Isolation of Geotrichum sp.'s spores

C.C.Chen r3603207 at CC.NTU.EDU.TW
Tue Jul 4 07:59:17 EST 1995

I use malt extract agar slant to cultivate  Geotrichum. After 3 
days of growth,
I use 1% triton soln to wash the slant getting the spore suspension.
But when I cultivate it in the ME agar again. I find that only 5 spores
are germinative. I use LM to detecte it finding that there is many spores
cross-link together.
How can I getting the spores suspension?
And how can I getting the mono-spore ?

THANK YOU!!!!!!!

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