Preservation of cultures?
Eric.Wilk at tufts.edu
Mon Mar 24 06:14:37 EST 2003
This came from chemistrystore.com:
Synonyms: Paraffin oil; Heat-treating oil; Hydraulic oil; Cable oil;
Lubricating oil; Oil mist, refined mineral; mineral oil mist; oil mist,
mineral, severely refined; Uvasol; Paraffin oils; Mineral oil
hydrocarbon solvent (petroleum); Mineral oil (saturated parrafin oil);
oil, petroleum; Nujol; liquid paraffin; white mineral oil; clearteck;
drakeol; hevyteck; filtrawhite; frigol; kremol; kaydol; alboline;
paroleine; Saxol; adepsine oil; glymol; lignite oil; blandol white
mineral oil; carnea 21; Ervol; gloria; hydrocarbon oils; peneteck;
primol; triona b; blandlube; crystosol; Molol; protopet; bayol f;
crystol 325; fonoline; bayol 55; kondremul; neo-cultol; oil mist;
penreco; perfecta; petrogalar; primol 355; primol d; tech pet f;
Petroleum hydrocarbons; jute batching oil; paraffin oil (class);
petrolatum, liquid; white oil; Mineral oil, aromatic; Mineral oil,
paraffinic; Mineral Seal Oil; Electrical Insulating Oil; OIL MIST,
MINERAL (MINERAL OIL)
It sells for $11 / gal -- I don't see how this is so expensive.
BTW -- make sure to sterilize it first!!
"Bertil Lindstrom (Bertil Lindstrom)" wrote:
> So, is it maybe "castor oil" or something similar?
> On 20 Mar 2003 09:18:29 -0000, please_post at noreply.com wrote:
> >Bertil wrote:
> >> Hi out there!
> >> How do I preserve the cultures for future use?
> >> I have read about deepfreezing in liquid nitrogen, but that is out of
> >> the question -:)
> >> Somewhere else (Stamets ?) talks about storing cultures in "mineral
> >> oil".
> >> Now what excatly is "mineral oil"? Ordinary motor oil or cooking oil
> >> or some special oil?
> >You can find it in the pharmacy section
> >of almost any grocer, near the hydrogen peroxide, rubbing alcohol, witch
> >hazel, etc. I think, medically speaking, it's used as a laxative.
> >> Is there any other cheep ways of storing live cultures?
> >Mineral oil is the cheap route. Grow the strains you want to
> >preserve in MEA-filled culture tubes, then add enough minerail oil to
> >cover them. Place the culture tubes in cold storage at just over
> >freezing (I think 36F is recommended.) You may be able to keep them
> >in the fridge, for example. If you're going to do this somewhere that
> >isn't perfectly temperature-regulated, though, make a few backups and
> >distribute them to other people for holding in their fridges as well.
> >to that if you don't hold it ope
> >> /Bertil
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