PCR from fungal colonies

Seanna Annis sannis at uoguelph.ca
Fri Oct 7 20:59:57 EST 1994

J Kennedy (mb1jxk at silver.shef.ac.uk) wrote:
: I am going to be doing some PCR on fungal colonies from
: plates. As I will be screening a lot of colonies 
: (from a cross of Aspergillus nidulans) I am looking for 
: a quick and reliable method. Any suggestions would be 
: welcome.

: j.kennedy at sheffield.ac.uk

In our lab we have been using a boiling prep method, very little time 
involved. I believe one of the methods can be found in Biotechniques.  I 
have not used the method myself, but many people in our lab have, and it 
usually worked well.  Good luck. Seanna sannis at uoguelph.ca

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