michael-clark at uiowa.edu
Thu Jul 20 18:14:58 EST 1995
I did EMS mutagenesis of yeast when I was an undergrad. After the
cells had incubated in EMS, the EMS was destroyed by adding the cells
(with EMS) to 5% sodium thiosulfate. The cells were spun down and the
thiosulfate solution disposed of. I believe that we added this
solution to a little more 5% Na thiosulfate and let it sit in the fume
hood for a week or two. Consult your health protection office on this,
but as I recall, we disposed of it as regular chemical waste (i.e. as
thiosulfate since the EMS was destroyed).
BTW: I echo the comments about doing this in a fume hood, and defintely
wear double gloves. EMS is nasty stuff.
University of Iowa
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