Does anyone know of an example of a reporter construct that can not be
regulated in qualitatively the same manner in transient assays as in assays
of a stably transfected line? Or vice versa for that matter?
I have a CAT reporter that I'm electroporating into PC12 cells. I am
utterly failing to reproduce results due to membrane depolarization published
with a stable transfection of the same construct. I'm mystified and wondering
whether it's worth pursuing. Any clues welcomed.
E-mail: mhollowa at ccmail.sunysb.edu (mail to freenet is forwarded)