xyzzyx at aimnet.com
Thu Jul 20 00:47:34 EST 1995
In article <3ucei6$e4d at nexus.uiowa.edu>, michael-clark at uiowa.edu says...
> Sorry if this seems like a stupid question, but I've recently taken a
>tissue culture from a black morel, and the mycelium looks very
>different from other mushroom mycelium I've seen (C. comatus, A. brun.,
>S. rugoso-annulata). It is much less dense, whitish brown, and appears
>to grow into the agar as well as on top of it. My media is standard
>malt-extract agar supplimented with yeast extract with ampicillin
>(200mg/liter of agar) added to suppress bacterial contamination. Is
>this appearance normal for morel mycelium, and are there any
>recommendations on agar media? Thanks.
>michael-clark at uiowa.edu
your description sounds very much like Morchella. I have a protocol
for fruiting morels that you may be interested in (although it is amazingly
complex, it is allegedly effective). I obtained it from Fred Stevens of the
San Francisco Mycological Society several years ago, when I took a mushroom
cultivation course taught by him.
I actually typed the whole thing in and tried to send it to you last
night, but my mailer freaked out and crashed my PC just as I went to send it.
The gist of it is that you grow up the mycelia in a good growth
medium, refrigerate, soak, case, then fruit. There is a fair amount of
humidity, CO2, and illumination manipulation involved.
If you are interested, please send me an Email and I will be happy to
type it up and send it to you.
Email: xyzzyx at aimnet.com
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