In article <22072717515542 at vms2.macc.wisc.edu> BQWANG at MACC.WISC.EDU (Baoqing Wang) writes:
>Dear friends:
>I am trying to use Lucifer Yellow (LY) filled glasselectrode to do
>intracellular recording, and then inject LY into the cell to label
>it. I find that it's difficult to keep the cell for a long time.
>Would anyone please share your experience with me? Any suggestion
>would be appreciated. Thank you in advance.
It's been a while, but I might be able to offer suggestions. But please givee
more information:
What type of cell (what animal?)la
What size of cell?
What size electrode tip (resistance in 3 M KCl for example)?
Pressure or iontophoresis for injecting Lucifer?WQ
This information may assist others that could helpe
Good lucj
