In article <1992Aug5.144701.21487 at wam.umd.edu>, wmsaidel at wam.umd.edu (William M. Saidel) writes...
>In article <13824 at gazette.bcm.tmc.edu> rchafetz at cephalo.neusc.bcm.tmc.edu (Ross S. Chafetz) writes:
>>>>>>Question
>>>> I'm doing some antibody staining, using ABC
>>(vector kit). The sections (35um) are free floating in wells.
>Sticking sections, in my experience, is due to i) poor fixation and/or
>ii) mold in the storage medium. Tell us more about the conditions you
agreed. also tris-based buffers seem to be worse than phosphate buffers
for stickiness, and the younger the brain, the worse the sticking. i suspect
it's the fixation.
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|Glenn Holm Internet:karuzis at wccf.mit.edu|
|M.I.T Dept. of Brain + Cog. Sci. Bitnet:karuzis at mitwccf |
|Cambridge, MA 02139 "Real Neuroscientists don't do gels!" |
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