IUBio

Micropipette pulling question

taaustin at vaxb.acs.unt.edu taaustin at vaxb.acs.unt.edu
Wed Apr 1 11:08:06 EST 1992


I am using a Sutter P 87 micropipette puller to make microelectrodes for
intracellular studies.  I am consistently getting an uneven narrowing or "neck"
about 10 to 20 microns from the tip.  Is this detrimental, what causes it, and
how can I get rid of it, if necessary?

Thanks 



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