I am a Ph.D. student and will graduate in summer of 1992. I am looking for a
job or a postdoctoral position in the fields of life science. The following
is my CV.
CURRICULUM VITAE
Jinhe Li
Department of Biochemistry & Molecular Biology
State University of New York
Health Science Center
Syracuse, NY 13210
(315) 646-8734
LIJ at snysyrv1.bitnet
EDUCATION
1987-present. Ph.D. Candidate, Department of Biochemistry &
Molecular Biology, State University of New York
Health Science Center, Syracuse, New York.
1983-1986 M.S. in Medicine, Department of Virology, National
Institute for the Control of Pharmaceutical and
Biological Products, Beijing.
1977-1979 Student, the School of Health Related Professions
Chinese Academy of Medical Sciences, Beijing.
AWARDS AND HONORS
Chinese Ministry of Public Health Award second prize, Beijing,
1985.
Chinese Ministry of Public Health Award third prize, Beijing,
1986.
Chinese Academy of Science Young Scientist Award, Beijing, 1987.
Chinese Ministry of Public Health Award third prize, Beijing,
1989.
SUNY-HSC Alpha-Omega-Alpha Research Forum Award Second Place,
Syracuse, 1989.
Personal Data
Date of birth: 02/01/59
Place of Birth: Beijing, China
Sex: Male
Home Address: 500 Harrison Street, Apt. 1905, Syracuse, NY
13202 (315) 426-7875
RESEARCH EXPERIENCE
1987-present Ph.D. Student, Department of Biochemistry and
Molecular Biology, SUNY-Health Science Center at
Syracuse, New York.
Subject of Research: Regulation of cell growth and
differentiation by interferon (IFN):identification
and characterization of a cellular regulatory RNA
(R-RNA) which specifically activates IFN induced
double-stranded RNA dependent protein kinase in
3T3-F442A cells.
Techniques Applied: protein kinase assay, cDNA
library construction and screening, DNA
sequencing, Southern/Northern/Western blot
analysis, RNA-protein band shift assays, PCR,
computer-aimed analysis of sequencing data
(Wisconsin Genetic Computer Group).
1986-1987 Research Associate, Department of Virology,
National Institute for the Control of
Pharmaceutical and Biological Products, Beijing.
Subject of Research: Preparation of monoclonal
antibody to cytomegalovirus.
Techniques Applied: hybridoma technique, protein
purification, cell culture, immunochemistry,
serological test.
1983-1986 M.S. student, Department of virology, National
Institute for the Control of Pharmaceutical and
Biological Products, Beijing.
Subject of Research: Development of an anti-m
chain CAPTURE immunoassay of rubella and measles
virus specific IgM for rapid diagnosis of the
primary viral infections.
Techniques Applied: ELISA, radioimmunoassay
(RIA), enzymatic and radioactive labelling of
IgG, protein purification, FPLC, HPLC, generation
of polyclonal antibody, cell culture, serological
test, statistical epidemiology analysis.
1979-1983 Research Technician, Department of Virology,
Institute of Antibiotics Chinese Academy of
Medical Science, Beijing.
Subject of Research: Study of in vivo and in
vitro antiviral chemotherapy.
Techniques Applied: cell cultures (primary and
cell line) and animal models (mouse, guinea pig
and rabbit) for viral infections (HSV-I/II, VSV,
influenza, JEV and EBV) and antiviral
treatments, in vivo interferon assay.
SUMMARY OF RESEARCH ACTIVITIES
IDENTIFICATION AND CHARACTERIZATION OF A CELLULAR REGULATORY RNA
WHICH SPECIFICALLY ACTIVATES THE INTERFERON (IFN) INDUCED
DOUBLE-STRANDED RNA DEPENDENT PROTEIN KINASE (DSI) IN 3T3-F442A
CELLS. (1987-present, Ph.D. student)
The double stranded RNA (dsRNA)-dependent protein
kinase (dsI) is an IFN-induced enzyme which, once activated by
dsRNA, catalyzes the phosphorylation of the a-subunit of the
eukaryotic initiation factor-2 (eIF-2a) resulting in a rapid
inhibition of protein synthesis. dsI has been implicated as
mediating in part the anti-viral and anti-proliferative effects
of IFN. It may also be important in the regulation of growth and
differentiation of some cells. 3T3-F442A cells spontaneously
produce and secrete IFN and exhibit a pattern of dsI
phosphorylation which is related to specific stages of cell
growth and differentiation from fibroblast to adipocyte. dsI,
after induction by autocrine IFN, is phosphorylated in vivo and
is active in reducing total cellular protein synthesis which we
believe is necessary for the cell differentiation. This occurs
in the absence of viral infection or upon addition of dsRNA
which is required for the dsI activation (phosphorylation). My
project is to determine the mechanism by which the kinase is
activated in the cells. As the first time, I isolated a novel
cellular regulatory RNA (R-RNA) which specifically activates the
kinase in the cells. The partial cDNA of the R-RNA has been
cloned by testing the transcript in kinase assay and
characterized as an unique poly[A]+ RNA with double-stranded
structures which bind to and activate the kinase. I am now
cloning the full-length cDNA by constructing and screening the
cDNA library by means of PCR and hybridization, and identify the
double-stranded region within the R-RNA molecule which interact
with dsI by means of liquid hybridization and RNA band shift
assay.
ANTI-m CHAIN CAPTURE IMMUNOASSAY OF RUBELLA AND MEASLES VIRUS
SPECIFIC IGM FOR RAPID DIAGNOSIS OF THE PRIMARY VIRAL INFECTIONS
(1983-1986, M.S. Student)
In the most viral infections IgM antibody is the
primary immune response and is maintained shortly. So the
detection of specific IgM could demonstrate a primary viral
infection, which is especially important for the diagnosis of
rubella and measles: primary rubella infection of woman early in
pregnancy can lead Congenital Rubella Syndrome (CRS); effective
distinguish between the primary and secondary measles infection
after inoculation of live measles vaccine is the key for
monitoring the programmed immunization. I developed a CAPTURE
immunoassay using anti-m chain antibody as the solid phase
capture antibody and using enzyme- or radio- labeled anti-viral
monoclonal antibody for specific detection (ELISA or RIA).
Comparing to the standard serological test, 2-ME/SPA treatment
test, IgM isolation and indirect immunoassay, the CAPTURE
immunoassay was shown the best in terms of specificity,
sensitivity, simplicity and time saving. The ELISA system
established for rubella diagnosis has been developed as a
diagnostic kit which is comparable to the one produced by Abbott
and is now the only one commercially available in China and
applied nationwide. This study was honored the Chinese Ministry
of Public Health Award Third Prize in 1989.
IN VIVO AND IN VITRO ANTIVIRAL CHEMOTHERAPY (1979-1983,
Research Technician)
I was involved in the study of the in vivo and in
vitro antiviral effect of a series of chemical compounds and
the components of Chinese traditional medicinal herbs. I
established several experimental procedures used on a daily
basis in the lab such as: microplate cell culture system for
different cell stains or lines used for different in vitro viral
infections including herpes simplex virus type I and II (HSV-I
and II), vesicular stomatitis virus (VSV), and for antiviral
treatment; in vivo interferon (IFN) assay; animal models for in
vivo viral infections and antiviral treatment, including rabbit
eye model and Guinea pig skin model for HSV-I virus, and mouse
vagina model for HSV-II virus. Using these m
