Controls for fixation and Antibodies

taaustin at vaxb.acs.unt.edu taaustin at vaxb.acs.unt.edu
Thu Aug 6 23:07:16 EST 1992

Looking at your protocol I had to wonder, do you flush the circulatory
system first with normal saline?  Your fixation looks similar to mine
except for that.  By the way, I -regularly- perfuse P0 (day of birth)
rats intracardially using gravity perfusion.  These always turn out
quite well for my in-situ hybridzations.
Terry A Austin
Dept of Biological Sciences
University of North Texas
Denton, TX
taaustin at vaxb.acs.unt.edu

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