota Graduate School requirements. The University of Minnesota is an equal opportunity educator and employer. For information on the University of Minnesota, the Department of Plant Pathology, and graduate school registration materials contact: Professor Richard J. Zeyen Department of Plant Pathology 495 Borlaug Hall University of Minnesota St. Paul, Minnesota 55108 USA Internet: richz@puccini.crl.umn.edu Phone: 612/625-4754 FAX: 612/625-9728 or Les J. Szabo Research Geneticist/Asst. Professor USDA-ARS Cereal Rust Laboratory Department of Plant Pathology University of Minnesota 1551 Lindig St. St. Paul, MN 55108 Phone: (612)625-3780 FAX: (612)649-5054 lszabo@puccini.crl.umn.edu From bermaa at vccsouth12.its.rpi.edu Wed Oct 5 15:17:51 1994 From: bermaa at vccsouth12.its.rpi.edu (Adam Louis Berman) Date: Mon Mar 7 09:15:35 2005 Subject: Kampucha Message-ID: <36v1lf$bva@usenet.rpi.edu> Is there anyone who can supply information on Kampucha? I am currently researching the reproductional and nutritional capabilities of this organism. If you can offer any help, please reply through email. Thank you From vicka at wrq.com Wed Oct 5 10:01:19 1994 From: vicka at wrq.com (the Littlest Orc) Date: Mon Mar 7 09:15:35 2005 Subject: Puget Sound Wild Mushroom Show Message-ID: <36snmu$am3@news.u.washington.edu> Announcing the 31st Annual Puget Sound Mycological Society W I L D M U S H R O O M S H O W !!! Saturday October 22 (noon to 8 PM) and Sunday October 23 (10 AM to 6 PM) at the Center for Urban Horticulture, University of Washington, 3501 41st Street NE, Seattle, Washington There will be over 500 species of wild mushrooms identified and on display, along with demonstrations about cooking, cultivation, ecology, identification, poisonings, arts and crafts, microscopy, and mushroom lore. Fun for everybody, from mushroom maniacs to anyone who's ever wondered about a toadstool! Cost is $4 for general admission, $2 for students and seniors, and free for children under 12. For more information (or general info about the Puget Sound Mycological Society) send email to vicka@wrq.com. --vicka corey vicka@wrq.com "more fun than a barrel of monkey wrenches!" From mb1jxk at silver.shef.ac.uk Thu Oct 6 07:31:31 1994 From: mb1jxk at silver.shef.ac.uk (J Kennedy) Date: Mon Mar 7 09:15:35 2005 Subject: PCR from fungal colonies Message-ID: <370qn3$f8v@hippo.shef.ac.uk> I am going to be doing some PCR on fungal colonies from plates. As I will be screening a lot of colonies (from a cross of Aspergillus nidulans) I am looking for a quick and reliable method. Any suggestions would be welcome. j.kennedy@sheffield.ac.uk From mycoworld at aol.com Wed Oct 5 22:03:16 1994 From: mycoworld at aol.com (MycoWorld) Date: Mon Mar 7 09:15:35 2005 Subject: Wild Matsutake Harvest Message-ID: <36vpdk$p70@newsbf01.news.aol.com> I am trying to determine how well the wild matsutake (Tricholoma magnivelare, Armillaria ponderosa, A. Caligata) season is doing around the world. Yields have been poor and prices low here in central Oregon. I have a reliable report from Korea that their season is one of the worst on record. I believe that Canada (BC) is doing ok and hear rumors that China and Russia are doing well - can anyone confirm or deny this info? Also, can anyone tell me what is happening in the Japanese retail market in terms of prices and supply compared to previous years? Thanks for any information. Jerry Haugen, The Mushroom Growers' Newsletter MycoWorld@aol.com From harris at bhc.com Thu Oct 6 15:29:24 1994 From: harris at bhc.com (Bob Harris) Date: Mon Mar 7 09:15:35 2005 Subject: Fascism at Work in our Government Message-ID: I just downloaded this and thought all the "mycophiles" might like to investigate this more fully: This may be a bit premature since I have not myself verified exactly what happened, however, I have it on good authority that in mid-to-late-September to DEA sent a written order to ATCC (American Type Culture Center, I believe) demanding that they destroy all their psilocybian mushroom cultures. Evidently the researchers at ATCC carried out the order. It's my understanding that ATCC was one of the only legal sources for such cultures. I'll be trying to get some solid facts on this when I return to on Monday. If anyone has any info please funnel it my way. -richard glen boire The Entheogen Law Reporter P.O. Box 73481 Davis, CA 95617-3481 From Jerome_J_MOTTA at UMAIL.UMD.EDU Fri Oct 7 17:39:16 1994 From: Jerome_J_MOTTA at UMAIL.UMD.EDU (jm102) Date: Mon Mar 7 09:15:35 2005 Subject: ATCC & Psilocybe Message-ID: <9410072239.AA18587@umailsrv1.UMD.EDU> Just to set the record straight regarding Psilocybe and ATCC, the fact is that no one ordered, demanded or otherwise pressured ATCC into de-accessioning Psilocybe from its inventory. Psilocybe is on the list of controlled substances. DEA requires a special "License To Distribute" every controlled substance and imposes a significant financial burden of documentation and justification, including licensing, on both the vendor and the vendee. Even though ATCC is a non-profit organization, it is a business and it has to pay the rent. Simply maintaining living cultures is an expensive proposition in and of itself. There isn't much demand from the scientific community for Psilocybe. When ATCC looked at the distribution figures (sales) of Psilocybe cultures and factored in all the additional costs associated with federal regulation, it decided it was just not cost effective to maintain the culture. De-accessioning of Psilocybe was simply a business decision and not the result of coercion by anyone or any agency, government or otherwise. If more of you folks into schroons had bought cultures it would still be available. From brianmc at aol.com Fri Oct 7 16:02:04 1994 From: brianmc at aol.com (BrianMc) Date: Mon Mar 7 09:15:35 2005 Subject: Fascism at Work in our Government Message-ID: <374d0c$3up@newsbf01.news.aol.com> >I just downloaded this and thought all the "mycophiles" >might like toinvestigate this more fully: > >This may be a bit premature since I have not myself >verified exactly what happened, however, I have it on >good authority that in mid-to-late-September to DEA sent >a written order to ATCC (American Type Culture Center, I >believe) demanding that they destroy all their psilocybian >mushroom cultures. Evidently the researchers at ATCC >carried out the order. It's my understanding that ATCC was >one of the only legal sources for such cultures. > >I'll be trying to get some solid facts on this when I return >to on Monday. If anyone has any info please funnel it my way. Behavior such as this should not be tolerated by the scientific community. There are several species of rare Psilocybe of which only one or two specimens have ever been recorded, the ONLY examples of which now reside in culture collections overseas, and which are now in a very sad state due to the repressive paranoia surrounding the genus, a reputation which it hardly deserves. Are we to not include specimens simply because our govenrment has an excessive fear of the possible psycotrophic content? ---------------------------------------------------------- | Brian McNett e-mail: | Editor: MycoInfo Submissions: | Promoting Mycology in the Online Community From lmelvill at uoguelph.ca Fri Oct 7 10:22:52 1994 From: lmelvill at uoguelph.ca (Lewis Melville) Date: Mon Mar 7 09:15:35 2005 Subject: Fascism at Work in our Government References: Message-ID: <373p4c$gtg@nermal.cs.uoguelph.ca> Bob Harris (harris@bhc.com) wrote: : I just downloaded this and thought all the "mycophiles" might like to : investigate this more fully: : This may be a bit premature since I have not myself verified exactly : what happened, however, I have it on good authority that in : mid-to-late-September to DEA sent a written order to ATCC (American : Type : Culture Center, I believe) demanding that they destroy all their : psilocybian mushroom cultures. Evidently the researchers at ATCC : carried out the order. It's my understanding that ATCC was one of the : only legal sources for such cultures. This seems like a futile task. It's highly unlikely that anyone interested in culturing psilocybin for questionable purposes would need to obtain their material from the ATCC. This would be like destroying hemp seed kept in a seed bank as a means to eliminating street use of cannibis. Question: Should scientists carry out this type of order or resort to civil disobedience? : I'll be trying to get some solid facts on this when I return to on : Monday. If anyone has any info please funnel it my way. : -richard glen boire : The Entheogen Law Reporter : P.O. Box 73481 : Davis, CA 95617-3481 From rarnold at teleport.com Thu Oct 6 19:50:16 1994 From: rarnold at teleport.com (Ralph D. Arnold) Date: Mon Mar 7 09:15:35 2005 Subject: Here is An Update Message-ID: <78407.rarnold@teleport.com> Several days ago, I posted a news article asking for responses, in order to obtain an EMAIL-based contact list for myco-fans. Below is the latest information I have received. Please update me with additions or changes. The only request is that the organization must be reachable by internet email. Other list can include snail-mail-only groups. My address is at the end of this posting. ////////////////////////////////////////////////////////////////////////// INTERNATIONAL ORGANIZATIONs PUGGEHATTEN-MYCOLOGICAL SOCIETY (Puggehatten is one of the local words for fungi in general. Best translated to "Toads Hat" or something like it.) contact person: Sigvard Svensson email address: Sigvard.Svensson@botmus.lu.se fax number: +46 46 10 42 34 phone number: +46 46 10 40 61 mailing address: Botanical Museum Oestra Vallgatan 18 S-223 61 Lund Sweden description: Local society working with the fungi of SCANIA (Southernmost counties in SWEDEN). We are especially interested in surveying the redlisted species and threatened nemoral biotopes. We are NOT devoted to mycophagy, although we pick the edibles we pass on forays. At the moment we are ca 200 members and issues a "members journal" in swedish, called PUGGEHATTEN with about 100 pages a year divided into four issues. Current annual fee is SEK 75:- *************************************************************************** BRITISH MYCOLOGICAL SOCIETY Contact Person: Dr. S.T. Moss (General Secretary) Email Address: moss@uk.ac.portsmouth.csovax Fax Number: 0705 842070 Phone Number: 0705 842024 Mailing Address: School of Biological Science King Henry Building University of Portsmouth King Henry 1 Street Portsmouth Hants, PO1 2DY, UK Description: Membership is provided for at Associate (Amateur) and Professional levels. A quarterly journal of general mycology - the Mycologist - can be subscribed to without membership of the Society. This journal includes recipes and articles of general interest. Both Associates and Professionals receive the Mycologist as part of their membership package. ////////////////////////////////////////////////////////////////////////// AMERICAN ORGANIZATIONS Fungus Federation of Santa Cruz Contact Person: Nathan Wilson Email Address: nathan@cse.ucsc.edu Phone Number: 408 684-2275 Mailing Address: 1305 East Cliff Dr Santa Cruz, CA 95062 Description: The amateur mushroom group in the Santa Cruz area affiliated with the North American Mycological Association. We meet on the third Wednesday of each month at the Santa Cruz City Museum of Natural History at above mailing address. **************************************************************************** Ohio Mushroom Society Contact Person: Jerry Pepera at gerald.pepera@ab.com Dick Doyle at doyle@cc.denison.edu Fax Number: 216 646-4343 Phone Number: 216 354-4774 (home) 216 646-4277 (work) Mailing Address: 10489 Barchester Dr Concord, OH 44077 Description: Organizes 3-4 mushroom forays per year all over Ohio. Public is always welcome. Evenets feature mushroom taste sampling, workshops, slide show, giveaways, and an organized lunch. Memebrship is $10.00 per year, which includes 6 newsletters, taxonomy service, program materials, and article database (under construiction) ***************************************************************************** MYCOINFO Newsletter Contact Person: brianmc@aol.com Description: This is an interesting newsletter produced free-of-charge via email. To subscribe, send a short message to above email address. ///////////////////////////////////////////////////////////////////////////////// COMMERCIAL ORGANIZATIONS The Mushroom Growers' Newsletter Contact People: Jerry & Trish Haugen Email Address: mycoworld@aol.com Fax Number: 503 883-3273 Phone Number: 503 883-3719 Mailing Address: P.O. Box 5065 Klamath Falls, OR 97601 Description: This monthly (on paper) targets primarily specialty mushroom growers (mostly small-scale growers, hobby growers, or those interested in it). Subjects include: cultivation of various species, marketing info, market prices (San Francisco and New York), environmental control, profiles of commercial growers, production statistics, equipment, book reviews, and sources of information and supplies. ***************************************************************************** Abbey Lane Laboratories Contact Person: Steve Carpenter Email Address: microbe@csos.orst.edu Mailing Address: P.O. Box 1665 Philmath, Oregon Description: Identification of molds for industry and agriculture. Teaching of classes on mushroom ident. in Corvallis ***************************************************************************** Florida Mycology Research Center (FMRC) Contact Person: Stephen L. Peele (curator) Email Address: 72253.1553@compuserve.com Phone Number: 904 327-4378 Mailing Address: P.O. Box 8104 Pensacola, FL 32505 Description: FMRC carries the world's largest mushroom spore and live culture collection. Publishes "The Mushrrom Culture, the Journal of Mushroom Cultivation (TMC)". Sells videos and publications on mushrooms not found elsewhere. Handles four catalogs ($10US each): "MAIN" - growing supplies, spores, and cultures of edible fungi. "RED" - spores and cultures of all types of mushrooms. "CHROMATOGRAPH" - supplies for doing extrations. "PLANT TISSUE CULTURE" - everything for tissue culturing ***************************************************************************** Fungi Perfecti Contact Person: Paul Stamets Email Address: stamets1@aol.com Fax Number: 206 426-9292 Phone Number: 206 426-9377 Mailing Address: P.O. Box 7634 Olympia, WA 98507 Description: FP offers an extremely-wide choice of supplies, books, seminars, accessories, and species/straining for the hobbiest and commercial grower. Contact them for details. ********************************************************************************** Thank you, P.S. I am in no way affiliated with ANY of these or other groups - I'm simply interested in mycology and enjoy the fast contact mode of email. Ralph D. Arnold P.O. Box 889 Oregon City, OR 97045 rarnold@teleport.com From sannis at uoguelph.ca Fri Oct 7 20:53:43 1994 From: sannis at uoguelph.ca (Seanna Annis) Date: Mon Mar 7 09:15:35 2005 Subject: Leptosphaeria maculans on Arabidopsis References: <36bpks$787@news.ycc.yale.edu> Message-ID: <374u37$5i3@nermal.cs.uoguelph.ca> Larry Ilag (llilag@minerva.cis.yale.edu) wrote: : Does anyone know of research articles documenting Leptosphaeria maculans : (Phoma lingam; causal agent of blackleg) infection on Arabidopsis thaliana? : Thanks in advance. : Larry Ilag I am working on this fungus for my PhD. I have never heard of it naturally or artifically attacking Arabidopsis. I suggest getting a few highly virulent cultures and try it. It may work, I don't know off the top of my head how close Arabidopsis is to Crucifers. Dr. Paul Williams, at Michigan State University has a large collection of isolates, and if this host-pathogen interaction has ever been tried he might know. Good luck Seanna sannis@uoguelph.ca From harris at bhc.com Fri Oct 7 20:28:03 1994 From: harris at bhc.com (Bob Harris) Date: Mon Mar 7 09:15:35 2005 Subject: ATCC & Psilocybe References: <9410072239.AA18587@umailsrv1.UMD.EDU> Message-ID: In article <9410072239.AA18587@umailsrv1.UMD.EDU> Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) writes: > > Just to set the record straight regarding Psilocybe and ATCC, the fact is > that no one ordered, demanded or otherwise pressured ATCC into de-accessioning > Psilocybe from its inventory. Psilocybe is on the list of controlled > substances. Just to set the record straight: Psilocybe is NOT a controlled substance. Psilocybin and psilocin ARE. The musrhoom has never been controlled under federal law, and only a few states, California being one of them, has ever specified a species of mushroom being illegal. ATCC has always had the requirements that you fill out special forms for these species, but you were still able to order them. As to the business aspect, there are thousands of cultures that ATCC maintains for the genetic diversity reasons alone. Many of those have never sold well. From sannis at uoguelph.ca Fri Oct 7 20:59:57 1994 From: sannis at uoguelph.ca (Seanna Annis) Date: Mon Mar 7 09:15:35 2005 Subject: PCR from fungal colonies References: <370qn3$f8v@hippo.shef.ac.uk> Message-ID: <374uet$5i3@nermal.cs.uoguelph.ca> J Kennedy (mb1jxk@silver.shef.ac.uk) wrote: : I am going to be doing some PCR on fungal colonies from : plates. As I will be screening a lot of colonies : (from a cross of Aspergillus nidulans) I am looking for : a quick and reliable method. Any suggestions would be : welcome. : j.kennedy@sheffield.ac.uk : In our lab we have been using a boiling prep method, very little time involved. I believe one of the methods can be found in Biotechniques. I have not used the method myself, but many people in our lab have, and it usually worked well. Good luck. Seanna sannis@uoguelph.ca From rwinder at PFC.Forestry.CA Fri Oct 7 12:05:02 1994 From: rwinder at PFC.Forestry.CA (Richard Winder) Date: Mon Mar 7 09:15:35 2005 Subject: Wild Matsutake Harvest References: <36vpdk$p70@newsbf01.news.aol.com> Message-ID: <1994Oct7.170502.23027@spruce.pfc.forestry.ca> In article <36vpdk$p70@newsbf01.news.aol.com>, mycoworld@aol.com (MycoWorld) writes: >I am trying to determine how well the wild matsutake (Tricholoma >magnivelare, Armillaria ponderosa, A. Caligata) season is doing around the >world. Yields have been poor and prices low here in central Oregon. I >have a reliable report from Korea that their season is one of the worst on >record. I believe that Canada (BC) is doing ok and hear rumors that China >and Russia are doing well - can anyone confirm or deny this info? I've heard that there really aren't that many in BC this year- a few spots maybe OK due to our diverse geography, but overall the harvest seems to be low according to at least one harvester I talked too. That could just be here on Vancouver Island, though. Prices also seem to be coming down, according to this gentleman, though I can't confirm that- there was somewhat of a gold-rush mentality that is perhaps simmering down. Maybe all the good hunters have staked out their claims and purchased very good flashlights :-) -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From geomay at MOLBIO.CBS.UMN.EDU Sun Oct 9 15:18:32 1994 From: geomay at MOLBIO.CBS.UMN.EDU (Georgiana May) Date: Mon Mar 7 09:15:36 2005 Subject: pcr from colonies Message-ID: <9410092016.AA23243@molbio.cbs.umn.edu> We use Lee's method (BioTechniques 15, Sept.1993). We start with 3 small squares of mycelium, each about 1-1.5 mm sq. and trim away agar underneath. These go into 0-ring Eppendorf tubes with 50 uL extraction buffer. We follow the method and then use 2 uL/25 uL reaction. It comes out about right for Coprinus cinereus, sometimes a 1:10 dilution works better. The mycelium is freshly grown and the squares are removed about 1 cm back from the colony edge. Georgiana May From TOM at BIO.TAMU.EDU Mon Oct 10 13:31:14 1994 From: TOM at BIO.TAMU.EDU (Tom Adams) Date: Mon Mar 7 09:15:36 2005 Subject: Mycology FAQ (long) Message-ID: ------------------------------------------------------------------------ The BIONET.Mycology FAQ V 1.0 October 10, 1994 ------------------------------------------------------------------------ This is version 1.0 of the BIONET.MICROBIOLOGY FAQ (frequently asked questions). This FAQ is adapted from one supplied by the MICROBIOLOGY newsgroup and also addresses questions and comments raised by the Mycology newsgroup users. Additional suggestions for future postings should be sent to: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu The FAQ will be posted once a month to BIONET.MYCOLOGY, as close to the beginning of the month as possible. The FAQ is organized as follows: I. What is bionet.microbiology II. How to access bionet.microbiology III. How to retrieve old articles IV. How and what to post in bionet.microbiology V. Related newsgroups and bulletin boards VI. Other internet sources of interest to microbiologists VII. Actual frequently asked questions VIII. Miscellaneous I. WHAT IS BIONET.MYCOLOGY ------------------------------- bionet.mycology is an internet newsgroup aimed at the discussion and information exchange of any relevant mycologyical topic. The newsgroupis in the bionet sector and is accessible to subscription via electronicmail and/or directly from newsservers carrying the bionet newsgroups. The newsgroup, bionet.mycology, is intended as a forum for scientific discussions and questions regarding all aspects of the mycological sciences. Topics to be discussed include such diverse aspects of fungal biologyt as genetics, molecular biology, evolution, biochemistry, host-parasite relationships, pathology, physiology, biotechnology, pathogenesis, taxonomy, and the teaching of mycology. The newsgroup also provides a forum for announcing meetings, funding sources, job opportunities, and pointers to other information sources such as the fungal genetics stock center world wide web page. In addition, the newsgroup encourages posting of abstracts for papers that have been accepted for publication in peer reviewed journals. II. HOW TO ACCESS BIONET.MYCOLOGY -------------------------------------- The newsgroup is in the bionet sector and is accessible from your local newsserver (NNTP server), assuming it carries the bionet newsgroups. You also can subscribe to bionet.microbiology via electronic mail. 1) Access by NNTP server The newsgroup is accessible as bionet.mycology by NNTP servers carrying bionet newsgroups. If your NNTP server does not have bionet.mycology, it is likely that your local news administrator has overlooked this newsgroup. Please contact your local news administrator concerning the addition of this newsgroup to your local newsserver (see below). 2) Access by e-mail Everyone in the AMERICAS and the PACIFIC RIM who has no access to a newsserver and wishes to subscribe to bionet.mycology can send an e-mail to: biosci-server@net.bio.net with the following text in the body of the message: subscribe mycology If you are located in EUROPE, AFRICA, OR CENTRAL ASIA and wish to subscribe to bionet.microbiology, send an e-mail to: MXT@dl.ac.uk with the following text in the body of the message: SUB bionet-news.bionet.mycology 3) General BIOSCI/bionet information If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: biosci-server@net.bio.net with info usinfo in the body of your message. If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in Europe, Africa or Asia, send an e-mail from your account to: biosci-server@net.bio.net with info ukinfo in the body of your message. You will receive a file with instructions of how to access the bionet.* newsgroups and furthermore find pointers to other useful information. Dave Kristofferson, the BIOSCI/bionet Manager, and others have done an excellent job in compiling a list of helpful information which are >=a must<= to everybody not familiar with the internet and netnews. This BIOSCI/bionet FAQ will cover all aspects concerning access to newsgroups, subscription, cancellation of subscription, how to post articles, what to post and not to post, how to reply to posts. I strongly recommend everyone not yet familiar with netnews and e-mail subscription to take a few minutes to familiarize themselves with the BIOSCI/bionet FAQ document. The FAQ can be retrieved by sending an e-mail to: biosci-server@net.bio.net with info faq in the body of your message. III. HOW TO RETRIEVE OLD ARTICLES ---------------------------------- 1) Access of bionet.mycology archives by anonymous FTP and gopher Archives for bionet.microbiology can be accessed by anonymous FTP at net.bio.net [134.172.2.69] in the pub/BIOSCI/MYCOLOGY directory. Note that files are ordered by date, and that the filenames are case sensitive. The same archived files are accessible via Gopher using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the MYCOLOGY directory. Please see the BIOSCI/bionet faq for details (see above). IV. HOW AND WHAT TO POST IN BIONET.MYCOLOGY ------------------------------------------------ Any message or post concerning mycology, or any aspect of interest to the mycology community are welcome in this newsgroup, provided they are scientific in nature or are of scientific interest. Some technical guidelines to posting messages to the bionet newsgroups are described in the BIOSCI/bionet faq (see above), and anybody not certain of how and what to post should become familiar with the document. If you like to post to the newsgroup with your favorite newsreader, please follow the instructions of your newsreader of how to post an article. If you like to post to the newsgroup by e-mail, and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: mycology@net.bio.net If you like to post to the newsgroup by e-mail, and are located in Europe, Africa or Asia, send an e-mail from your account to: mycology@daresbury.ac.uk V. RELATED NEWSGROUPS AND BULLETIN BOARDS ------------------------------------------ 1) Newsgroups A number of other newsgroups exist in the bionet.* sector which may be of general interest to mycologists. These newsgroups are: bionet.prof-society.ascb bionet.prof-society.faseb bionet.immunology bionet.molbio.evolution bionet.molbio.methds-reagnts bionet.molbio.yeast bionet.mcrobiology bionet.protista bionet.software.www Anybody interested in accessing these newsgroup can do so as described above. VI. OTHER INTERNET SOURCES OF INTEREST ---------------------------------------------------------- Under construction; a few select entries are: 1) e-mail -------- dFLASH server The dFLASH Group has a new electronic mail server that allows GENBANK and PIR similarity searches with the FLASH algorithm. Further information can be obtained by sending: send help in the body of the message to dflash@watson.ibm.com Make sure you have dflash as subject header. 2) Anonymous FTP ---------------- E. coli Database Manfred Kroeger's E. coli datasets are available from the EMBL FTP site ftp.embl-heidelberg.de NIH Forms The ftp site is: ftp.u.washington.edu The directory is: /pub/user-supported/templates 3) Gopher/WAIS/WWW -------------------- ATCC The name of the host computer at the AMERICAN TYPE CULTURE COLLECTION (ATCC) used to access catalogue information has been changed from atcc.org to culture.atcc.org. This change of name affects using gopher to access information describing research materials available from the ATCC. You must point your gopher client to culture.atcc.org (gopher culture.atcc.org). The change also affects using telnet to connect to the ATCC Recombinant Materials Database (clones, vectors, recombinant libraries, transformation hosts, and oligonucleotides) and searching using IRX (telnet culture.atcc.org, username , password ). Candida WWW and gopher severs with information about Candida albicans molecular biology. The servers share most of their text data. The WWW server has additional images and diagrams. To access them: Point your gopher client to: alces.med.umn.edu There is a directory with the Candida information. The URL for the WWW server is: http://alces.med.umn.edu/start.html There are links to the Candida information and other information on the server on that page. Case Western Department of Biochemistry, Case Western Reserve University School of Medicine WWW server with the following URL http://biochemistry.bioc.cwru.edu:80/ E. coli The E. coli genetic stock center has a Web server: http://cgsc.biology.yale.edu/cgsc.html Barbara Bachman's E. coli culture collection can be accessed by gopher under: cgsc.biology.yale.edu This resource can be used more fully if you can hack the SYBASE program, you can telnet directly and do more powerful searches. The CGSC Gopher files are generated periodically from the public portions of the E. coli Genetic Stock Center (CGSC) database. They include information about strains, mutations, genes, and references. We assume that these files will be used primarily for searching for strains with specific mutations or genotype combinations and secondarily for examining stock center information on mutations and genes. The linkage map is being revised, and the list of genes and coordinates in the "Map" file represent the coordinates currently in the database. Access to the "external version" of the CGSC relational database itself is provided either as a menu option (#5) on this gopher (See sub menu item "How to Access..." and "CGSC Database") or by direct telnet to cgsc.biology.yale.edu To gain direct access, you must obtain a password and login as guest. Send e-mail to mary@cgsc.biology.yale.edu to obtain the current password for guest. --Using the CGSC Gopher v. Querying the CGSC Database-- The subset of information contained in the CGSC-gopher file can be accessed by text searches via IUWais. (See IUWAIS search features on the "2. CGSC Files and Database" menu.) You may also directly query the CGSC relational database. If your query is not easily formulated as a simple Boolean search or if a Wais search returns far too many instances to examine one by one, going to the form-based interface of the database will allow you to specify the query in a way that will limit the returns to those of most interest to you. The About file of the submenu provides examples of both types of queries. Read the DBAccess file on the submenu to learn how to navigate between data fields and menu bar and between object-forms. For more detailed instructions, contact Mary at address below for users' guide. Strains bearing CGSC numbers (the only strains present in the flat file) are available from the stock center. The stock center and the database development are supported by the National Science Foundation. For more information about the database or to request strains, contact Mary Berlyn at CGSC: mary@cgsc.biology.yale.edu Fungal Genetics This information source can be found under the following URL: http://kufacts.cc.ukans.edu/cwis/units/fgsc/main.html WWW server at Keck-IBT filamentous fungal databases URL: http://keck.tamu.edu/ibt.html or gopher://keck.tamu.edu Saccharomyces genomic information resource URL: http://genome-www.stanford.edu/ GenBank A searchable GenBank database can be accessed by addressing your gopher client to ftp.bio.indiana.edu, and looking under Genbank-Sequences/ GenomeNet GenomeNet is a Japanese computer network for genome research and related research areas in molecular and cellular biology. It can be accessed by WWW as: http://www.genome.ad.jp Microbiology The WWW Virtual Library >=Microbiology (Biosciences)<= is accessible at: http://golgi.harvard.edu/biopages/micro.html Protist Images Protist Image Data provides pictures and short descriptions of selected protist genera, especially those genera whose species are frequently used as experimental organisms or are important in studies of organismal evolution. The prerelease version of this database can be accessed through the WWW at the following URL: http://megasun.bch.umontreal.ca/protists/protists.html VII. MISCELLANEOUS -------------------- ACKNOWLEDGMENTS ---------------- This FAQ is adapted from a FAQ generated by Martin latterich, University of California, Berkeley. Thanks to Dave Kristofferson and the >=bionetters<= for compiling an excellent and comprehensive BIOSCI/bionet faq, which served as the basis to the technical subscription information in this MYCOLOGY FAQ in sections II. and III. Also many thanks to Keith Robinson, Harvard University, for much information on internet resources and Jim Graham, Indiana, for contributing Barbara Bachman's E. coli culture collection information. ------------------------------------------------------------------------ More information is always welcome. Please mail your suggestions,criticism and information to one of the following Discussion Leaders for bionet.mycology: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu Tom Adams Texas A&M University Leland Ellis Texas A&M University Greg May Baylor College of Medicine Tom Adams Department of Biology Texas A&M University College Station, TX 77843 409-845-1468 Tom@bio.tamu.edu From thsiang at uoguelph.ca Tue Oct 11 07:51:39 1994 From: thsiang at uoguelph.ca (Tom Hsiang) Date: Mon Mar 7 09:15:36 2005 Subject: AVAILABLE: POST-DOC IN PLANT PATHOLOGY Message-ID: <37e1or$opv@nermal.cs.uoguelph.ca> AVAILABLE: POST-DOC IN PLANT PATHOLOGY (October 1994) PLANT PATHOLOGY Post-Doctoral Research Associate. A position is available in the Department of Environmental Biology at the University of Guelph in Ontario. The major project examines the use of a fungal antagonist to control snow mould disease of turfgrass. There are a couple of smaller projects also involving turfgrass fungal pathogens. A good background in field-oriented plant pathology is required. The appointment is available immediately for a 12-month period with possibilities of extension. The salary is $25,000 per year with an additional 13% in benefits which covers prescription drugs, vision care and some supplementary health care benefits. Interested applicants should send a resume (with list of references) and unofficial copies of University transcripts to: Dr. T. Hsiang, Dept. Environ. Biol., Univ. Guelph, Guelph, Ontario, N1G 2W1, Canada. Phone 519-824-4120 ext. 2753. FAX 519-837-0442. E-mail THSIANG@EVBHORT.UOGUELPH.CA. From an524 at FreeNet.Carleton.CA Wed Oct 12 10:44:11 1994 From: an524 at FreeNet.Carleton.CA (Jeffrey Ehlinger) Date: Mon Mar 7 09:15:36 2005 Subject: Help with culture please (newbie) Message-ID: My professor got some spores for Christmas this past year and he asked me to start them for him. The instructions were in Russian, so we first had to have them translated. The species is "Boletus Edgilus" (the spelling could be wrong). After two weeks, there is many white moldy looking patches on\ the leaf mediumm. I have two trays that are being used. I have lowered the moisture content on one of them to try to get rid of the mold looking stuff. I wonder is this the form the mycelium takes ? Is that what I'm seeing.? In two weeks, I will cut large holes in the plastic bag keeping the moisture in, then hopefully I will get some results. Please e-mail me if you have any idea on how to culture Boletus edgilus. -Jeff PS- the medium should have been completely sterilized when I autocl;aved it at 120C for twenty minutes. From lionel at techlink.fr Tue Oct 11 18:52:04 1994 From: lionel at techlink.fr (Lionel Benhamou) Date: Mon Mar 7 09:15:36 2005 Subject: Myconet Message-ID: <36863.142953160@techlink.techlink.fr> I tried two times to join Myconet without success. Am I the only one ? A bientot. lionel@techlink.fr ========================================================================== TechnoLink - FC Server - France | Renseignements : info@techlink.fr (33-1) 49 83 86 49 | Fax :(33-1) 49 83 81 17 ========================================================================== From hong at mbfys.kun.nl Wed Oct 12 14:51:18 1994 From: hong at mbfys.kun.nl (Hong Zhou) Date: Mon Mar 7 09:15:36 2005 Subject: Recycled compost References: <35unmj$pvo@terrazzo.lm.com> Message-ID: The logic of fining the company is that they should not pollute the environment by adding even more nitrogen rich substrates to the soil. In Holland we have a law which forbids mushroom farmers to put the wasted substrate on the soil, because the sils are allready heavily polluted with nitrogen from the acid rain. Especially on poor soils there is a richdom of natural plants (and mushrooms!) From etj at anthro.umass.edu Thu Oct 13 18:11:17 1994 From: etj at anthro.umass.edu (Eric Jones) Date: Mon Mar 7 09:15:36 2005 Subject: subscription info Message-ID: <01HI8LMY8KAQAPUF9G@phobos.ucs.umass.edu> Please send me any information you have on this conference From TOM at BIO.TAMU.EDU Thu Oct 13 14:44:00 1994 From: TOM at BIO.TAMU.EDU (Tom Adams) Date: Mon Mar 7 09:15:36 2005 Subject: newsgroup info Message-ID: ------------------------------------------------------------------------ The BIONET.Mycology FAQ V 1.0 October 10, 1994 ------------------------------------------------------------------------ This is version 1.0 of the BIONET.MICROBIOLOGY FAQ (frequently asked questions). This FAQ is adapted from one supplied by the MICROBIOLOGY newsgroup and also addresses questions and comments raised by the Mycology newsgroup users. Additional suggestions for future postings should be sent to: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu The FAQ will be posted once a month to BIONET.MYCOLOGY, as close to the beginning of the month as possible. The FAQ is organized as follows: I. What is bionet.microbiology II. How to access bionet.microbiology III. How to retrieve old articles IV. How and what to post in bionet.microbiology V. Related newsgroups and bulletin boards VI. Other internet sources of interest to microbiologists VII. Actual frequently asked questions VIII. Miscellaneous I. WHAT IS BIONET.MYCOLOGY ------------------------------- bionet.mycology is an internet newsgroup aimed at the discussion and information exchange of any relevant mycologyical topic. The newsgroupis in the bionet sector and is accessible to subscription via electronicmail and/or directly from newsservers carrying the bionet newsgroups. The newsgroup, bionet.mycology, is intended as a forum for scientific discussions and questions regarding all aspects of the mycological sciences. Topics to be discussed include such diverse aspects of fungal biologyt as genetics, molecular biology, evolution, biochemistry, host-parasite relationships, pathology, physiology, biotechnology, pathogenesis, taxonomy, and the teaching of mycology. The newsgroup also provides a forum for announcing meetings, funding sources, job opportunities, and pointers to other information sources such as the fungal genetics stock center world wide web page. In addition, the newsgroup encourages posting of abstracts for papers that have been accepted for publication in peer reviewed journals. II. HOW TO ACCESS BIONET.MYCOLOGY -------------------------------------- The newsgroup is in the bionet sector and is accessible from your local newsserver (NNTP server), assuming it carries the bionet newsgroups. You also can subscribe to bionet.microbiology via electronic mail. 1) Access by NNTP server The newsgroup is accessible as bionet.mycology by NNTP servers carrying bionet newsgroups. If your NNTP server does not have bionet.mycology, it is likely that your local news administrator has overlooked this newsgroup. Please contact your local news administrator concerning the addition of this newsgroup to your local newsserver (see below). 2) Access by e-mail Everyone in the AMERICAS and the PACIFIC RIM who has no access to a newsserver and wishes to subscribe to bionet.mycology can send an e-mail to: biosci-server@net.bio.net with the following text in the body of the message: subscribe mycology If you are located in EUROPE, AFRICA, OR CENTRAL ASIA and wish to subscribe to bionet.microbiology, send an e-mail to: MXT@dl.ac.uk with the following text in the body of the message: SUB bionet-news.bionet.mycology 3) General BIOSCI/bionet information If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: biosci-server@net.bio.net with info usinfo in the body of your message. If you like to know more about e-mail subscription and other bionet.* newsgroups and are located in Europe, Africa or Asia, send an e-mail from your account to: biosci-server@net.bio.net with info ukinfo in the body of your message. You will receive a file with instructions of how to access the bionet.* newsgroups and furthermore find pointers to other useful information. Dave Kristofferson, the BIOSCI/bionet Manager, and others have done an excellent job in compiling a list of helpful information which are >=a must<= to everybody not familiar with the internet and netnews. This BIOSCI/bionet FAQ will cover all aspects concerning access to newsgroups, subscription, cancellation of subscription, how to post articles, what to post and not to post, how to reply to posts. I strongly recommend everyone not yet familiar with netnews and e-mail subscription to take a few minutes to familiarize themselves with the BIOSCI/bionet FAQ document. The FAQ can be retrieved by sending an e-mail to: biosci-server@net.bio.net with info faq in the body of your message. III. HOW TO RETRIEVE OLD ARTICLES ---------------------------------- 1) Access of bionet.mycology archives by anonymous FTP and gopher Archives for bionet.microbiology can be accessed by anonymous FTP at net.bio.net [134.172.2.69] in the pub/BIOSCI/MYCOLOGY directory. Note that files are ordered by date, and that the filenames are case sensitive. The same archived files are accessible via Gopher using net.bio.net as your gopher server. Gopher also allows you to view the individual messages within each monthly archive file. The files are in the MYCOLOGY directory. Please see the BIOSCI/bionet faq for details (see above). IV. HOW AND WHAT TO POST IN BIONET.MYCOLOGY ------------------------------------------------ Any message or post concerning mycology, or any aspect of interest to the mycology community are welcome in this newsgroup, provided they are scientific in nature or are of scientific interest. Some technical guidelines to posting messages to the bionet newsgroups are described in the BIOSCI/bionet faq (see above), and anybody not certain of how and what to post should become familiar with the document. If you like to post to the newsgroup with your favorite newsreader, please follow the instructions of your newsreader of how to post an article. If you like to post to the newsgroup by e-mail, and are located in the AMERICAS or the PACIFIC RIM, send an e-mail from your account to: mycology@net.bio.net If you like to post to the newsgroup by e-mail, and are located in Europe, Africa or Asia, send an e-mail from your account to: mycology@daresbury.ac.uk V. RELATED NEWSGROUPS AND BULLETIN BOARDS ------------------------------------------ 1) Newsgroups A number of other newsgroups exist in the bionet.* sector which may be of general interest to mycologists. These newsgroups are: bionet.prof-society.ascb bionet.prof-society.faseb bionet.immunology bionet.molbio.evolution bionet.molbio.methds-reagnts bionet.molbio.yeast bionet.mcrobiology bionet.protista bionet.software.www Anybody interested in accessing these newsgroup can do so as described above. VI. OTHER INTERNET SOURCES OF INTEREST ---------------------------------------------------------- Under construction; a few select entries are: 1) e-mail -------- dFLASH server The dFLASH Group has a new electronic mail server that allows GENBANK and PIR similarity searches with the FLASH algorithm. Further information can be obtained by sending: send help in the body of the message to dflash@watson.ibm.com Make sure you have dflash as subject header. 2) Anonymous FTP ---------------- E. coli Database Manfred Kroeger's E. coli datasets are available from the EMBL FTP site ftp.embl-heidelberg.de NIH Forms The ftp site is: ftp.u.washington.edu The directory is: /pub/user-supported/templates 3) Gopher/WAIS/WWW -------------------- ATCC The name of the host computer at the AMERICAN TYPE CULTURE COLLECTION (ATCC) used to access catalogue information has been changed from atcc.org to culture.atcc.org. This change of name affects using gopher to access information describing research materials available from the ATCC. You must point your gopher client to culture.atcc.org (gopher culture.atcc.org). The change also affects using telnet to connect to the ATCC Recombinant Materials Database (clones, vectors, recombinant libraries, transformation hosts, and oligonucleotides) and searching using IRX (telnet culture.atcc.org, username , password ). Candida WWW and gopher severs with information about Candida albicans molecular biology. The servers share most of their text data. The WWW server has additional images and diagrams. To access them: Point your gopher client to: alces.med.umn.edu There is a directory with the Candida information. The URL for the WWW server is: http://alces.med.umn.edu/start.html There are links to the Candida information and other information on the server on that page. Case Western Department of Biochemistry, Case Western Reserve University School of Medicine WWW server with the following URL http://biochemistry.bioc.cwru.edu:80/ E. coli The E. coli genetic stock center has a Web server: http://cgsc.biology.yale.edu/cgsc.html Barbara Bachman's E. coli culture collection can be accessed by gopher under: cgsc.biology.yale.edu This resource can be used more fully if you can hack the SYBASE program, you can telnet directly and do more powerful searches. The CGSC Gopher files are generated periodically from the public portions of the E. coli Genetic Stock Center (CGSC) database. They include information about strains, mutations, genes, and references. We assume that these files will be used primarily for searching for strains with specific mutations or genotype combinations and secondarily for examining stock center information on mutations and genes. The linkage map is being revised, and the list of genes and coordinates in the "Map" file represent the coordinates currently in the database. Access to the "external version" of the CGSC relational database itself is provided either as a menu option (#5) on this gopher (See sub menu item "How to Access..." and "CGSC Database") or by direct telnet to cgsc.biology.yale.edu To gain direct access, you must obtain a password and login as guest. Send e-mail to mary@cgsc.biology.yale.edu to obtain the current password for guest. --Using the CGSC Gopher v. Querying the CGSC Database-- The subset of information contained in the CGSC-gopher file can be accessed by text searches via IUWais. (See IUWAIS search features on the "2. CGSC Files and Database" menu.) You may also directly query the CGSC relational database. If your query is not easily formulated as a simple Boolean search or if a Wais search returns far too many instances to examine one by one, going to the form-based interface of the database will allow you to specify the query in a way that will limit the returns to those of most interest to you. The About file of the submenu provides examples of both types of queries. Read the DBAccess file on the submenu to learn how to navigate between data fields and menu bar and between object-forms. For more detailed instructions, contact Mary at address below for users' guide. Strains bearing CGSC numbers (the only strains present in the flat file) are available from the stock center. The stock center and the database development are supported by the National Science Foundation. For more information about the database or to request strains, contact Mary Berlyn at CGSC: mary@cgsc.biology.yale.edu Fungal Genetics This information source can be found under the following URL: http://kufacts.cc.ukans.edu/cwis/units/fgsc/main.html WWW server at Keck-IBT filamentous fungal databases URL: http://keck.tamu.edu/ibt.html or gopher://keck.tamu.edu Saccharomyces genomic information resource URL: http://genome-www.stanford.edu/ GenBank A searchable GenBank database can be accessed by addressing your gopher client to ftp.bio.indiana.edu, and looking under Genbank-Sequences/ GenomeNet GenomeNet is a Japanese computer network for genome research and related research areas in molecular and cellular biology. It can be accessed by WWW as: http://www.genome.ad.jp Microbiology The WWW Virtual Library >=Microbiology (Biosciences)<= is accessible at: http://golgi.harvard.edu/biopages/micro.html Protist Images Protist Image Data provides pictures and short descriptions of selected protist genera, especially those genera whose species are frequently used as experimental organisms or are important in studies of organismal evolution. The prerelease version of this database can be accessed through the WWW at the following URL: http://megasun.bch.umontreal.ca/protists/protists.html VII. MISCELLANEOUS -------------------- ACKNOWLEDGMENTS ---------------- This FAQ is adapted from a FAQ generated by Martin latterich, University of California, Berkeley. Thanks to Dave Kristofferson and the >=bionetters<= for compiling an excellent and comprehensive BIOSCI/bionet faq, which served as the basis to the technical subscription information in this MYCOLOGY FAQ in sections II. and III. Also many thanks to Keith Robinson, Harvard University, for much information on internet resources and Jim Graham, Indiana, for contributing Barbara Bachman's E. coli culture collection information. ------------------------------------------------------------------------ More information is always welcome. Please mail your suggestions,criticism and information to one of the following Discussion Leaders for bionet.mycology: tom@bio.tamu.edu leland@straylight.tamu.edu gsmay@bcm.tmc.edu Tom Adams Texas A&M University Leland Ellis Texas A&M University Greg May Baylor College of Medicine Tom Adams Department of Biology Texas A&M University College Station, TX 77843 409-845-1468 Tom@bio.tamu.edu From dgaunt at franklin.uhl.uiowa.edu Thu Oct 13 13:44:05 1994 From: dgaunt at franklin.uhl.uiowa.edu (Dennis Gaunt) Date: Mon Mar 7 09:15:36 2005 Subject: Help with culture please (newbie) References: Message-ID: <37jv5l$pqk@nexus.uiowa.edu> Jeffrey Ehlinger (an524@FreeNet.Carleton.CA) wrote: : My professor got some spores for Christmas this past year : and he asked me to start them for him. : The instructions were in Russian, so we first had to have them : translated. The species is "Boletus Edgilus" (the spelling could be wrong). : After two weeks, there is many white moldy looking patches on\ : the leaf mediumm. I have two trays that are being used. I have : lowered the moisture content on one of them to try to get rid of : the mold looking stuff. : I wonder is this the form the mycelium takes ? : Is that what I'm seeing.? : In two weeks, I will cut large holes in the plastic bag keeping the : moisture in, then hopefully I will get some results. : Please e-mail me if you have any idea on how to : culture Boletus edgilus. : -Jeff : PS- the medium should have been completely sterilized when I : autocl;aved it at 120C for twenty minutes. Do you mean Boletus edulis? Those white moldy looking patches are probably the fungus that you "planted". -- ---------------------------------------------------------------------- | Dennis D. Gaunt | Internet: dgaunt@uhl.uiowa.edu | | The University of Iowa | Voice: (319) 335-4500 | | Oakdale Research Campus | FAX: (319) 335-4555 | | Iowa City, Iowa 52242 | | ---------------------------------------------------------------------- From mb935645 at silver.shef.ac.uk Thu Oct 13 12:18:44 1994 From: mb935645 at silver.shef.ac.uk (D L Clarke) Date: Mon Mar 7 09:15:36 2005 Subject: DNA from dermatophytes Message-ID: <37jq5k$giu@hippo.shef.ac.uk> Does anyone know of a protocol for extraction of DNA from dermatophytes especially Trichophyton and Epidermophyton species. From cpullen at netcom15.netcom.com Thu Oct 13 22:46:00 1994 From: cpullen at netcom15.netcom.com (Chuck Pullen) Date: Mon Mar 7 09:15:36 2005 Subject: Carbon Monoxide and Fungi Message-ID: <84726.cpullen@netcom15.netcom.com> I am an industrial hygienist working on a project involving worker protection from carbon monoxide (CO) exposure during comercial fungi agriculture. We have found (CO) concentrations of up to 400 ppm during the growth cycle. CO concentrations correlate with Carbon Dioxide concentrations. Does anyone know a low tempreature biological sourse or process for the generation of CO? I initially thought that spontain. combustion of the compost was the sourse. However, the growth of the fungus is tempreature sensitive. The compost is not allowed to exceed 100 deg. F. which I would think should rule out simple oxidation as a CO sourse. Any help or referals would be appreciated. Chuck Pullen, CIH (cpullen@netcom.com) Pacific Safety Solutions - Sacramento Voice: (916) 687-7993 10215 Davis Road, Suite B Fax: (916) 687-8611 Wilton, CA 95693 "...to seek, to strive, to search, and not to yield." - - Tennyson From han at plantpath.wisc.edu Thu Oct 13 22:26:55 1994 From: han at plantpath.wisc.edu (Shinhan Shiu) Date: Mon Mar 7 09:15:36 2005 Subject: Sorry. Message-ID: In the previous message, it should be "not simply additive but synergistic". From han at plantpath.wisc.edu Thu Oct 13 22:21:54 1994 From: han at plantpath.wisc.edu (Shinhan Shiu) Date: Mon Mar 7 09:15:36 2005 Subject: Synergism, hard to define. Message-ID: I am a first-year grad. And I am going to study the mechanism of synergism between Verticillium dahliae (the potato-early-dying pathogen) and Pratylenchus penetrans (root lesion nematode). According the field data, yeild loss and symptoms tend to be severe when both pathogens are present in the same plot. What we and the other group observe is that the effect is simply additive but synergistic. Before I look into the mechanism of such synergistic effect, I would like to know if there is any well-defined system of synergistic interaction. Please indicate the information source if possible. And, I am tring to send some messages to Prof Carl H. Beckman, Univeristy of Rhode Island, who studies wilt disease of various plants. If anybody know his e-mail add, please inform me about that. Thank you. Shinhan Shiu han@plantpath.wisc.edu Department of Plant Pathology University of Wisconsin-Madison From mb927136 at silver.shef.ac.uk Fri Oct 14 09:48:44 1994 From: mb927136 at silver.shef.ac.uk (H Rolph) Date: Mon Mar 7 09:15:36 2005 Subject: DNA from dermatophytes Message-ID: <37m5oc$97e@hippo.shef.ac.uk> Does anyone know of a protocol for DNA extraction from dermatophytes (Trichophyton and Epidermatophyton) Helen Rolph H.J.Rolph@sheffield.ac.uk From rwk at sylvanres.com Fri Oct 14 06:52:41 1994 From: rwk at sylvanres.com (Richard W. Kerrigan) Date: Mon Mar 7 09:15:36 2005 Subject: Terminology, homokaryons, pedigrees Message-ID: <37lre9$q03@asia.lm.com> I'm looking for a concise term by which to refer to homokaryons in pedigrees. "The homokaryons that were crossed to produce the F1 hybrid xxx" is a bit cumbersome. I've recently used "progenitor homokaryons" but this does not seem completely satisfactory. More recently, I've seen the term 'midparents' used in this sense. I suspect the term is used by agricultural breeders but the several references I've consulted omit mention of this term. It seems clear and simple. Help, please: (1) What term(s) do you employ for this purpose? (2) What is known about origins and usage of 'midparents'; is it appropriate for this purpose? Thanks in advance. I'll be off-line until 14 November, so please reply or copy your reply to my e-mail address: rwk@sylvanres.com -- Rick Kerrigan Sylvan Research Cabot, PA USA From D.BRAYFORD at CABI.org Fri Oct 14 05:34:48 1994 From: D.BRAYFORD at CABI.org ("David Brayford ", IMI) Date: Mon Mar 7 09:15:37 2005 Subject: DNA from dermatophytes Message-ID: <2E9E5F64@smtp-gateway.cgnet.com> I've not actually worked on this, but happen to know of these references: Estruch, J. J., Antuna, C., Ferrer, S. & Ramon, D. (1989). Isolation of genomic DNA from Trichophyton mentagrophytes. Revista Iberica de Micologia 6: 62-66. Davison, F. D. & Mackenzie, D. W. R. ( 1984). DNA homology studies in the taxonomy of dermatophytes. Sabouraudia 22: 117-123. for mtDNA - see Suzuki et al., Mycopathologia 103: 147-151 (1988) and Nishio et al. Mycopathologia 117: 127-132 (1992) Dave Brayford International Mycological Institute UK ---------- From: BIOSCI-REQUEST To: mycology Subject: DNA from dermatophytes Date: 13 October 1994 5:18 Does anyone know of a protocol for extraction of DNA from dermatophytes especially Trichophyton and Epidermophyton species. From rwinder at PFC.Forestry.CA Thu Oct 13 11:22:39 1994 From: rwinder at PFC.Forestry.CA (Richard Winder) Date: Mon Mar 7 09:15:37 2005 Subject: Help with culture please (newbie) References: Message-ID: <1994Oct13.162239.18813@spruce.pfc.forestry.ca> In article , an524@FreeNet.Carleton.CA (Jeffrey Ehlinger) writes: > > My professor got some spores for Christmas this past year > and he asked me to start them for him. > The instructions were in Russian, so we first had to have them >translated. The species is "Boletus Edgilus" (the spelling could be wrong). Probaby Boletus edulis = cep = steinpilz, etc. = a fungus (basidiomycete) (this fungus forms symbiotic relationships with trees (mycorrhizae) and the sexual fruiting body is a large mushroom with pores, not gills. > After two weeks, there is many white moldy looking patches on\ >the leaf mediumm. I have two trays that are being used. I have >lowered the moisture content on one of them to try to get rid of >the mold looking stuff. > I wonder is this the form the mycelium takes ? Sounds right for a fungus. The mycelium is a mass of thread-like cells which represents the vegetative phase in the life cycle of many fungi. The B. edulis mycelium that I have cultured is white and cottony. Doesn't rule out the possibility of a contaminant, though. > Is that what I'm seeing.? >In two weeks, I will cut large holes in the plastic bag keeping the >moisture in, then hopefully I will get some results. > Please e-mail me if you have any idea on how to >culture Boletus edgilus. It would be nice if you could post the details of the Russian culture method. The only method I've seen reported in the literature involves the use of Hagem/Modess medium (see Pantidou, M. 1961. Cultural studies of Boletaceae. Gyrodon Merulioides and four species of Boletinus. Canadian Journal of Botany 39:1149-1162.) It also grows fairly well, at least at first, on malt extract agar amended with about .1% (or so) yeast extract. I imagine you need a pretty big petri dish to get a fruiting body though :-) -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From koenigg at rnisd0.DNET.roche.com Fri Oct 14 10:46:55 1994 From: koenigg at rnisd0.DNET.roche.com (koenigg@rnisd0.DNET.roche.com) Date: Mon Mar 7 09:15:37 2005 Subject: PFGE separation of mt DNA? Message-ID: <9410141543.AA01620@mailgate.roche.com> Does anyone have a protocol for pulsed field electrophoretic separation of fungal mitochondrial DNA? We are studying the chromosomal arrangements of the human pathogens Coccidioidesimmitis and Histoplasma capsulatum. We would like to apply the right PFGE conditions to separate the mt DNA from the chromosomes. I'd appreciate any references. Thank you. Gina Koenig Roche Molecular Systems Koenigg@rnisd0.dnet.roche.com (510)-814-2815 fax (510) 522-1285 1145 Atlantic Avenue Alameda, CA 94501 USA From an524 at FreeNet.Carleton.CA Fri Oct 14 00:59:37 1994 From: an524 at FreeNet.Carleton.CA (Jeffrey Ehlinger) Date: Mon Mar 7 09:15:37 2005 Subject: Help with culture please (newbie) Message-ID: I will look at the patches of moldy -looking stuff. I will look at it under a microscope and look for sporulation. Maybe I can identify it as a beginning of the Boletus edulis or perhaps just penicillin. :( -Jeff PS_ Thanks for all the help folks. From geomay at MOLBIO.CBS.UMN.EDU Fri Oct 14 13:02:44 1994 From: geomay at MOLBIO.CBS.UMN.EDU (Georgiana May) Date: Mon Mar 7 09:15:37 2005 Subject: midparents Message-ID: <9410141800.AA17870@molbio.cbs.umn.edu> Rick, I think we should seek to adopt more standard genetic terminology for our crossing experiments. The problem, as I see it, is that we are NOT crossing parents, spores are gametes, the dikaron from which they come are the parents in the sense that breeders use. We just have long-lived gametes which are not available to plant and animal breeders. So, could one scan the literature for what is done in other haploid/diploid systems? What do the yeast breeders (for beer or wine?) do? My only strong feeling is that we shouldn't be calling them parents but probably a lot of people will disagree. Georgiana From jweiland at BADLANDS.NODAK.EDU Sat Oct 15 11:41:34 1994 From: jweiland at BADLANDS.NODAK.EDU (John J Weiland) Date: Mon Mar 7 09:15:37 2005 Subject: EMBO workshop Filamentous Fungi Message-ID: Can anyone relay information to me about the 1995 EMBO workshop on Filamentous Fungi (if it is in 1995)? Where will it be and when? What is the due date for abstracts and how much is registration? THanks! John Weiland jweiland@badlands.nodak.edu From jweiland at BADLANDS.NODAK.EDU Sat Oct 15 10:41:53 1994 From: jweiland at BADLANDS.NODAK.EDU (John J Weiland) Date: Mon Mar 7 09:15:37 2005 Subject: Manipulations with Filamentous Fungi Message-ID: I'm kind of new to these beasts, and am looking for some input into the following questions. The fungus I'm working with is a pathogen of barley called Pyrenophora teres. 1) We currently store long-term cultures of the fungus on silica gel. Is this a standard long term storage media? Can spores be stored in glycerol at -80 degrees C. Can they be stored as stabs in agar (like many bacterial isolates)? 2) Conidia of P. teres can be collected from mycelia grown in culture. Ascospores, however, result from the mating of two opposite mating types of the fungus on barley plants (but not in culture). Has anyone seen mention of mutations in the fungus, or culture conditions, that may induce productive mating of such a fungus in culture, rather than on its normal host plant? 3) Any papers out there on the "bulk segregation analysis" of traits in haploid fungi using PCR? Did Michelmore's group do it with the fungus, or just with the plant traits? Thanks in advance! John Weiland jweiland@badlands.nodak.edu From stamets1 at aol.com Sat Oct 15 14:33:01 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:37 2005 Subject: ATCC & Psilocybe References: Message-ID: <37papd$s10@newsbf01.news.aol.com> In article , harris@bhc.com (Bob Harris) writes: Bob is right.The compounds are illegal; not the genus or its members. Of interest to many of you, I have finally got around to submitting a new species for publication - Psilocybe azurescens which I have co-authored with Jochen Gartz who did extensive analyses. A surprising finding is that when the mycelium of this species, otherwise extremely potent, was cultured on 10% plus malt agar medium (100 grams/liter), there were NO detectable amounts of psilocybin, psilocin, and/or baeocystin! In contrast, mycelia on 2%MEA had, collectively, more than 2.5% (d.w.) of the same. Bizarre, but true.This is the third species I have named and it is by far, the most interesting. It may well be the most potent species ever analyzed. This work was done under the auspices of a DEA license through Dr. Michael Beug of The Evergreen State College and has no connection to Fungi Perfecti. I agree that the loss of these cultures is a loss to us all - especially for future works. paul stamets The article will be published in Integration. The ms. was 20 pages and should clear up or - in the minds of some - further complicate the taxonomy of the Caerulescent species. (No cultures are available. Please do not ask. The type location is Astoria, Oregon.) From stamets1 at aol.com Sat Oct 15 14:23:04 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:37 2005 Subject: Kampucha References: <36v1lf$bva@usenet.rpi.edu> Message-ID: <37pa6o$rsa@newsbf01.news.aol.com> In article <36v1lf$bva@usenet.rpi.edu>, bermaa@vccsouth12.its.rpi.edu (Adam Louis Berman) writes: I am writing an article for Mushroom, the Journal on Kombucha. I have a fair amount of information - some credible, some quite incredible. Give me your mailing address and I will send you info. on the compounds generated by it. paul stamets From mb927136 at silver.shef.ac.uk Sun Oct 16 07:22:17 1994 From: mb927136 at silver.shef.ac.uk (H Rolph) Date: Mon Mar 7 09:15:37 2005 Subject: h.j.rolph@sheffield.ac.uk Message-ID: <37r5tp$n6e@hippo.shef.ac.uk> dna from dermatophytes responses From aeiham at waikato.ac.nz Sat Oct 15 19:44:58 1994 From: aeiham at waikato.ac.nz (aeiham@waikato.ac.nz) Date: Mon Mar 7 09:15:37 2005 Subject: Q: soil fungi Peniophora & Pleurotus Message-ID: <1994Oct16.134458.34211@waikato.ac.nz> Can anyone tell me off the top of there head what type of soil fungi _Peniophora gigantea_ and _Pleurotus ostreatus_ are? Just a general description like ecto or VAM mycorrhiza or fungal antagonist similar to Trichoderma or whatever. Many thanks, Terry Brown. From bpretto at io.org Sun Oct 16 08:38:21 1994 From: bpretto at io.org (Bruno Pretto & Paula Vopni) Date: Mon Mar 7 09:15:37 2005 Subject: fungus tea Message-ID: Does anybody have any information on verified cases of adverse reactions to consumption of Kombucha tea? Bruno Pretto From bmtyler at UCDAVIS.EDU Mon Oct 17 10:02:28 1994 From: bmtyler at UCDAVIS.EDU (Brett Tyler) Date: Mon Mar 7 09:15:37 2005 Subject: Manipulations with Filamentous Fungi Message-ID: <199410171502.IAA14033@bullwinkle.ucdavis.edu> At 8:41 AM 10/15/94 -0700, John J Weiland wrote: >I'm kind of new to these beasts, and am looking for some input into the >following questions. The fungus I'm working with is a pathogen of barley >called Pyrenophora teres. > >1) We currently store long-term cultures of the fungus on silica >gel. Is this a standard long term storage media? Can spores be stored >in glycerol at -80 degrees C. Can they be stored as stabs in agar (like >many bacterial isolates)? > >2) Conidia of P. teres can be collected from mycelia grown in >culture. Ascospores, however, result from the mating of two opposite >mating types of the fungus on barley plants (but not in culture). Has >anyone seen mention of mutations in the fungus, or culture conditions, >that may induce productive mating of such a fungus in culture, rather >than on its normal host plant? > >3) Any papers out there on the "bulk segregation analysis" of traits >in haploid fungi using PCR? Did Michelmore's group do it with the >fungus, or just with the plant traits? > >Thanks in advance! > >John Weiland jweiland@badlands.nodak.edu Regarding 1) In our experience with Neurospora, silica gel is in fact the best long term storage method. Slants or glycerol can be used, depending on the fungus, but can be unreliable. Liquid nitrogen storage is another excellent long term storage method. Regarding 3) We are using bulked segregant analysis for Phytophthora sojae. There is no reason at all why this method can't be used on any genetic progeny showing random segregation of genes. In haploids, BSA should work even better than diploids because dominance and recessiveness (especially of the RAPDs themselves) is not a problem. The most important considerations for BSA are (i) that the trait of interest is determined by a single locus, (ii) that you have at least 8-10 progeny for each bulk (you should have much more than this if the genetics have been done well), (iii) that you are absolutely sure that none of the chosen progeny have been mis-scored and (iv) that there is a sufficient level of DNA sequence polymorphism between the parents (at least 5% of all RAPD bands should differ between the two parents, otherwise you will have a real struggle). Can't help with 2). **************** Brett Tyler Professor bmtyler@ucdavis.edu **************** From stamets1 at aol.com Mon Oct 17 09:38:03 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:37 2005 Subject: fungus tea References: Message-ID: <37u28b$mof@newsbf01.news.aol.com> In article , bpretto@io.org (Bruno Pretto & Paula Vopni) writes: Bruno- I have heard that 5 people in Israel died from drinking a contaminated batch of tea. Also, there is a report from Florida which I am tracking down where a woman allegedly died from drinking Kombucha Tea. I have finished writing an extensive article on Kombucha for Mushroom, the Journal which will appear in the next issue. If you can't wait, I'll be glad to send a copy of the ms. (sans photos). How's your cultivation projects? pablo-san From mycoworld at aol.com Mon Oct 17 10:54:02 1994 From: mycoworld at aol.com (MycoWorld) Date: Mon Mar 7 09:15:37 2005 Subject: Q: soil fungi Peniophora & Pleurotus Message-ID: <37u6mq$nqa@newsbf01.news.aol.com> >aeiham@waikato.ac.nz Writes: >Can anyone tell me off the top of there head what type of soil fungi >_Peniophora gigantea_ and _Pleurotus ostreatus_ are? Pleurotus ostreatus is commonly known as the oyster mushroom. It's widely cutivated - by last report #2 in world production after Agaricus. It's a decayer- grows on tress, straw or just about anything. Peniophora gigantea took me to David Arora's book "Mushrooms Demystified". It's sort of a bracket fungus forming a "paper-thin crust on dead conifers". Apparently a decayer also. Jerry Haugen, The Mushroom Growers' Newsletter MycoWorld@aol.com From FNM at GNV.IFAS.UFL.EDU Mon Oct 17 10:49:40 1994 From: FNM at GNV.IFAS.UFL.EDU (FNM@GNV.IFAS.UFL.EDU) Date: Mon Mar 7 09:15:37 2005 Subject: PFGE of mtDNA Message-ID: <01HIDRD49ZMQ8XOKGW@gnv.ifas.ufl.edu> Dear Gina, In response to your inquiry about PFGE of mtDNA, you might find the following references helpful. Fukuhara H, Sor F, Drissi R, Dinouel N, Miyakawa I, Rousset S, Viola A-M (1993) Linear mitochondrial DNAs of yeasts: Frequency of occurrence and general features. Mol Cell Biol 13:2309-2314 Han Z, Stachow C (1994) Analysis of Schizosaccharomyces pombe mitochondrial DNA replication by two dimensional gel electrophoresis. Chromosoma 103:162-170 Maleszka R (1993) Single stranded regions in yeast mitochondrial DNA revealed by pulsed field electrophoresis. App Theor Electroph 3:259-263 Maleszka R (1992) Electrophoretic profiles of mitochondrial plasmids in Neurospora suggest they replicate by a rolling circle mechanism. Biochem Biophys Res Commun 186:1669-1673 Maleszka R, Clark-Walker GD (1992) In vivo conformation of mitochondrial DNA in fungi and zoosporic moulds. Curr Genet 22:341-344 Maleszka R, Skelly PJ, Clark-Walker GD (1991) Rolling circle replication of DNA in yeast mitochondria. J EMBO 10:3923-3929 Sincerely, Frank Martin  From KSROSTAND at bmg.bhs.uab.edu Mon Oct 17 14:33:57 1994 From: KSROSTAND at bmg.bhs.uab.edu (KSROSTAND@bmg.bhs.uab.edu) Date: Mon Mar 7 09:15:37 2005 Subject: yeast adherence Message-ID: Does anyone know a way to block non-specific binding of Candida albicans to plastic? I have tried coating the plastic with albumin, gelatin, and milk proteins(skim milk) but they don't help. I know the yeast does not stick to glass but once the glass is exposed to fetal calf serum ( which it will be in my experiements) the yeast are quite happy to adhere. Thanks. Kathy Rostand, Ph.D. Department of Biochemistry and Molecular Genetics University of Alabama in Birmingham Birmingham, AL 35294 TEL: (205) 934-1967 FAX: (205) 975-2547 e-mail: ksrostand@bmg.bhs.uab.edu From Unknown Mon Oct 17 12:57:10 1994 From: Unknown (Unknown) Date: Mon Mar 7 09:15:37 2005 Subject: Information about mushroom(Armillaria caligata) Message-ID: <37udtm$g2o@darwin.nbnet.nb.ca> I am looking for any information concerning the mushroom Armillaria caligata. I am particularly interested in the techniques involved in growing this mushroom. Thanks in advance Robert Mullin From REDHEADS at NCCCOT.AGR.CA Mon Oct 17 15:35:33 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:37 2005 Subject: Armillaria caligata (R. Mullin) Message-ID: <01HIE1BXDGJ6002IAT@GW.AGR.CA> RE Armillaria caligata Armillaria caligata is really Tricholoma caligatum. It is mycorrhizal and sometimes is said to be a synonym of the Japanese Matsutake, a highly prized edible. Presumably interest stems from that aspect. For those accepting the synonymy there is Japanese literature on attempts to cultivate the mycorrhizal fungus (not necessarily the mushroom fruitbodies). For the true T. caligatum there is less literature. Just what is that you want to know? From griffin at gamera.syr.edu Mon Oct 17 08:14:24 1994 From: griffin at gamera.syr.edu (David H. Griffin) Date: Mon Mar 7 09:15:37 2005 Subject: midparents Message-ID: <37ttbg$93@newstand.syr.edu> From stamets1 at aol.com Mon Oct 17 19:08:01 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:37 2005 Subject: yeast adherence References: Message-ID: <37v3l1$3m6@newsbf01.news.aol.com> In article , KSROSTAND@bmg.bhs.uab.edu writes: Re: Concerning the prevention of yeasts bonding to plastic: Have you tried silicon spray? paul stamets From A.F.Brown at bradford.ac.uk Tue Oct 18 13:22:06 1994 From: A.F.Brown at bradford.ac.uk (Fred) Date: Mon Mar 7 09:15:37 2005 Subject: palaeoethnomycology Q's Message-ID: I am currently wanting to investigate the use of psychoactive fungi in archaic cultures. Any opinions, leads, references, recipes greatly appreciated. I am not another spaced-out hippy with my head in a cloud (of pot smoke), I am trying to understand what has been taboo for a long long time in this culture, and eventually piece together what little research there is in this area. Does Terence Mckenna ring any bells ?.... thanks FRED xxx From D.BRAYFORD at CABI.org Tue Oct 18 06:23:43 1994 From: D.BRAYFORD at CABI.org ("David Brayford ", IMI) Date: Mon Mar 7 09:15:37 2005 Subject: Manipulations with Filamentous Fungi Message-ID: <2EA3B0DF@smtp-gateway.cgnet.com> 1. Silica gel is fine for, say, 5 years if you keep it refrigerated. Liquid nitrogen or lyophilisation are better, but obviously more expensive. It is not recommended to keep them growing on agar since they are likely to mutate. 2. I don't know a synthetic medium for crossing this fungus. However, a good ploy is to mimic the natural conditions by pairing isolates on bits of autoclaved barley straw (say 2-3 cm lengths) placed on plain agar. Allow the isolates to grow for 2 weeks to allow colonisation of the straw, then transfer to cool conditions (10 C) for 2-3 weeks, then transfer to 20-25 C in alternating dark and daylight. The fungus usually forms its sexual state in spring after a cold winter snap - so the cool incubation period may help promote perithecial formation - it certainly does in other fungi. Dave Brayford International Mycological Institute d.brayford@cabi.org ---------- From: BIOSCI-REQUEST To: mycology Subject: Manipulations with Filamentous Fungi Date: 15 October 1994 8:41 I'm kind of new to these beasts, and am looking for some input into the following questions. The fungus I'm working with is a pathogen of barley called Pyrenophora teres. 1) We currently store long-term cultures of the fungus on silica gel. Is this a standard long term storage media? Can spores be stored in glycerol at -80 degrees C. Can they be stored as stabs in agar (like many bacterial isolates)? 2) Conidia of P. teres can be collected from mycelia grown in culture. Ascospores, however, result from the mating of two opposite mating types of the fungus on barley plants (but not in culture). Has anyone seen mention of mutations in the fungus, or culture conditions, that may induce productive mating of such a fungus in culture, rather than on its normal host plant? 3) Any papers out there on the "bulk segregation analysis" of traits in haploid fungi using PCR? Did Michelmore's group do it with the fungus, or just with the plant traits? Thanks in advance! John Weiland jweiland@badlands.nodak.edu From rarnold at teleport.com Mon Oct 17 23:49:12 1994 From: rarnold at teleport.com (Ralph D. Arnold) Date: Mon Mar 7 09:15:38 2005 Subject: A: Check This Out; B: Help Please Message-ID: <91454.rarnold@teleport.com> A: Check This Out: I'm setting up a gopherspace designed to help mushroom cultivators - both hobby and comm'l. Within this gopher is a partial list of commercial sources with internet/email access (major requirement); small list of internet resources (still compiling); files on growing shiitakes (from Mushroompeople) + max. use of autoclaves & cultivating lion's mane (from Mushroom Growers' Newsletter) + excerpts dealing with cultivating fungi from various sources/newsletters. Access to this gopherspace: Via WWW gopher://gopher.teleport.com/11/users/rarnold Via Gopher gopher.teleport.com : select "Users"; then "ON-LINE ACCESS"; then "PLANTS_BY_EMAIL"; then "MUSHROOMS, FUNGI, MYCOLOGY" (This gopherspace is changing almost daily) BTW - if you don't have gopher-ability and you would like to review the material I have collected, simply send me an email and ask for one or more of these subjects (each will be sent as a separate email): Commercial Sources..... Shiitake Article (by MUSHROOMPEOPLE)....... Articles on Autoclave and Cultivating Lion's Mane (by Mushroom Growers'Newsletter)........ EXCERPTS (from various sources)....... FREE Internet Email Materials/Sources B: Help Please : Do you know of commercial sources I might add (they must have internet/email access)? Do you have some text files I might add \ Please send these to me OR some newsletter articles from your \ as part of email message - society I might excerpt THAT deal with / I have trouble with cultivating wild/gourmet fungi ?? / receiving enclosures/files What other suggestions might you have to improve this info-source? Ralph D. Arnold rarnold@teleport.com P.O. Box 889, Oregon City, OR 97045 maintainer of "ON-LINE ACCESS" gopherspace -encouraging internet access gopher://gopher.teleport.com/11/users/rarnold From bpretto at bonk.io.org Tue Oct 18 07:16:28 1994 From: bpretto at bonk.io.org (bruno pretto) Date: Mon Mar 7 09:15:38 2005 Subject: medicinal fungi Message-ID: <380eat$kc1@ionews.io.org> Does anybody have any information on the use of Inonotus obliquus (Fr.) Pilat, as a medicinal tea. This is a hard polypore and apparently has been used by the Russian community as a medicinal tea for centuries. -- Bruno Pretto. NAMA Trustee. Mycological Society of Toronto. Amateur mycologist. Secretary. Oak Forest Mushroom Association (outdoor shiitake) Paula Vopni. Environmental Research. From REDHEADS at NCCCOT.AGR.CA Tue Oct 18 13:54:07 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:38 2005 Subject: Edible & Poisonous Mushrooms of Canada Message-ID: <01HIFC1R96JM002U1I@GW.AGR.CA> Excuse me if I am breaking any unwritten rules (and please let me know [politely] if I am as this is all new to me). We have several new copies of "Edible and poisonous mushrooms of Canada" 326 pgs. by J.W. Groves, 1981 printing, available for exchange only, one per person. Is there any interest? Scott Redhead From mycoworld at aol.com Tue Oct 18 22:28:14 1994 From: mycoworld at aol.com (MycoWorld) Date: Mon Mar 7 09:15:38 2005 Subject: Q: Cortinellus shiitake Message-ID: <3823oe$h69@newsbf01.news.aol.com> Is "Cortinellus shiitake" (from a 1979 reference) the same beast as "Lentinula edodes"? Thanks for any feedback. Jerry Haugen, The Mushroom Growrs' Newsletter Mycoworld@aol.com From mab20 at cornell.edu Wed Oct 19 08:48:47 1994 From: mab20 at cornell.edu (Michael Anthony Ball) Date: Mon Mar 7 09:15:38 2005 Subject: DMSO Message-ID: Greetings, Can anyone help with references regarding the anti-fungal properities of DMSO?? Is there any data out there that supports its use as an anti-fungal and if so are there any reported MIC values?? Thank-you, M.A. Ball, DVM Department Clinical Science College Veterinary Medicine Cornell University From REDHEADS at NCCCOT.AGR.CA Wed Oct 19 12:32:13 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:38 2005 Subject: Armillaria caligata (R. Mullin) Message-ID: <01HIGNDBVHOI0031HF@GW.AGR.CA> When I add all your questions up they tell me you are interested in harvesting the Matsutake commercially, and that you are starting from scratch. In North America you should be interested in Tricholoma magnivelare (a.k.a. Armillaria ponderosa, Tricholoma ponderosum), the North American Matsutake (a.k.a. the Pine Mushroom). You are therefore not interested in cultivating sterile mycelium. The Pine Mushroom is not overly common in New Brunswick but probably can be found on well drained sandy or gravelly soils under pines (and possibly hemlocks). If you find some let me know. Researching the literature could be a major task. I suggest you go to a research library and ask to use Biosis or some other source. Again I must ask what is it you are ultimately attempting to do and where are you starting? From R.Mullin at nbnet.nb.ca Wed Oct 19 10:00:53 1994 From: R.Mullin at nbnet.nb.ca (Robert Mullin) Date: Mon Mar 7 09:15:38 2005 Subject: Armillaria caligata (R. Mullin) References: <01HIE1BXDGJ6002IAT@GW.AGR.CA> Message-ID: <383cb5$c2d@darwin.nbnet.nb.ca> In article <01HIE1BXDGJ6002IAT@GW.AGR.CA>, REDHEADS@NCCCOT.AGR.CA says: > >RE Armillaria caligata > >Armillaria caligata is really Tricholoma caligatum. It is >mycorrhizal and sometimes is said to be a synonym of the >Japanese Matsutake, a highly prized edible. Presumably >interest stems from that aspect. For those accepting the >synonymy there is Japanese literature on attempts to >cultivate the mycorrhizal fungus (not necessarily the >mushroom fruitbodies). For the true T. caligatum there is >less literature. Just what is that you want to know? Thank you for your reply. I am interested in the following: What is the difference between T. caligatum and the Japanese Matsutake? How would I get the Japanese literature? How would I cultivate T. caligatum? What conditions and habitat produce optimum growth of T. caligatum? Where would I find T. caligatum in New Brunswick? Your reply would be greatly appreciated. R. Mullin From Moulliard at mbla.ucl.ac.be Wed Oct 19 09:53:02 1994 From: Moulliard at mbla.ucl.ac.be (Moulliard) Date: Mon Mar 7 09:15:38 2005 Subject: MUCL vcancy Message-ID: > FACULTE DES SCIENCES AGRONOMIQUES > UNIVERSITE CATHOLIQUE DE LOUVAIN (UCL) > Louvain-la-Neuve, Belgium. > > At the College of Agriculture of the Catholic University of Louvain, a full > time faculty position has become available: > Field: Molecular biology of microorganisms > Aim: to develop a research group devoted to molecular biology applied to > fungi and bacteria and to supervise the mycotheque of the university > (23 000 strains) with the development and use of molecular biology > techniques applied to taxonomy. > Teaching will be in the areas of microbiology (mycology) and molecular > biology. > Special qualifications: publications in molecular biology applied to > microorganisms; a knowledge of fungal taxonomy will be an asset; otherwise, > it will have to be acquired. > Duties will include lectures and practical tuition, in French, to > undergraduate students, and supervision of postgraduate students. > Candidates should have a Ph.D. in the field, postdoctoral experience and > internationnally recognised publications. They should be well recognised > scientists with a broad knowledge of the various aspects of the field. > They should have capability for teaching and for research group leadership. > The recruitment level will depend upon experience and qualifications. > Starting date will be October 1, 1995. > The application should include a curriculum vitae, a list of publications > and reprints of the 5 most representative ones, a research project for 5 > years, three letters of reference and, if possible, the name of two belgian > scientific personalities, not members of the UCL, to whom information could > be asked. It should be sent before November 15th, 1994, to: > Professeur P. MACQ, Recteur de l'Université Catholique de Louvain, Halles > universitaires, Place de l'Université 1 à B-1348 LOUVAIN-LA-NEUVE, Belgium. > > Inquiries may be directed to : > Professeur E. Persoons, Doyen de la faculté des Sciences agronomiques de > l'Université catholique de Louvain, place Croix du Sud 2, bte 1 at 1348 > Louvain-la-Neuve, Belgium. > Tél.: 32.10.47.37.19 - Fax: 32.10.47.47.45 - e-mail: > Persoons@sagr.ucl.ac.be -- Moulliard Charles Mycotheque de l'Universite Catholique de Louvain (MUCL) Place Croix du Sud, 3 B-1348 Belgium Tel.: +32-(10)-47.37.37 Fax.:+32-(10)-45.15.01 Email: Moulliard@mbla.ucl.ac.be From kzeller at BILBO.BIO.PURDUE.EDU Wed Oct 19 16:52:34 1994 From: kzeller at BILBO.BIO.PURDUE.EDU (kzeller@BILBO.BIO.PURDUE.EDU) Date: Mon Mar 7 09:15:38 2005 Subject: Looking For a PostDoc or Research Position: Message-ID: <199410192151.OAA08033@net.bio.net> Looking For a PostDoc Position or Research Position: To those insterested in considering, I am a Ph.D. candidate in the Population Biology and Ecology area of the school of Biological sciences at Purdue University, and expect to defend my thesis before the end of 1994. My thesis concerns determining the number of distinctive forms within the obligately parasitic, plant pathogenic powdery mildew Erysiphe cichoracearum, and the extent and evolution of their host specificity. I have primarily used RFLP information from PCR amplified regions of the genomic ribosomal genes to characterize the diversity within this widespread fungal species. During my tenure in the laboratory of Dr. Morris Levy I have also worked on population biology and pathotypic evolution in the rice blast fungus (Pyricularia grisea). I have experience with standard aseptic procedures and microscopic techniques, southern blotting and probing, amplification of RAPDs, and have experience using a number of phylogenetic inference software packages including versions of PAUP, MacClade, Phylip, and DNA Sequence alignment software. My research interests are broad within the areas of understanding evolutionary processes, molecular genetics, and in phylogenetic reconstruction, but I am especially interested in studies of interactions between hosts and their symbionts. I am looking for post-doctoral or research associate positions, particularly in the areas of plant, fungal or microbial molecular genetics and/or systematics. I will be available for positions beginning in or after the summer/autumn of 1995. If you have a post-doctoral or research position to offer, please do not hesitate to contact me at. Kurt A. Zeller Dept. of Biological Sciences Purdue University, West Lafayette, IN 47906-1392 (USA) Internet: Phone: (317) 497-3596 Fax: (317) 494-0876 Attached is a copy of my CV (as of 9/94). I thank you in advance for your consideration. ----------------------------------------------------------- KURT A. ZELLER Vita PERSONAL: Born: April 12, 1965, LaPorte, Indiana (USA Citizen) Present Address: Lilly Hall of Life Sciences Department of Biology, Purdue University West Lafayette, Indiana 47907-1392 Internet: Phone: Office: (317) 494-8133, Home: (317) 497-3596 Fax: (317) 494-0876 EDUCATIONAL BACKGROUND: B.S. in Biology, College of Arts and Sciences, 1987 Indiana University, Bloomington, Indiana PhD. Population Biology, Department of Biological Sciences, 1994 Purdue University, West Lafayette, Indiana (Pending) MAJOR RESEARCH INTERESTS: Host-Parasite Coevolution and Cospeciation, Molecular Systematics, Molecular Genetics, and Evolution in Symbiotic Associations. PROFESSIONAL EXPERIENCE: Research Assistant -- In projects involving the use of molecular techniques to study population biology and phylogenetic relationships in fungal plant pathogens, especially Erysiphe cichoracearum and Magnaporthe grisea, Purdue University, for Dr. Morris Levy, Spring 1990 to Present. Graduate Instructor --Systematic Biology, Purdue University, Fall 1994. Evolution, Purdue University, Spring 1992. Diversity, Ecology and Behavior, Introductory Biology Laboratory, Purdue University, Fall 1989, 1990 and 1991. Development, Structure and Function of Organisms, Introductory Biology Laboratory, Purdue University, Spring 1989 and Spring 1990. Laboratory Assistant -- Involved in projects studying the effects of endophytic fungi of grasses on insect herbivory and in influencing the ability of infected hosts in withstanding environmental stresses, Indiana University, for Dr Keith Clay, Fall 1986 -- Fall 1987. PROFESIONAL SOCIETY AFFILIATIONS: Member -- American Society of Plant Taxonomists since 1991. Member -- Indiana Academy of Science since 1991. Member -- Mycological Society of America since 1993. AWARDS: 1991: Sigma Xi Grant-in-Aid of Research for a study titled, "Systematics and Evolution of Host-Specificity in Erysiphe cichoracearum". 1992-94: Purdue Research Foundation Graduate Student Fellowship, for a study titled, "Systematics and Evolution of Host-Specificity in the Plant Pathogen Erysiphe cichoracearum". 1994: Mycological Society of America Mentor Travel Grant to the IMC5,to present a poster titled, "The Evolution of Host Specificity in the Obligate Plant Parasite Erysiphe cichoracearum: A Comparative Phylogenetic Examination". 1994: Mycological Society of America Graduate Research Prize for a poster titled, The Evolution of Host Specificity in the Obligate Plant Parasite Erysiphe cichoracearum: A Comparative Phylogenetic Examination". ABSTRACTS AND PRESENTATIONS : Zeller, K. A. and M. Levy. 1991. Molecular Techniques for Systematic Studies of Powdery Mildew Phytopathogens: 11th Midwest Ecology and Evolution Conference (MEEC). Lexington KY, March 15-17, (abstract). Zeller, K. A. and M. Levy. 1991. DNA Mini-preparation for Magnaporthe Field Isolates. 5th Annual Meeting of the Rockefeller Foundation International Program on Rice Biotechnology. Offprint, 4 pages. Zeller, K. A. 1993. Form Species Differentiation Within Erysiphe cichoracearum Revealed by RFLP's. 13th Midwest Ecology and Evolution Conference (MEEC). West Lafayette IN, March 12-14, (abstract). Zeller, K.A. and M. Levy. 1993. Parasite/Host Coevolution in the Powdery Mildew Erysiphe cichoracearum: A Comparative Phylogenetic Examination. 1993 Fall Meeting of the Indiana Academy of Sciences (IAS). West Lafayette, IN, November 4-5, (abstract). Zeller, K.A. and M. Levy. 1994. The Evolution of Host Specificity in the Obligate Plant Parasite Erysiphe cichoracearum: A Comparative Phylogenetic Examination. 5th International Mycological Congress (IMC5). Vancouver, B.C. (Canada), August 14-21, (abstract). PUBLICATIONS: C. R. Casela, A. S. Ferreira, K. A. Zeller, and M. Levy. Pathotype Variation in the Sorghum Anthracnose Fungus: A Phylogenetic Perspective. In, Disease Management Through Biotechnology, (R. A. Frederiksen and J. F. Leslie eds). (in press). Zeller, K. A. and M. Levy. Intra-species Differentiation in the Powdery Mildew Erysiphe cichoracearum Determined With rDNA RFLPs. Molecular Ecology (in review). Zeller, K. A. Phylogenetic Relatedness Within the Genus Erysiphe Estimated With Morphological Characteristics. Mycologia (in review). Zeller, K. A. and M. Levy. Parasite - Host Coevolution Among Erysiphe spp. at Host Familial Levels. Evolution. (in preparation). References: References are available upon request. --------------------------------------------- From staben at seqanal.mi.uky.edu Thu Oct 20 16:37:03 1994 From: staben at seqanal.mi.uky.edu (Karen Marionneaux) Date: Mon Mar 7 09:15:38 2005 Subject: Neurospora genome project Message-ID: <386ntv$10f@s.ms.uky.edu> I wanted to check on progress in coordinating and accumulating genomic mapping information for Neurospora. I would also like to know whether a discussion of this topic is planned for the Asilomar meeting, or whether a discussion of fungal genome mapping (which fungi, how, who is responsible) should be planned. I would be happy to post a summary of responses or to see the issue discussed in this forum. From dje0282 at SUMMA.TAMU.EDU Thu Oct 20 07:54:06 1994 From: dje0282 at SUMMA.TAMU.EDU (Dan Ebbole) Date: Mon Mar 7 09:15:38 2005 Subject: Neurospora mapping Message-ID: <199410201254.FAA21184@net.bio.net> I would like to know the status of genome mapping information being collected for Neurospora. Two years ago this topic was discussed at Asilomar, and with the meeting coming up again, I thought it might be of interest to discuss plans for fungal genome mapping in general and Neurospora in particular. The YAC library is available from the FGSC and I just got a copy of it. I suspect that a systematic physical map is a long way off based on the progress that has been made so far. I imagine people will eventually get hybridization filters made and find which YAC contain genes or RFLP markers they are interested in. Maybe it would be useful for FGSC to also provide the YAC library as DNA fixed to membranes for hybridization. It would probably save money to pay FGSC for the membranes rather than have every lab make their own. ------------------------ Dr. Daniel Ebbole dje0282@summa.tamu.edu Dept. of Plant Pathology and Microbiology Texas A&M University College Station, TX 77843-2132 From staben at seqanal.mi.uky.edu Wed Oct 19 19:20:23 1994 From: staben at seqanal.mi.uky.edu (Chuck Staben) Date: Mon Mar 7 09:15:38 2005 Subject: Neurospora genome project Message-ID: <384d47$1l5@s.ms.uky.edu> I would like to know the status of genome mapping information being collected for Neurospora. Two years ago this topic was discussed at Asilomar, and with the meeting coming up again, I thought it might be of interest to discuss plans for fungal genome mapping in general and Neurospora in particular. From ptm at MOLBIO.CBS.UMN.EDU Thu Oct 20 10:01:25 1994 From: ptm at MOLBIO.CBS.UMN.EDU (Paul T. Magee) Date: Mon Mar 7 09:15:38 2005 Subject: fungal genome mapping Message-ID: <9410201457.AA09179@molbio.cbs.umn.edu> Genomic mapping of Candida albicans is underway. We have assigned specific probes to a library of fosmids (cosmids with an F' origin). We now have iden- tified fosmids amounting to about 10% of the genome and have a contig of about 150kb on one chromosome. (All the probes are assigned to the karyotype as well as to the fosmid library.) We keep our map up to date on an almost daily basis on mosaic. To look at how we have organized the data, you can look at hhtp://alces.med.umn.edu/start.html on the WWW or you can get a non-imaged ver- sion on the U of Minnesota Gopher, looking for Candida. The object of the mosaic server is to allow people to make daily and convenient use of the latest data. If you want any more information, email me or Stew Scherer (who knows much more about the server than I do) at stew@lenti.med.umn.edu. Pete Magee (ptm@molbio.cbs.umn.edu) From stamets1 at aol.com Fri Oct 21 12:03:02 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:38 2005 Subject: More tea fungi References: Message-ID: <388s86$iq3@newsbf01.news.aol.com> In article , mdw3@delphi.com writes: Sounds like you have Kombucha, aka the Manchurian Mushroom or the Tea Fungus. paul stamets From stamets1 at aol.com Fri Oct 21 12:01:04 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:38 2005 Subject: medicinal fungi References: Message-ID: <388s4g$ipf@newsbf01.news.aol.com> In article , L.V.Bystrykh writes: The fungus you were growing was probably not a mushroom but sounds like the classic description of Kombucha. paul stamets From mb1gt at silver.shef.ac.uk Fri Oct 21 07:58:50 1994 From: mb1gt at silver.shef.ac.uk (G Turner) Date: Mon Mar 7 09:15:38 2005 Subject: EMBO workshop Filamentous Fungi References: Message-ID: <388dua$51g@hippo.shef.ac.uk> John J Weiland (jweiland@BADLANDS.NODAK.EDU) wrote: : Can anyone relay information to me about the 1995 EMBO workshop on : Filamentous Fungi (if it is in 1995)? Where will it be and when? What is : the due date for abstracts and how much is registration? : THanks! : John Weiland jweiland@badlands.nodak.edu John, The next EMBO meeting will be in Munster in 1996, as far as I recall. For further information, contact vandenHondel@MBL.TNO.NL - Cees van den Hondel, who organized the last one No information has yet been sent out on this. All the best Geoff Turner G.Turner@sheffield.ac.uk From Sergi.Ferrer at uv.es Fri Oct 21 07:29:01 1994 From: Sergi.Ferrer at uv.es (Sergi Ferrer) Date: Mon Mar 7 09:15:38 2005 Subject: Myconet References: <36863.142953160@techlink.techlink.fr> Message-ID: In article <36863.142953160@techlink.techlink.fr> lionel@techlink.fr (Lionel Benhamou) writes: >I tried two times to join Myconet without success. >Am I the only one ? >A bientot. >lionel@techlink.fr Sure not! Time ago it's not working. I don't know why. Sergi Ferrer Departament de Microbiologia Phone: +34 (6) 3864390 Facultat de Biologia Fax: +34 (6) 3864372 Universitat de Valencia E-mail: Sergi.Ferrer@uv.es E-46100 Burjassot-Valencia, SPAIN From biohelp Fri Oct 21 04:00:13 1994 From: biohelp (BIOSCI Administrator) Date: Mon Mar 7 09:15:39 2005 Subject: UNSUBSCRIBING, BIOSCI ARCHIVES, ADDRESS DATABASE & BIOSCI FAQ Message-ID: <199410210900.CAA28192@net.bio.net> Four important items follow: How to cancel e-mail subscriptions to BIOSCI newsgroups, BIOSCI archive searching, the BIOSCI FAQ, and the BIOSCI User Address Directory form. If you have not yet listed yourself in our BIOSCI user directory, please take a few minutes to complete and return the form below. If your personal information has changed since you listed yourself, please send us a complete new updated form. We can not make manual revisions to existing entries. 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Thanks again for your cooperation! --------------- please cut here and return portion below --------------- New information or Update to old record (enter N or U): date (DD-MM-YY): first name: middle initial: family name: job title: e-mail address: e-mail network: phone number: FAX number: institution: address1: address2: address3: city: state/province: country: postal code: research interest: research interest: comment: comment: comment: comment: comment: From S.Yussof at unsw.EDU.AU Fri Oct 21 02:02:43 1994 From: S.Yussof at unsw.EDU.AU (Shaharin Yussof) Date: Mon Mar 7 09:15:39 2005 Subject: ATCC & Psilocybe References: <9410072239.AA18587@umailsrv1.UMD.EDU> Message-ID: <1994Oct21.070243.29923@usage.csd.unsw.OZ.AU> In article <9410072239.AA18587@umailsrv1.UMD.EDU>, Jerome_J_MOTTA@UMAIL.UMD.EDU (jm102) says: > Just to set the record straight regarding Psilocybe and ATCC, the fact is >that no one ordered, demanded or otherwise pressured ATCC into de-accessioning >Psilocybe from its inventory. ^^^^^^^^^^^^ Isn't that one of the most wonderful bit of "verbification" you have ever seen? What's wrong with the word "removing"? Shah From sannis at uoguelph.ca Thu Oct 20 16:24:13 1994 From: sannis at uoguelph.ca (Seanna Annis) Date: Mon Mar 7 09:15:39 2005 Subject: PCR from fungal colonies References: <370qn3$f8v@hippo.shef.ac.uk> <374uet$5i3@nermal.cs.uoguelph.ca> Message-ID: <386n5t$91j@nermal.cs.uoguelph.ca> : J Kennedy (mb1jxk@silver.shef.ac.uk) wrote: : : I am going to be doing some PCR on fungal colonies from : : plates. As I will be screening a lot of colonies : : (from a cross of Aspergillus nidulans) I am looking for : : a quick and reliable method. Any suggestions would be : : welcome. : : j.kennedy@sheffield.ac.uk : : : In our lab we have been using a boiling prep method, very little time : involved. I believe one of the methods can be found in Biotechniques. I : have not used the method myself, but many people in our lab have, and it : usually worked well. Good luck. Seanna sannis@uoguelph.ca Sorry I have gotten my journals mixed up. The reference for this method is in Hensen et al. 1993 Phytopathology vol 83. p 283 to 287. We have used this method for extracting DNA from infected roots and leaves and from mycelium. Seanna Annis From rarnold at teleport.com Thu Oct 20 21:42:24 1994 From: rarnold at teleport.com (rarnold@teleport.com) Date: Mon Mar 7 09:15:39 2005 Subject: Boletus mirabilis Message-ID: <84528.rarnold@teleport.com> Mushrooms Demystified describes Boletus mirabis (Admirable Bolete) ~~~~~~~~~~~~~~~~~~~~~ as occuring "on or near rotting conifers (especially hemlock). I take this to suggest that this species ought to be able to be raised on wood chips. Please contact me if you have cultivated this species and/or have some spores of it you might be able to supply to me. Thanks in advance, Ralph D. Arnold rarnold@teleport.com P.O. Box 889, Oregon City, OR 97045 maintainer of "ON-LINE ACCESS" gopherspace -encouraging internet access gopher://gopher.teleport.com/11/users/rarnold From mdw3 at delphi.com Thu Oct 20 21:58:00 1994 From: mdw3 at delphi.com (mdw3@delphi.com) Date: Mon Mar 7 09:15:39 2005 Subject: More tea fungi Message-ID: Recently I received the unusual gift of a large(9"in diameter) amoeboid plasmodial "fungus" proported to be of Eygptian origin. It appearently reproduces asexually in cycles of 7-8 days and thrives immersed in amedium of cold tea and sugar. I am not a myco logist but I assumed this to be some form of myxomycetes or slime mold as per its size and consistency. but if not then. What? I would most grateful for a response thank you. Michael Wright. From jweiland at BADLANDS.NODAK.EDU Fri Oct 21 17:16:29 1994 From: jweiland at BADLANDS.NODAK.EDU (John J Weiland) Date: Mon Mar 7 09:15:39 2005 Subject: thanks re: EMBO/manipulations fungi Message-ID: Thanks to all respondants regarding the EMBO workshop and culture/mating methods for filamentous fungi. --a new disciple of the Fungal Genetics Newsletter John WEiland From nathan at cse.ucsc.edu Fri Oct 21 14:30:42 1994 From: nathan at cse.ucsc.edu (Nathan J. Wilson) Date: Mon Mar 7 09:15:39 2005 Subject: Myconet References: <36863.142953160@techlink.techlink.fr> Message-ID: <3894t2$edk@darkstar.UCSC.EDU> Sergi Ferrer (Sergi.Ferrer@uv.es) wrote: : In article <36863.142953160@techlink.techlink.fr> lionel@techlink.fr (Lionel Benhamou) writes: : >I tried two times to join Myconet without success. : >Am I the only one ? : Sure not! Time ago it's not working. I don't know why. I know Blaise (one of the founders of Myconet) personally and haven't been able to reach him over the phone for several months. The message on his answering machine has changed so I think he's still alive, but I think Myconet is for all intensive purposes dead or at least waiting for the rain to fall in California :-). -Nathan From mdw3 at delphi.com Sat Oct 22 06:34:54 1994 From: mdw3 at delphi.com (mdw3@delphi.com) Date: Mon Mar 7 09:15:39 2005 Subject: Kombucha Message-ID: None Myc, I'm seeking to know if this is the same yeast/bacterium that has recently become "faddish" in LA etc and if so, why? ie what sort of properties and claims are being ascribed to it credible and incredible. Michael Wright. From REDHEADS at NCCCOT.AGR.CA Sat Oct 22 12:54:46 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:39 2005 Subject: Tea Mushroom aka Kambucha Message-ID: <01HIKV7AL6TE003L2Z@GW.AGR.CA> Regarding the Tea-Mushroom which equals Kambucha it is a yeast bacteria mixture. There is a nice article on it in The Mycologist 7(1): 12-13. 1993, by Kappel & Anken. The organisms are: Bacterium xylinum, B. xylinoides. B. gluconium, and B. ketogenum, and the yeasts Saccharomyces ludwigii, Schizosaccharomyces pombe and Pichia fermentans. DRINK IN GOOD HEALTH I GUESS! Scott From todell at u.washington.edu Sat Oct 22 12:30:56 1994 From: todell at u.washington.edu (Thomas O'Dell) Date: Mon Mar 7 09:15:39 2005 Subject: Boletus mirabilis In-Reply-To: <386alp$16j7@bigblue.oit.unc.edu> References: <386alp$16j7@bigblue.oit.unc.edu> Message-ID: not all fungi that fruit on wood are saprobes (and therefore potentially cultivated. B. mirabilis is one example of an ectomycorrhizal fungus fruiting on well-decomposed wood, but presumably obtaining most of its energy from a living host tree. there are however a couple of references to boletes fruiting in pure culture (e.g. Pantidou, M.E. 1964. Cultural studies of Boletaceae: Carpophores of Xerocomus badius and Xerocomus illudens in culture. Can. J. Bot. 42:1147-1149.). to the best of my knowledge no one has ever been able to repeat these results... hope this is informative. From epichloe at KAOS.AUM.EDU Sat Oct 22 13:43:52 1994 From: epichloe at KAOS.AUM.EDU (James White) Date: Mon Mar 7 09:15:39 2005 Subject: request for contacts Message-ID: I am in need of some assistance in identifying one or more mycologists located in the area of Mexico City, Mexico. I am trying to arrange a collecting trip to obtain a member of the Clavicipitaceae that occurs in the mountains in that part of Mexico. Does anyone know of a mycologist in that area that may be willing to assist a little? Perhaps someone at a mycological herbarium in one of the local universities. I will be very appreciative for any suggestions. Jim White Dept. of Biology Auburn University at Montgomery Montgomery, AL 36117 From aday at julian.uwo.ca Sat Oct 22 16:33:44 1994 From: aday at julian.uwo.ca (a.w. day) Date: Mon Mar 7 09:15:39 2005 Subject: NEW - Fungal Genetics Teaching Software Message-ID: <38c0fo$d82@falcon.ccs.uwo.ca> Announcement - New Fungal Genetics teaching software The Virtual Genetics Laboratory is an integrated series of highly interactive experiments - follow a set of fungal mutants from isolation to characterization to tests for allelism to mapping via random spore, tetrad analysis and mitotic recombination tech niques. Perform the procedures on screen just as you would in the lab - isolate and drag samples from mutant colonies to test genotype, mix haploids in pairs to do complementation tests, score colonies growing on replica plates etc, Each exercise is preceded by an introduction clearly s etting out the purpose and goals of the experiment. Help animations and brief theory summaries are provided wherever necessary. Exercise 1 Isolation and identification of 3 auxotrophic mutants. You are presented with a pair of replica plates following mutagen treatment. Pick off cells from a mutant colony, inoculate them on minimal medium, then test 8 potential growth factors to identify the requirement. Repeat with remaining two mutants. Exercise 2. Analysis of mutants with similar phenotypes - Biochemical test to see which are allelic. You are given 10 arginine-requiring mutants. Inoculate each to minimal medium, then test 4 precursors of arginine to see which support growth. Deduce which of the genes in the known arginine pathway are mutant in each of the 10 strains. Exercise 3. Analysis of mutants with similar phenotypes - Genetical test to see which are allelic (Complementation Test). You are given 10 histidine-requiring mutants. Mate these in all possible combinations to make diploids and record the phenotype of the diploid that is formed. Deduce from this complementation data how many histidine- requiring genes are present and which mutants are allelic. Help files feature an animated-lesson covering the theory of complementation Exercise 4. Mapping by random-spore techniques - 3-point cross You are presented with a sample of 16 individual segregants from a 3-point cross that have been replicated to 5 test media. Deduce the genotype of each in turn, then using the much larger sample of segregants provided calculate the % recombination for eac h gene pair, make a map and answer questions about map distances, interference etc. 2 Help animations on the principles and practice of mapping included Exercise 5. Mapping using Tetrad Analysis - the same 3 mutants as in #4 plus a new orange colony colour marker. You are presented with a sample of 8 asci from a 4-point cross. i) Gene to centromere mapping - first score each ascus for First division and Second division segregation - then using data from a larger sample calculate the gene-to centromere distance for each of the 4 markers. ii) Gene to gene mapping - now using the same sample of 8 asci score them for PD, NPD or T-type tetrads and from the larger sample estimate gene-to gene recombination for each of the 6 possible gene combinations. Using the combined data from exercises 4 a nd 5 build the best possible map for the 4 genes and their centromeres. Help animation on Tetrad analysis included. Exercise 6. Mapping via Mitotic Recombination - exploiting haploidization and mitotic crossing- over to obtain further mapping data. You are given 9 segregants from a diploid fungus and asked to score them for i) ploidy ii) different auxotrophic and drug resistance markers. The segregants are then analysed for mapping information. A full length 'help' animation on mitotic recombination is included. This topic makes students think about what happens during mitosis and what wo uld happen if a rare recombinational event occurred. Program runs on PC computers running Windows 3.x and is also available integrated into our very comprehensive animated Genetics text - Visual Genetics - now used by over 100 institutions world-wide. Program now available at modest cost. For details contact:- aday@julian.uwo.ca From rarnold at teleport.com Sat Oct 22 22:30:34 1994 From: rarnold at teleport.com (Ralph D. Arnold) Date: Mon Mar 7 09:15:39 2005 Subject: request for contacts Message-ID: <87177.rarnold@teleport.com> On 22 Oct 1994 11:43:52 -0700, James White wrote: >I am in need of some assistance in identifying one or more mycologists >located in the area of Mexico City, Mexico. I am trying to arrange a >collecting trip to obtain a member of the Clavicipitaceae that occurs in >the mountains in that part of Mexico. > >Does anyone know of a mycologist in that area that may be willing to assist >a little? Perhaps someone at a mycological herbarium in one of the local >universities. I will be very appreciative for any suggestions. > >Jim White >Dept. of Biology >Auburn University at Montgomery >Montgomery, AL 36117 > > MYCOLOGISTS ONLINE lists one person in Mexico with an email address. Perhaps she will know someone in the area you want. Below is a copy of that entry: //////////////////////////////////////////////////////////////////////// LOPEZ-FRANCO, Rosamaria Centro Desarrollo Biotech., Inst. Technologicy y de Estudios Superiores de Monterrey -Sucrusal de Correos J. Monterrey, N. L., 64849, Mexico. ///////////////////////////////////////////////////////////////////////// Ralph D. Arnold rarnold@teleport.com P.O. Box 889, Oregon City, OR 97045 maintainer of "ON-LINE ACCESS" gopherspace -encouraging internet access gopher://gopher.teleport.com/11/users/rarnold From staben at seqanal.mi.uky.edu Sun Oct 23 11:10:41 1994 From: staben at seqanal.mi.uky.edu (Chuck Staben) Date: Mon Mar 7 09:15:39 2005 Subject: Neurospora genome project Message-ID: <38e1u1$ofl@s.ms.uky.edu> Jay Dunlap reminded me that he had taken some responsibility for organizing an effort to use CHEF gels of translocation strains to generate chromosome arms for probing the YAC libray generated by Mike Centola. There has not been anyone strongly motivated enough to really do this. Jack Kinsey and Jay Dunlap have some limited funds available for this project. David Catcheside informed me that his group has done two chromosome walks: one on LGV covering 240 kb covering gul and ure-1. A second covers cog and probably contains ad-3A and ad-3B. Many other groups, such as Jay Dunlap and Tom Schmidhauser and Rob McClung have also reported chromosome walks, though it is my impression that these data are not really being accumulated in any central fashion. Dan Ebbole has recently acquired the YAC library from FGSC, and suggests that FGSC might provide hybridization filters as a service. My impression is that there is not a well-coordinated effort to map the Neurospora genome at present, and that this remains a fertile area for discussion at Asilomar. Chuck From Lenja Mon Oct 24 03:28:08 1994 From: Lenja (Lenja) Date: Mon Mar 7 09:15:39 2005 Subject: Strange fungi in burned forest Message-ID: Hi I did not work with fungi too much although I am microbiologist. During my student project I collected some strange fungi in an Ural Region in Russia. There was a fire in forest about week before I've started. An amasing fact was that a lot of colored plasmodia and cup-like fungi (like a small coffee cup) were growing in plenty on a groung where all other plants and grass were burned out. I could never find the same species in a neighbourhood where fire din not happened. Apparently a forest fire is a disaster, but seems to be a great fun for mycologist. Has anybody in USA studied fungi in burned forests? About medicinal tea. The fungus I saw in use for the tea was not plasmodium, neither colored. It formed a sheet layer with some gelly stuff beneath. Lenja From lorelei at U.WASHINGTON.EDU Mon Oct 24 00:44:27 1994 From: lorelei at U.WASHINGTON.EDU (Lorelei Norvell) Date: Mon Mar 7 09:15:39 2005 Subject: Help finding a gelatinous mushroom ... References: <38fdli$f6t@newsbf01.news.aol.com> Message-ID: On 24 Oct 1994, William783 wrote: > But I've was told about a gelatinous mushroom that grows is Oregon. > I actually saw one in Oct of '92 (my how times flies) at a Mychology > workshop. I didn't write the name down. Can anyone guide me....> William. We shall probably need a little more information than you have given us. If the mushroom was the consistency of jelly and an edible, you may be referring to Auricularia auricula (sometimes called a Tree Ear) which is brown colored. There are two types of Witche's Butter (Tremella mesenterica and Dacrymyces palmatus) which are brilliant yellow. There is a little whitish mushroom with "teeth" on the underside sometimes called the Jelly Tooth (Pseudohydnum gelatinosum). And there is also the orange colored Phlogiotis helvelloides(Apricot Jelly Mushroom). Other possibilities include Exidia glandulosa, Tremella cerebriformis, Guepiniopsis (Heterotextus) alpina, and Calocera viscosa, among others. Could you describe what you remember? Lorelei From william783 at aol.com Sun Oct 23 23:37:06 1994 From: william783 at aol.com (William783) Date: Mon Mar 7 09:15:39 2005 Subject: Help finding a gelatinous mushroom ... Message-ID: <38fdli$f6t@newsbf01.news.aol.com> It is probably all too obvious that I'm not a mycologist. But I've was told about a gelatinous mushroom that grows is Oregon. I actually saw one in Oct of '92 (my how times flies) at a Mychology workshop. I didn't write the name down. Can anyone guide me to what it is called and if there are more than one. I'm particularly interested in its range, and in any native american uses of it. A woman in California who makes paper out of mushrooms said at this workshop that it could be use as a sizing. Any help will be appreciated. And good hunting this Autumn. William From todell at u.washington.edu Mon Oct 24 11:11:31 1994 From: todell at u.washington.edu (Thomas O'Dell) Date: Mon Mar 7 09:15:39 2005 Subject: symbiosis In-Reply-To: <94102409463026@hugo.fsufay.edu> References: <94102409463026@hugo.fsufay.edu> Message-ID: legumes (especially sub genera Papillionoideae and Mimosoideaea) tend to be highly va mycorrhiza dependant. the plant-fungal-bacterial symbiosis is commly referred to as tripartite and has been the subject of considerable research. certainly hundreds of papers exist on the topic particularly re soybean, clover and other ag. crop plants. actinorrhizal (frankia-nodulated) plants also tend to be mycorrhizal, va or ecto (e.g. alnus; dryas) depending on the plant species. From Mullin at nbnet.nb.ca Mon Oct 24 10:28:34 1994 From: Mullin at nbnet.nb.ca (Robert Mullin) Date: Mon Mar 7 09:15:39 2005 Subject: Info. about T. ponderosum and T. caligatum Message-ID: <38gjr2$9s8@darwin.nbnet.nb.ca> I am ultimately attempting to grow, on a commercial level, either the T. ponderosum or the T. caligatum and I am starting from scratch. I am not that familiar about how to grow these species and I would appreciate any help in locating relevant information. Thanks in advance Robert Mullin From dhaas at HUGO.FSUFAY.EDU Mon Oct 24 08:52:09 1994 From: dhaas at HUGO.FSUFAY.EDU (dhaas@HUGO.FSUFAY.EDU) Date: Mon Mar 7 09:15:39 2005 Subject: symbiosis Message-ID: <94102409463026@hugo.fsufay.edu> I had a student ask an interesting question this AM. "Are there any plants that form mutualistic relationships with both bacteria and fungi at the same time?" I told her that I couldn't give her a specific example but I didn't see why it couldn't happen. Does anyone know of examples of this? Do legumes containing Rhizobium also show endo or ecto mycorrhizal associations? Dave From mlsbb3embm at dct.ac.uk Mon Oct 24 07:47:52 1994 From: mlsbb3embm at dct.ac.uk (mlsbb3embm@dct.ac.uk) Date: Mon Mar 7 09:15:39 2005 Subject: Help...PLEASE!!! Message-ID: <1994Oct24.124752.1@dct.ac.uk> I hope someone here can help me with a fictitios grant proposal I have to make. The topic I've been given is to develop a research programme around an expedition to the Amazonian Rainforest to isolate new wood decay fungi for potential use in the pulp and papermaking industries, also including costing of the proposals. As it is I don't have a clue, so I would appreciate if you could post (or e-mail) information regardin the following: wood decay fungi; the pulp and papermaking industries; grant proposals; and costing. Many thanks, Ewan Mollison. From jfrigot at ucdavis.edu Mon Oct 24 14:00:23 1994 From: jfrigot at ucdavis.edu (Jerome F Rigot) Date: Mon Mar 7 09:15:39 2005 Subject: fungus tea References: Message-ID: <38h087$obv@mark.ucdavis.edu> Hello, I am perplexed by your inquiry about Kombucha tea. I thought that Kombucha was a japanese tea, made with kombu, a seaweed. It is a tea commonly drunken in Japan, and I never heard anything about it being related to mushroom tea. So I supposed that you may be reffering to something different. Can you tell me about it? Jerome F Rigot Department of Environmental Toxicology University of California Davis, CA 95616 (916)752-5896 e-mail: jfrigot@envtox.ucdavis.edu or: jfrigot@ucdavis.edu From CABI-BIONET at CABI.org Mon Oct 24 12:42:00 1994 From: CABI-BIONET at CABI.org ("BIONET International ", CABI) Date: Mon Mar 7 09:15:39 2005 Subject: (none) Message-ID: <2EABF1D9@smtp-gateway.cgnet.com> To all concerned with the systematics of invertebrates and microorganisms BioNET-INTERNATIONAL is a Global Technical Cooperation Network of institutions and people concerned with biosystematics of Invertebrates and Microorganisms. The goal of this network is the mobilisation and enhancement the world's biosystematic resources for the benefit of developing countries. BioNET-INTERNATIONAL is building and sustaining realistic biosystematic self-reliance in developing sub-regions and providing the biosystematic backup to biodiversity. BioNET-INTERNATIONAL is convening a Global Workshop (BIGW, 1995) in Cardiff, Wales on 23-26 August 1995 to provide an interface between the providers and users of biosystematic information and the donors who fund biosystematic projects. The BIGW will: * Alert governments, international organisations and donors to the present, critical, biosystematic impediment to sustainable agricultural development and the wise-use of biodiversity in developing countries, and will recommend action to solve the problem. * Review BioNET-INTERNATIONAL's current role and achievements, its objectives, scope and progress, and devise operational and financial strategies for its further development. * Review and inventory the needs of developing country LOOPs and the resources on offer to them through developed country LOOPs (BIOCON), and devise costed work programmes and structures for donor-funding to deliver resources and satisfy needs. For more information on BioNET-INTERNATIONAL or on BIGW, 1995 please write to us at the address above or send an E.Mail message with your postal address to CABI- BioNET@CABI.ORG. Yours sincerely Dr Mark Cook on behalf of Professor Tecwyn Jones, Director BioNET-INTERNATIONAL Technical Secretariat. _____________________________________________ E.Mail Message from the BioNET-INTERNATIONAL Technical Secretariat 56 Queen's Gate, London, SW7 5JR, U.K. Tel: +44-171-584 0067. Fax: +44-171-581-0067. E.Mail: CABI-BioNET@CABI.ORG Reference: MAC/BI/284 ___________________________________________________________ From REDHEADS at NCCCOT.AGR.CA Mon Oct 24 19:51:54 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:39 2005 Subject: Burn site fungi Message-ID: <01HIO2D96UGI0041HN@GW.AGR.CA> Regarding burn site fungi- in Canada you might try contacting Keith Egger at "kegger@kean.ucs.mun.ca" He studies cup fungi associated with burns. From EVERSL at AXE.HUMBOLDT.EDU Mon Oct 24 23:55:05 1994 From: EVERSL at AXE.HUMBOLDT.EDU (EVERSL@AXE.HUMBOLDT.EDU) Date: Mon Mar 7 09:15:39 2005 Subject: Mexico contacts Message-ID: <01HIO4ER1YS08WWGED@AXE.HUMBOLDT.EDU> In 1992, the FOWM (Foray of Western Mycolgists) met in Northern California. Dr. J. Trappe (Oregon State University) was here with two mycologists from Mexico. Perhaps he can help you. Linda Evers eversl@axe.humboldt.edu From sannis at uoguelph.ca Mon Oct 24 16:04:03 1994 From: sannis at uoguelph.ca (Seanna Annis) Date: Mon Mar 7 09:15:40 2005 Subject: Help...PLEASE!!! References: <1994Oct24.124752.1@dct.ac.uk> Message-ID: <38h7g3$3f3@nermal.cs.uoguelph.ca> mlsbb3embm@dct.ac.uk wrote: : I hope someone here can help me with a fictitios grant proposal I have : to make. : The topic I've been given is to develop a research programme around an : expedition to the Amazonian Rainforest to isolate new wood decay fungi for : potential use in the pulp and papermaking industries, also including costing of : the proposals. : As it is I don't have a clue, so I would appreciate if you could post : (or e-mail) information regardin the following: wood decay fungi; the pulp and : papermaking industries; grant proposals; and costing. : Many thanks, : Ewan Mollison. I would suggest going to the library and looking up industrial mycology. There is a lot of interest in using fungal enzymes ie from wood decay fungi to break down lignin etc in wood, avoiding the use of chlorine and other chemicals to bleach the pulp. You probably will also want to look up wood decay fungi, or basidiomycetes as a broad group, and look for habitat range, if possible they could be found in the rainforest. Supposedly you may find new species so if in your proposal you can state that other fungi are found in the area there is a good chance you may find some new species. Typically funding is vague, you should ask your prof. how exact they want it. Think of travel costs, labour costs, living expense, and rough lab equipment costs, people typically estimate these on grants anyways. As for writing a grant proposal, it has to be concise reasonably detailed. See if your prof has an example. Good luck, this is an interesting area of research. Seanna (sannis@uoguelph.ca) From thanh at wwg3.uovs.ac.za Tue Oct 25 06:18:49 1994 From: thanh at wwg3.uovs.ac.za (thanh@wwg3.uovs.ac.za) Date: Mon Mar 7 09:15:40 2005 Subject: ** What are yeast? ** Message-ID: Hi! For most of people yeasts are Sac. cerevisiae. For most of yeastists yeasts are the fungi with single cells stage of development. But Mucor spp. can give us such kind of cells in condition without O2 as well. So, how do you think about "what are yeast" and "their origin"? origin of yeasts: / ... / fungi1 / yeasts Fungi - ... or yeast - fungi2 or X - fungi \ yeasts \ fungi... \ ... or ... Have a nice day! Thanh -------------------------------------------- Vu Nguyen Thanh Dept. Microbiology & Biochemistry The University of the Orange Free State PO Box 339, Bloemfontein 9300, South Africa -------------------------------------------- From rpeter at nmsu.edu Tue Oct 25 02:10:10 1994 From: rpeter at nmsu.edu (Peter Herman x5495) Date: Mon Mar 7 09:15:40 2005 Subject: symbiosis References: <94102409463026@hugo.fsufay.edu> Message-ID: <38ib0i$g9r@dns1.NMSU.Edu> In article <94102409463026@hugo.fsufay.edu> dhaas@HUGO.FSUFAY.EDU writes: >I had a student ask an interesting question this AM. "Are there any plants >that form mutualistic relationships with both bacteria and fungi at the same >time?" I told her that I couldn't give her a specific example but I didn't >see why it couldn't happen. Does anyone know of examples of this? Do legumes >containing Rhizobium also show endo or ecto mycorrhizal associations? > >Dave You are correct in your assumption that legumes can be nodulated by rhizobia and be mycorrhizal as well. I know for sure it occurs in Pisum, Trifolium and Medacago having read papers on all these with nodules and VAM. I don't know for sure if Alnus or Myrica and the other streptomycete symbionts but I would be surprised if it is not the case Peter Herman Biology New Mexico State Univ. From Tom Tue Oct 25 10:02:44 1994 From: Tom (Tom) Date: Mon Mar 7 09:15:40 2005 Subject: ideas for slime mold projects? Message-ID: <38j6mk$587@mustang.alleg.edu> One of my Intro. Biology students last year took an interest in slime molds and now wants do some independent research with them. I'd like to encourage her but my background is in plant physiology and development and I know almost nothing about slime molds. Can anyone in this group suggest ideas for undergraduate-level slime mold projects? Or alternately, are there any good books or other references we could go to for ideas? So far we've had an easy time of growing up Physarum from spores and watching it (them?) move around on an agar plate, but that's getting boring fast. Thanks in advance for any and all help. Tom Goliber (tgoliber@alleg.edu) Dept. of Biology Allegheny College From rarnold at teleport.com Tue Oct 25 09:02:50 1994 From: rarnold at teleport.com (Ralph D. Arnold) Date: Mon Mar 7 09:15:40 2005 Subject: Clitocybe nuda? Message-ID: <38j36a$33r@mserv1.dl.ac.uk> Would you please help me? I am seeking info on the cultivation of Clitocybe nuda (=Lepista nuda). I was informed that this species is being raised in the UK. Would you be kind enough to "point" me towards sources of information concerning the cultivation - perhaps even commercially - of this fine fungi? Let me thank you in advance for any assistance, Ralph D. Arnold Ralph D. Arnold rarnold@teleport.com P.O. Box 889, Oregon City, OR 97045 maintainer of "ON-LINE ACCESS" gopherspace -encouraging internet access gopher://gopher.teleport.com/11/users/rarnold From stamets1 at aol.com Tue Oct 25 13:42:04 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:40 2005 Subject: are there any poisonous polypores ? Message-ID: <38jjhs$knu@newsbf01.news.aol.com> Besides those ingesting MAO inhibitors and some reports of polypores growing on Eucalyptus being occasionally toxic, does anyone know of a truly poisonous polypore? Please include any references, if possible. paul stamets po box 7634 olympia, wa. 98507 From mflorian at SUN10.INF.UNITN.IT Wed Oct 26 08:28:16 1994 From: mflorian at SUN10.INF.UNITN.IT (Marco Floriani) Date: Mon Mar 7 09:15:40 2005 Subject: fungi on burnt ground Message-ID: <9410261326.AA01169@sun10.inf.unitn.it> > Hi > I did not work with fungi too much although I am microbiologist. > During my student project I collected some strange fungi in an Ural > Region in Russia. There was a fire in forest about week before I've started. > An amasing fact was that a lot of colored plasmodia and cup-like fungi (like a > small coffee cup) were growing in plenty on a groung where all other plants and > grass were burned out. I could never find the same species in a neighbourhood where fire > din not happened. > Apparently a forest fire is a disaster, but seems to be > a great fun for mycologist. Has anybody in USA studied fungi in burned forests? > > About medicinal tea. The fungus I saw in use for the tea was not plasmodium, > neither colored. It formed a sheet layer with some gelly stuff beneath. > > Lenja Rovereto, October 26, 1994. Hi Lenja ! I'm Marco Floriani, an Italian computer student very fond of mycology. I've read your message about the fungi you have found and I'm very interested about the cup-like ones (not the plasmodiums). If you want to give me some information about them you can write me. I like studying Ascomycetes (especially the ones of a certain size) and I hope someone else will write about the interesting species they find (I would like to know especially something about the genus Peziza). If someone knows the Internet address of some other "Ascomycetologist" (may I use this word?) please let me know. Marco Floriani Via Vigolana, 8 I-38057 Pergine Valsugana (TN) Internet address: mflorian@sun10.info.unitn.it From CABI-BIONET at CABI.org Wed Oct 26 08:13:38 1994 From: CABI-BIONET at CABI.org ("BIONET International ", CABI) Date: Mon Mar 7 09:15:40 2005 Subject: (none) Message-ID: <2EAE55F8@smtp-gateway.cgnet.com> To European biologists concerned with the systematics of invertebratesb and microorganisms ____________________________ First Announcement of EuroLOOP Workshop II Amsterdam, 27-30th March 1995 ____________________________ EW II is the sequel to the First Workshop held in Tervuren, Belgium in June 1994 where representatives from institutions in 22 European countries and from three International Institutes founded EuroLOOP as a part of BioNET-INTERNATIONAL - the global network for biosystematics of Invertebrates and Microorganisms. EuroLOOP seeks to mobilise, pool and enhance the biosystematic resources of its member centres of excellence, and through donor-funded programmes make these available to BioNET- INTERNATIONAL LOOPs in developing countries to support national programmes for sustainable agriculture and use of biodiversity. EuroLOOP aims to provide the EU, and the national and federal governments of Europe and developing countries, with the best possible biosystematic advice and services within its remit, to assist unilateral and multilateral aid programmes for conservation and wise use of the environment. As a powerful lobby within the EU and governances of Europe, EuroLOOP seeks to ensure requisite investment in the biosystematic institutions of the continent to enable the generation and delivery of vital new knowledge and better technologies needed by agricultural, environmental and biodiversity practioners world-wide. EW II will set the agenda for the further development of EuroLOOP and formulate strategies for achieving its objectives. It will provide global and regional perspectives of resources, deficiencies and needs and through collaborative work-programmes and recommendations, undertake and recommend effective actions through EuroLOOP for removing the present biosystematic impediment to biological research and development. For further information please contact: Dr. Eliane de Coninck EuroLOOP Network Coordinating Institute Koninklijk Museum Voor Midden-Afrika Leuvensesteenweg 13 B-3080 Tervuren BELGIUM. Tel: +32-2-769 5386/5360/5373 Fax:+32-2-767 0242 ________________________________________________________ This message was posted by the BioNET-INTERNATIONAL Technical Secretariat on behalf of EuroLOOP. We will forward E.Mail messages to CABI-BioNET@CABI.ORG to the EuroLOOP Secretariat. _________________________________________________________ From biochem at clio.rz.uni-duesseldorf.de Wed Oct 26 11:25:46 1994 From: biochem at clio.rz.uni-duesseldorf.de (Weiss) Date: Mon Mar 7 09:15:40 2005 Subject: ** What are yeast? ** Message-ID: <38lvua$l7o@mserv1.dl.ac.uk> In his mail "** What are yeast? **" Vu Nguyen Thanh wrote: >Hi! >For most of people yeasts are Sac. cerevisiae. >For most of yeastists yeasts are the fungi with single cells stage of >development. But Mucor spp. can give us such kind of cells in condition >without O2 as well. >So, how do you think about "what are yeast" and "their origin"? > origin of yeasts: > / ... / fungi1 / yeasts > Fungi - ... or yeast - fungi2 or X - fungi > \ yeasts \ fungi... \ ... > or ... >Have a nice day! >Thanh I've thought it over a while and came to this conclusion. Since there are some Zygomycete and Basidiomycete yeasts beneath the common Ascomycetes, my proposal is: ... /Neurospora / /more fungi /lots of fungi Fungi -even more fungi -mushrooms (mnjam) -... \yeasts \some more poor yeasts - \ To say it short, yeasts are just degenerated filamentous fungi! (Sorry to al "yeastists"!) Warning: This sketch isn't based on any rRNA sequences. It is in fact the somewhat eccentric view of an molecular biology PhD candidate working with a filamentous fungus and not with yeast. Christoph Kruell, Universitaet Duesseldorf, Germany From A.F.Brown at bradford.ac.uk Wed Oct 26 14:46:36 1994 From: A.F.Brown at bradford.ac.uk (FRED) Date: Mon Mar 7 09:15:40 2005 Subject: (serious) prehistoric use of fungi Message-ID: I'm trying to find information on how our ancestors perhaps used fungi in their lives. I've come across a few cases, such as the man in the ice near Austria found recently, but I want more than just one photo and a paragraph. The medicinal side of things is an interest of mine, but I would love to hear of any leads investigating the ritual use of psychoactive fungi within these cultures. I'm studying Archaeology at Bradford, and I would like some serious feedback and not just opinions on 'cool trips' and the like. Ta. From lmelvill at uoguelph.ca Wed Oct 26 12:56:06 1994 From: lmelvill at uoguelph.ca (Lewis Melville) Date: Mon Mar 7 09:15:40 2005 Subject: symbiosis References: <94102409463026@hugo.fsufay.edu> <38ib0i$g9r@dns1.NMSU.Edu> Message-ID: <38m57m$hnl@nermal.cs.uoguelph.ca> Peter Herman x5495 (rpeter@nmsu.edu) wrote: : >I had a student ask an interesting question this AM. "Are there any plants : >that form mutualistic relationships with both bacteria and fungi at the same : >time?" Alnus sp. ( alder ) forms an association with Frankia ( an actinomycete ) and Alpova sp. ectomycorrhizal fungi. Dryas sp. forms an association with Frankia and many types of ectomycorrhizal fungi, such as Hebeloma sp., Cenococcum sp., Laccaria sp.. Both Alnus and Dryas occur in northern and Boreal forest, often in nutrient depleted zones or recently glaciated regions. It appears as though they need the extra help to establish themselves in difficult growing areas. The Frankia provides a Nitrogen boost, and the fungi colonize the roots for sugars produced by the plant and fungal hyphae grow out into the soil and acquire Phosphorus and other minerals which are not accessible to the plant. Or so the theory goes... There are also nitrogen fixing bacteria found in association with several ectomycorrhizal fungi in the mantle which surrounds the plant roots. The root, bacteria, and fungi, also interact with other soil organisms such as nematodes, all the way across the chain to small mammals which dig up truffles of hypogeous mycorrhizal fungi, eat them, and then disperse the spores of the fungi in their excrement, etc, etc,.... Soil microenvironments are very complex........ lewis melville From lmelvill at uoguelph.ca Wed Oct 26 12:41:25 1994 From: lmelvill at uoguelph.ca (Lewis Melville) Date: Mon Mar 7 09:15:40 2005 Subject: Strange fungi in burned forest References: Message-ID: <38m4c5$hnl@nermal.cs.uoguelph.ca> LenjaV.Bystrykh wrote: : Hi : I did not work with fungi too much although I am microbiologist. : During my student project I collected some strange fungi in an Ural : Region in Russia. There was a fire in forest about week before I've started. : An amasing fact was that a lot of colored plasmodia and cup-like fungi (like a : small coffee cup) were growing in plenty on a groung where all other plants and : grass were burned out. I could never find the same species in a neighbourhood where fire : din not happened. : Apparently a forest fire is a disaster, but seems to be : a great fun for mycologist. Has anybody in USA studied fungi in burned forests? Ectendomyorrhizal fungi are common in post-fire sites. Maybe a Wilcoxina sp.. Keith Egger is an expert on Post-fire ascomycetes. He used to be at Memorial University in St. Johns, Newfoundland, Canada, but may be back in western Canada. lew From todell at u.washington.edu Wed Oct 26 12:34:46 1994 From: todell at u.washington.edu (Thomas O'Dell) Date: Mon Mar 7 09:15:40 2005 Subject: (serious) prehistoric use of fungi In-Reply-To: References: Message-ID: The definitive treatment on ethnomycology is Mushrooms, Russia and History by R. Gordon and Valentina Wasson. published ca. 1957. unfontunately we have little information re paleo or prehistoric use of fungi... also unfortunately the cited reference is a rare book, but i'm sure they have it at Kew. good luck! From AB143 at kdw01.kj.yamagata-u.ac.jp Wed Oct 26 16:56:43 1994 From: AB143 at kdw01.kj.yamagata-u.ac.jp (Keitaro Tawaraya) Date: Mon Mar 7 09:15:40 2005 Subject: help:extraction of hyphae Message-ID: I am now measuring the hyphal growth of arbuscular mycorrhizal fungi on sand medium. Hyphae tightly adhere to sand so extraction of hyphae is unsuccessful. Dose anybody have any information about the separation of hyphae from sand (enzymatically, chemically) ? Can anybody tell me the mechanisms of adhesion ? Any help would be appreciated. -- Keitaro Tawaraya Yamagata University E-mail:AB143@kdw01.kj.yamagata-u.ac.jp Phone:0235-23-1521(2402) Fax:0235-24-1801 From prabhuv at duke.usask.ca Wed Oct 26 11:35:30 1994 From: prabhuv at duke.usask.ca (Vikram Prabhu) Date: Mon Mar 7 09:15:40 2005 Subject: symbiosis In-Reply-To: <38ib0i$g9r@dns1.NMSU.Edu> References: <94102409463026@hugo.fsufay.edu> <38ib0i$g9r@dns1.NMSU.Edu> Message-ID: Plants that have N fixing bacteria such as soybean form VA mycorrhizae, and others like Alnus form ectendo and ectomycorrhizae (perhaps VA too) and there are several good studies demonstrating that N fixation increases the phosphate demand (thru increased growth) which is supplied by the fungus (or vice versa; chicken or egg argument). From CABI-BIONET at CABI.org Thu Oct 27 04:43:18 1994 From: CABI-BIONET at CABI.org ("BIONET International ", CABI) Date: Mon Mar 7 09:15:40 2005 Subject: European biosystematists Message-ID: <2EAF7623@smtp-gateway.cgnet.com> To European biologists concerned with the systematics of invertebrates and microorganisms ____________________________ First Announcement of EuroLOOP Workshop II Amsterdam, 27-30th March 1995 ____________________________ EW II is the sequel to the First Workshop held in Tervuren, Belgium in June 1994 where representatives from institutions in 22 European countries and from three International Institutes founded EuroLOOP as a part of BioNET-INTERNATIONAL - the global network for biosystematics of Invertebrates and Microorganisms. EuroLOOP seeks to mobilise, pool and enhance the biosystematic resources of its member centres of excellence, and through donor-funded programmes make these available to BioNET- INTERNATIONAL LOOPs in developing countries to support national programmes for sustainable agriculture and use of biodiversity. EuroLOOP aims to provide the EU, and the national and federal governments of Europe and developing countries, with the best possible biosystematic advice and services within its remit, to assist unilateral and multilateral aid programmes for conservation and wise use of the environment. As a powerful lobby within the EU and governances of Europe, EuroLOOP seeks to ensure requisite investment in the biosystematic institutions of the continent to enable the generation and delivery of vital new knowledge and better technologies needed by agricultural, environmental and biodiversity practioners world-wide. EW II will set the agenda for the further development of EuroLOOP and formulate strategies for achieving its objectives. It will provide global and regional perspectives of resources, deficiencies and needs and through collaborative work-programmes and recommendations, undertake and recommend effective actions through EuroLOOP for removing the present biosystematic impediment to biological research and development. For further information please contact: Dr. Eliane de Coninck EuroLOOP Network Coordinating Institute Koninklijk Museum Voor Midden-Afrika Leuvensesteenweg 13 B-3080 Tervuren BELGIUM. Tel: +32-2-769 5386/5360/5373 Fax:+32-2-767 0242 ________________________________________________________ This message was posted by the BioNET-INTERNATIONAL Technical Secretariat on behalf of EuroLOOP. We will forward E.Mail messages to CABI-BioNET@CABI.ORG to the EuroLOOP Secretariat. _________________________________________________________ From mflorian at SUN10.INF.UNITN.IT Thu Oct 27 04:34:31 1994 From: mflorian at SUN10.INF.UNITN.IT (Marco Floriani) Date: Mon Mar 7 09:15:40 2005 Subject: poisonous polypores Message-ID: <9410270925.AA05017@sun10.inf.unitn.it> ---------- X-Sun-Data-Type: text X-Sun-Data-Description: text X-Sun-Data-Name: text X-Sun-Charset: us-ascii X-Sun-Content-Lines: 64 > Besides those ingesting MAO inhibitors and some reports of polypores >growing on Eucalyptus being occasionally toxic, does anyone know of a >truly poisonous polypore? > > Please include any references, if possible. >paul stamets >po box 7634 >olympia, wa. 98507 Rovereto (Italy), October 27, 1994. Dear Paul, some time has passed since you asked whether someone could give you any information about poisonous species of Polyporus. I'm sorry if I answer just today, but I've discovered this Micology section on the Web just some days ago. I think that with the word "Polyporus" you meant to indicate, in a broader sense, that big group of genera which grow generally on wood and have a porose hymenium, which where some years ago called Polyporus s.l.. If I'm right, you could be glad to know thatthere is a species of Albatrellus (or Scutiger, if you prefer to call it this way) which is strongly suspected to own some kind of toxicity; its name is Albatrellus subrubescens (Murril) Pouzar 1972 (synonymous of Albatrellus similis Pouzar 1966), and it is a species closely related to the very common (or at least, very common in Europe) Albatrellus ovinus (Schaeff. ex Fr.) Kotl. & Pouz. 1957, which is notoriously an edible species (in Italy it is known as the "bread mushroom"). The differences between these two species are quite relevant: A. subrubescens owns some kind of orange hues, especially on the pores, which A. ovinus lacks. I know that A. similis owns more often then A. ovinus also a violaceous coloration. Finally, and this is perhaps the most important difference between the two fungi, the spores of A. subrubescens are amyloid, while the ones of A. ovinus are not. And you can guess if they are also macroscopically, putting a drop of Melzer's reagent on the pores. It is interesting to note that A. subrubescens is a species recently discovered, even if it seems to be at least as common as A. ovinus. Unfortunately, I can't tell you if it is common in my country, as I never checked if the distinctly violaceus Albatrellus I found had amyloid spores. But I'm almost sure they were A. subrubescens. As regards the toxicity of Albatrellus subrubescens, I can't tell you much, as it is just a suspect species. In 1986 Breitenbach wrote that it was an edible species, and so do other authors. But several other books (e.g.: R. Mazza, 1994) talk about this presumed poisonous properties, even if no mortal cases have ever verified. If you want to know something more, please tell me, and I will look for precise information. I would be glad if someone wanted to talk also about "wild" fungi and their classification, and not just about their cultivation (I do not mean that these topics are not important, howewer). The news cited above were taken from the two most widely known (in Europe) books on Aphyllophorales, which are: 1) W. Julich, 1984: Die Nichtblatterpilze, Gallertpilze und Bauchpilze Kleine Kryptogamenflora Band II Teil b/1; 2) J. Breitenbach, F. Kranzlin, 1986: Pilze der Schweiz, Band 2 Nichtblatterpilze. My address is: Marco Floriani Via Vigolana, 8 I - 38057 Pergine Valsugana (TN) Internet address: mflorian@sun10.info.unitn.it ---------- X-Sun-Data-Type: default X-Sun-Data-Description: default X-Sun-Data-Name: dead.letter X-Sun-Charset: us-ascii X-Sun-Content-Lines: 70 > Besides those ingesting MAO inhibitors and some reports of polypores >growing on Eucalyptus being occasionally toxic, does anyone know of a >truly poisonous polypore? > > Please include any references, if possible. >paul stamets >po box 7634 >olympia, wa. 98507 Rovereto (Italy), October 27, 1994. Dear Paul, some time has passed since you asked whether someone could give you any information about poisonous species of Polyporus. I'm sorry if I answer just today, but I've discovered this Micology section on the Web just some days ago. I think that with the word "Polyporus" you meant to indicate, in a broader sense, that big group of genera which grow generally on wood and have a porose hymenium, which where some years ago called Polyporus s.l.. If I'm right, you could be glad to know thatthere is a species of Albatrellus (or Scutiger, if you prefer to call it this way) which is strongly suspected to own some kind of toxicity; its name is Albatrellus subrubescens (Murril) Pouzar 1972 (synonymous of Albatrellus similis Pouzar 1966), and it is a species closely related to the very common (or at least, very common in Europe) Albatrellus ovinus (Schaeff. ex Fr.) Kotl. & Pouz. 1957, which is notoriously an edible species (in Italy it is known as the "bread mushroom"). The differences between these two species are quite relevant: A. subrubescens owns some kind of orange hues, especially on the pores, which A. ovinus lacks. I know that A. similis owns more often then A. ovinus also a violaceous coloration. Finally, and this is perhaps the most important difference between the two fungi, the spores of A. subrubescens are amyloid, while the ones of A. ovinus are not. And you can guess if they are also macroscopically, putting a drop of Melzer's reagent on the pores. It is interesting to note that A. subrubescens is a species very recently discovered, even if it seems to be at least as common as A. ovinus. Unfortunately, I can't tell you if it is common in my country, as I never checked if the distinctly violaceus Albatrellus I found had amyloid spores. But I'm almost sure they were A. subrubescens. As regards the toxicity of Albatrellus subrubescens, I can't tell you much, as it is just a suspect species. In 1986 Breitenbach wrote that it was an edible species, and so do other authors. But several other books (e.g.: R. Mazza, 1994) talk about this presumed poisonous properties, even if no mortal cases have ever verified. If you want to know something more, please tell me, and I will look for precise information. I would be glad if someone wanted to talk also about "wild" fungi and their classification, and not just about their cultivation (I do not mean that these topics are not important, howewer). The news cited above were taken from the two most widely known (in Europe) books on Aphyllophorales, which are: 1) W. Julich, 1984: Die Nichtblatterpilze, Gallertpilze und Bauchpilze - Kleine Kryptogamenflora Band II Teil b/1; 2) J. Breitenbach, F. Kranzlin, 1986: Pilze der Schweiz, Band 2 Nichtblatterpilze. My address is: Marco Floriani Via Vigolana, 8 I - 38057 Pergine Valsugana (TN) Internet address: mflorian@sun10.info.unitn.it From rwinder at PFC.Forestry.CA Wed Oct 26 17:10:07 1994 From: rwinder at PFC.Forestry.CA (Richard Winder) Date: Mon Mar 7 09:15:40 2005 Subject: Help finding a gelatinous mushroom ... References: <38fdli$f6t@newsbf01.news.aol.com> Message-ID: <1994Oct26.221007.20349@spruce.pfc.forestry.ca> In article <38fdli$f6t@newsbf01.news.aol.com>, william783@aol.com (William783) writes: >It is probably all too obvious that I'm not a mycologist. > >But I've was told about a gelatinous mushroom that grows is Oregon. >I actually saw one in Oct of '92 (my how times flies) at a Mychology >workshop. I didn't write the name down. Can anyone guide me to what it >is called and if there are more than one. Could you give us a color (orange, yellow, brown, clear, etc.) to narrow this down a bit? -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From EVERSL at AXE.HUMBOLDT.EDU Thu Oct 27 15:46:31 1994 From: EVERSL at AXE.HUMBOLDT.EDU (EVERSL@AXE.HUMBOLDT.EDU) Date: Mon Mar 7 09:15:40 2005 Subject: Neamatode agar... Message-ID: <01HIRU7ASE2A8WXPW0@AXE.HUMBOLDT.EDU> Hello, everyone, My name is Linda Evers. I'm a mycology grad student (Humboldt State Univ. Arcata, CA, USA). i'm trying to isolate naematode capturing fungi, and need to know how to establish naematodes in culture, ie. what bateria is best to maintain cultures, what agar, etc. I think I have some fungal species in culture already but I need to isolate and verify them. All help is gratefully accepted. Linda Evers eversl@axe.humboldt.edu From ISING at MACOLLAMH.UCD.IE Thu Oct 27 11:31:23 1994 From: ISING at MACOLLAMH.UCD.IE (ISING@MACOLLAMH.UCD.IE) Date: Mon Mar 7 09:15:40 2005 Subject: WRF isolation Message-ID: <38okkr$rg1@mserv1.dl.ac.uk> Dear Louis, A good medium for isolating white rot fungi is given in the following paper. Field et al., Biodegradtion of Polycyclic aromatic hydrocarbons by new isolates of white rot fungi. Applied and Environmental Microbiology, (1992) 58, 2219-2226 Once you have isolated some fungi you could try the method described by Alleman et. al. A rapid method to screen fungi for resistance to toxic chemicals. Biodegradation, 4, 125-129 If you would like copies of these refs then let me know and I will send them to you. All the best, Ian Singleton, Dept Industrial Microbiology, University College, Dublin 4, Ireland. E-mail: ising@macollamh.ucd.ie From mflorian at sun10 Thu Oct 27 10:03:31 1994 From: mflorian at sun10 (Marco Floriani) Date: Mon Mar 7 09:15:41 2005 Subject: Hygrophoraceae Message-ID: <9410271500.AA05903@sunC17.unitn.it> Hi ! I'm Marco from Italy. Is there anybody who likes studying Agaricales (I mean their classification, not just their biology or cultivation). I'm particularly interested to the family of Hygrophoraceae, especially the genera Cuphophyllus and Hygrocybe. Please tell me if someone has got some specific literature about this topics. Marco Floriani mflorian@sun10.inf.unitn.it From LOUIS at agric.up.ac.za Thu Oct 27 13:13:08 1994 From: LOUIS at agric.up.ac.za (LOUIS) Date: Mon Mar 7 09:15:41 2005 Subject: WRF isolation Message-ID: I am currently doing research on the potential of WRF to biodegrade xenobiotics. The problem is that the cultures I am working on are relative low enzyme producers. I need to isolate a few high producers of enzumes like LiP and MnP. Can someone recommend a good isolation medium for WRF from rotten wood etc. Thanks in advance Greetings from the New South Africa. Louis Environmental Biotech Lab University of Pretoria e-mail:louis@agric.up.ac.za From bncraig at bud.peinet.pe.ca Thu Oct 27 21:52:46 1994 From: bncraig at bud.peinet.pe.ca (Brian N. Craig) Date: Mon Mar 7 09:15:41 2005 Subject: Rhizoctonia on Potatoes Message-ID: <38plhe$3dm@bud.peinet.pe.ca> Are you aware of any chemical treatment that can be applied to potato tubers to eliminate rhizoctonia? Thanks for your time. Brian Craig bncraig@bud.peinet.pe.ca From stametsfam at aol.com Thu Oct 27 23:46:06 1994 From: stametsfam at aol.com (Stametsfam) Date: Mon Mar 7 09:15:41 2005 Subject: Nature of Sparassis crispa Message-ID: <38pvme$gda@newsbf01.news.aol.com> I am perplexed by Sparassis crispa. Not mycorrhizal, it does seem to be dependent on complex ecological constituents. I have gotten so far as to get a flat plateau of convoluted ridges - an aborted fruitbody - on sterilized conifer sawdust. Does anyone have any insights into its ecology or suggestions for its cultivation - indoors or outdoors in natural landscapes. Does anyone know of any successes in cultivation? Thank you very much. Paul Stamets Stamets1@aol.com From JGBENNET at SCIENCE.watstar.uwaterloo.ca Thu Oct 27 14:33:42 1994 From: JGBENNET at SCIENCE.watstar.uwaterloo.ca (J Bennett) Date: Mon Mar 7 09:15:41 2005 Subject: Help on Factors effecting Basidiospore release Message-ID: I'm trying to complete a very small research projest conducted during a field course in algonquin park. I am looking at the time required for basidiomycetes (Clavariaceae in particular) to begin releasing spores after being exposed to rain. My data collection as has many sources of error, but I'm doing my best to understand it. I would like some info on factors effecting spore release, if there has been other work completed on this subject, and any other advice you would like to give me or refer me to. This is not a huge project and is due shortly but any help would be much appreciated. Thank you Jamie Bennett U of waterloo ------------------------------------------------------------------------------ J Bennett From rwinder at PFC.Forestry.CA Thu Oct 27 11:58:07 1994 From: rwinder at PFC.Forestry.CA (Richard Winder) Date: Mon Mar 7 09:15:41 2005 Subject: ** What are yeast? ** References: Message-ID: <1994Oct27.165807.5124@spruce.pfc.forestry.ca> In article , thanh@wwg3.uovs.ac.za writes: > >Hi! >For most of people yeasts are Sac. cerevisiae. >For most of yeastists yeasts are the fungi with single cells stage of >development. But Mucor spp. can give us such kind of cells in condition >without O2 as well. >So, how do you think about "what are yeast" and "their origin"? > > origin of yeasts: > / ... / fungi1 / yeasts > Fungi - ... or yeast - fungi2 or X - fungi > \ yeasts \ fungi... \ ... > > or ... I don't know if you can equate yeast cells with a hypothetical unicellular protist/alagal ancestor- perhaps you can in the `lower' taxons. Many higher filamentous fungi also produce yeast-like growth in liquid culture- whether this common adaptation is homologous or analagous to a reversion back to an ancestral form or not is an interesting question (essentially, is there recapitulation of evolution in fungi, as there seems to be in mammalian embryogenesis?), but I don't know if we have all the information we need to answer it. Some claim that mycelia have developed analogously in the chytrid line and the oomycete line from different ancestral precursors- why not the analagous development of yeasty propagules as well (particularly in the deuteromycetes)? On the other hand, unicellular vegetative structures seem to be important enough to be conserved throughout fungal evolution (I'm thinking of germinated spores, and the ability of single-celled hyphal fragments to propagate). I'm not an expert in the field of fungal phylogeny- it would be interesting to hear what the lasted story on all of this is. -RSW RICHARD WINDER Title: Research Scientist Canadian Forest Service Phone: (604) 363-0773 Victoria, B.C. Internet: RWINDER@A1.PFC.Forestry.CA From herbswa at aol.com Fri Oct 28 12:14:08 1994 From: herbswa at aol.com (Herbs WA) Date: Mon Mar 7 09:15:41 2005 Subject: Polypores: poisonous Message-ID: <38rbh0$r04@newsbf01.news.aol.com> By polypores, I assume that you mean boletes and all their cousins. The true polypores are all woody, inedible things that are usually found on trees and stumps-- the conks and the bracket fungi. As far as boletes are concerned, the only ones that are trully poisonous have red pores-- there was actaully a death in Oregon from one of these only 10 days ago. (It is the first case I have ever heard of) From REDHEADS at NCCCOT.AGR.CA Fri Oct 28 11:31:55 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:41 2005 Subject: Eliminating Mushrooms Message-ID: <01HIT619NIZM004L6I@GW.AGR.CA> I am frequently asked how to get rid of mushrooms, particularly from lawns. Depending upon which kinds of mushrooms the answer may be obvious or not at all obvious. Mushrooms on buried wood or rotting roots may be eliminated by removing the substrate (often easier said than done). Mushroom which are mycorrhizal come with the associated tree or shrub usually planted nearby and the cute may be more drastic than the "problem". However, for the other categories, the soil or thatch inhabiting species, it is sometimes recommended that changing the soil pH might help, for example by liming the soil. Of course what might happen is that one mushroom flora might be exchanged for another. My question to the mycology net is this - Does anyone know of a type of commercially available treatment for soils such as lawns, or gardens, which might be sprinkled on (liquid or powder form) which will either kill most basidiomycetes or at least suppress fruiting? It would need to be legal in Canada but I am open to learning what might be used in other countries. If you know of such a treatment perhaps you could let us know what other effects it might have, i.e. it kills mycorrhizal plants, is toxic to humans, dogs, leaves residues which last decades, that sort of thing. Thanks. I look forward to a discussion on this often requested remedy. Oh yes - I normally like mushrooms and do not consider them to be pests just because they fruit on lawns, but each person has their own views. Scott Redhead Agriculture and Agri-Food Canada From REDHEADS at NCCCOT.AGR.CA Fri Oct 28 11:14:34 1994 From: REDHEADS at NCCCOT.AGR.CA (REDHEADS@NCCCOT.AGR.CA) Date: Mon Mar 7 09:15:41 2005 Subject: Peter Bedker please contact me. Message-ID: <01HIT5CRDDKY004X7U@GW.AGR.CA> Hi Peter - Please contact me again. I lost your email address. Thanks . Scott Redhead From bss097 at clss1.bangor.ac.uk Fri Oct 28 07:56:04 1994 From: bss097 at clss1.bangor.ac.uk (Dr. G.W.Griffith) Date: Mon Mar 7 09:15:41 2005 Subject: Oomycete plasmids?? Message-ID: <38qsd4$sq@mserv1.dl.ac.uk> Hiya, Have any oomycetologists out there come across plasmids in any Phytophthora spp. (or other oomycete spp.)? I haven't come across any reports in the literature but I haven't searched exhaustively. Any pointers would be welcome. Gareth Wyn Griffith University of Wales Bangor, Gwynedd LL57 2UW From slim at OLYMPUS.NET Fri Oct 28 23:39:17 1994 From: slim at OLYMPUS.NET (EDThielk2) Date: Mon Mar 7 09:15:41 2005 Subject: Exploring fungus culture Message-ID: Hi, I am a high school biology teacher, and an amateur field mycologist. Every year I have students collect mushrooms, make observations on local diversity (Olympic Peninsula, Washington) and view spores under the microscope, etc. This year, while sampling the campus for bacteria, and growing some cultures, several agar plates sprouted fungus. My question is - if I innoculate nutrient agar with spores from mushrooms collected in the field, could I expect some hyphae to grow? I can't recall at the moment the constituents of the agar I am using up at school. I believe that it is a yeast or beef extract with nutrient gelatin added. All I really want at this point if for my students to see another stage in the cycle of the mushrooms they see in their yards and the forests around here. If the answer to this question is too complicated to do email, perhaps a reference would work. Thanks David Thielk Port Townsend, WA slim@pt.olympus.net From lorelei at U.WASHINGTON.EDU Fri Oct 28 22:34:52 1994 From: lorelei at U.WASHINGTON.EDU (Lorelei Norvell) Date: Mon Mar 7 09:15:41 2005 Subject: Polypores: poisonous References: <38rbh0$r04@newsbf01.news.aol.com> Message-ID: On 28 Oct 1994, Herbs WA wrote: > By polypores, I assume that you mean boletes and all their cousins. The > true polypores are all woody, inedible things that are usually found on > trees and stumps-- the conks and the bracket fungi. As far as boletes are > concerned, the only ones that are trully poisonous have red pores-- there > was actaully a death in Oregon from one of these only 10 days ago. (It is > the first case I have ever heard of) > > Apparently the poisoning involved a couple who ate Boletus pulcherrimus (formerly known as eastwoodiae). The wife recovered, the husband died. Would have to check with the Toxicology Chairman of the Oregon Myc9ological society to be certain, but I believe that the husband already had a medical history with other complications. Both were quite ill, however. Normally, we don't really consider Boletes to be Polypores..... Lorelei From stamets1 at aol.com Sat Oct 29 11:54:05 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:41 2005 Subject: Polypores: poisonous References: <38rbh0$r04@newsbf01.news.aol.com> Message-ID: <38tund$kfp@newsbf01.news.aol.com> In article <38rbh0$r04@newsbf01.news.aol.com>, herbswa@aol.com (Herbs WA) writes: Thanks but I do not consider Boletes to be true "polypores". They have pores, but not in the same sense as is commonly accepted. what species of Boletus was responsible for the poisoning? paul From stamets1 at aol.com Sat Oct 29 11:52:01 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:41 2005 Subject: Eliminating Mushrooms References: <01HIT619NIZM004L6I@GW.AGR.CA> Message-ID: <38tujh$ke7@newsbf01.news.aol.com> In article <01HIT619NIZM004L6I@GW.AGR.CA>, REDHEADS@NCCCOT.AGR.CA writes: I am also asked this with alarming frequency. My standard response isto recommend a credentialed psychotherapist so that the percieved victim of mushroom invasion can more deeply examine their fears/intolerance of fungi. At the end of one's life, the fungi will win anyhow. (This statement seems particularly disturbing with those not at peace with their own mortality.) Besides removing the substrate, a layer of concrete also works. Otherwise I know of no solution short of ecological catastrophe. paul stamets From stamets1 at aol.com Sat Oct 29 12:01:01 1994 From: stamets1 at aol.com (Stamets 1) Date: Mon Mar 7 09:15:41 2005 Subject: Exploring fungus culture References: Message-ID: <38tv4d$kj5@newsbf01.news.aol.com> In article , slim@OLYMPUS.NET (EDThielk2) writes: If the spores are fresh, and for instance, if they are from Oyster mushrooms, the probability of your getting Oyster mushroom cultures is very high. If 1/20 gram of gentamycin sulfate is added to your agar media, most bacteria will be inhibited. Always leave several plates uninoculated so that you can determine if any contamination is intrinsic to the media prep. process or the transfer technique. In observing mycologists doing isolates over the past twenty years, I have been amazed at how many try to do isolations without a laminar flow hood, in rooms where dozens of sporulating mushrooms are pumping germ plasm into the air. This circumstance, I believe, has led to dozens of mislabelled cultures which are then passed to others with some air of certainty. Good luck. And be prepared surprises. paul stamets From gash at u.washington.edu Sun Oct 30 12:02:04 1994 From: gash at u.washington.edu (Chris Schadt) Date: Mon Mar 7 09:15:41 2005 Subject: Cordyceps capitata Message-ID: I am taking an general mycology course this quarter and we recently ran across this "truffle eating" fungus on the Olympic penninsula of Washington State. Arora's book "Mushrooms Demystified" says it might possibly be worth eating, but he could find no literature on it. My lab TA said he had read that it or another Cordyceps and the truffle host were used ritually (in Australia?). My question for the newsgroup is: does anybody know of or have access to any references on the subject and Does the parasitic fungus or the truffle or the fungus and truffle or neither have hallucinogenic properties? Thanks for any responses. ------------------------------------------------------------------------- /\ Christopher W. Schadt | "I never knew how soothing /\ /||\ 5249 17th Ave NE #6 | trees are--many trees and /||\ //||\\ . Seattle, WA 98105 | patches of open sunlight, //||\\ . //||\\/|\ (206) 517-5840 | and tree presences--it is //||\\ /|\ //||\\/|\ gash@u.washington.edu | almost like having another //||\\ /|\ //||\\/|\ Botany Undergraduate | being." || | || | Univ. of Washington | -D.H.Lawerence ------------------------------------------------------------------------- From ndpm at aol.com Sun Oct 30 21:27:03 1994 From: ndpm at aol.com (NDPM) Date: Mon Mar 7 09:15:41 2005 Subject: Help on Factors effecting Basidiospore release References: Message-ID: <391kln$kej@newsbf01.news.aol.com> In article , JGBENNET@SCIENCE.watstar.uwaterloo.ca (J Bennett) writes: Look for "Researches on Fungi" by Buller (V.1-7). Good luck. From ndpm at aol.com Sun Oct 30 21:30:05 1994 From: ndpm at aol.com (NDPM) Date: Mon Mar 7 09:15:41 2005 Subject: Eliminating Mushrooms References: <01HIT619NIZM004L6I@GW.AGR.CA> Message-ID: <391krd$kg5@newsbf01.news.aol.com> In article <01HIT619NIZM004L6I@GW.AGR.CA>, REDHEADS@NCCCOT.AGR.CA writes: If someone doesn't like a mushroom in the yard they can go and pull it up and throw it away... Phil McIntosh. NDPM@AOL.com From 003768h at axe.acadiau.ca Mon Oct 31 18:37:00 1994 From: 003768h at axe.acadiau.ca (ANDREA HOYT) Date: Mon Mar 7 09:15:41 2005 Subject: Lactarius of the North Message-ID: <003768h.1.2EB57F9C@axe.acadiau.ca> I'm doing a project (undergraduate) on the genus Lactarius of Nova Scotia, Canada this year, and would appreciate any information or advise on literature, or recent studies. Next year, I'll be doing a thesis on the Lactarii of the Yukon, northern British Columbia, and south-eastern Alaska. As far as I have been able to ascertain, no scientific studies have ever been done on this genus for this area. If anyone has any information on Lactarii of the North, I would be very happy to hear from you. Also, if you can suggest books, articles, or recent studies, that would be grand. Thank you very much. - Andrea J. Hoyt Acadia University e-mail address: 003768h@axe.acadiau.ca or snail mail at: ASU Box 6341 Wolfville, N.S. B0P 1X0 Canada