Substraction Libraries?

Humberto Oritz-Zuazaga zuazaga at ucunix.san.uc.edu
Sat May 11 14:51:29 EST 1991

I am posting this note on behalf of my wife, who reads this group over
my shoulder.  Replies to me or the net, etc.

I am thinking about determining what neuronal genes are induced
following excitation, besides the immediate-early genes.  I have
been told that substraction libraries may be of help but frankly
I have no idea of what they entail and how this procedure will help
me.  If anyone has any experience with substraction libraries I
would appreciate information on their applications and how they
are actually done.

Sandra Pena
Molecular Neurotoxicology Program
Dept. of Environmental Health
University of Cincinnati
Humberto Ortiz-Zuazaga                               University of Cincinnati
                                                      Physiology & Biophysics
zuazaga at ucunix.san.uc.edu                            Cincinnati OH 45267-0576

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