help in choosing a microscope
hlehto at utu.fi
Wed Sep 18 05:56:10 EST 1996
I can tell you about my own short experience. There are several types
of microscopes around. If you want to look at spores, and other minute
details in fungi forget the stereomicroscopes and go for a proper research
microscope. Forget also the 100$ "you get 1000x magnification" toy microscopes.
Some proper (college) student microscopes seem satisfactory too. Make sure
it has also 2 eyepiece tubes, one for each eye (many people would mistake
it for a stereo microscope).
I purchased mine Reichert microscope from a university medical department
for a very modest price and have had it for slightly over a year now.
My Reichert is some 20 years old, but still in very good condition.
It has opened a new world for me.
Ask around and you may find one. The only potetential problem I had was that
spare bulbs were difficult to obtain, but that too is solved now.
I would say that the following are musts for you..
1) Transmitting light *research* (or optically good quality student) microscope2) with objectives >60x, preferably 90x or 100x and NA (numeric aperture)
at least about 0.9, prefarably 1.2-1.3.
3) Eye-piece magnification 8x, 10x or 16x.
4) Minimum combination should give >600x magnification. Combinations
that satisfy the mimimum criterion are 60x and 10x or 100x and 8x,
the latter being better.
The combination I'm using is 16x eyepiece, with a self constructed (and
calibrated) scale and a revolver with objectives 4x 10x 40x and
100x (N.A. 1.25) (thus giving me an effective magnification of 64x,
160x, 640x and 1600x).
5) Oil (or water)immersion capability. For objectives 90x and larger you
need to use oil immersion to get all the resolution out of your
6) TEST THE MICROSCOPE. Take it to a mycologist. Try is yourself on spores
and check if you can see the details of the spores or if you can detect
bacteria or algae in stangant water (back yard pond, flower vase).
I never saw a bacterium with my "get 1000x microscope" toy type
microscope, but I can now see them easily with my proper microscope even
with 160x magnification!
7) Good stock of supplies. Cover glasses etc.
8) For studying fungi you need also chemicals, Melzer's reagent and others.
That's to start with...
Harry J Lehto
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