Hi,
I need a little help. I have been doing some work with the
biochemistry of the Acetylcholinesterase of the horseshoe crab (limulus
sp.). I have had a little trouble purifying the enzyme. I have about 5.3
Delta A/min, in a volume of .41 mL. I have 4 such samples. Now the
problem. I have run out of money. I am a concurrent student at both a
public high school(Finally a senior!!) and a Student at Ohio State.
Anyway, I have been running this project, and the bio. dept at OSU won't
give me anymore money. I am on a regional campus, which has few
resources. I have had to purchase a column, the limulus, and of course
chemicals. What else can I do [besides the obvious kinetics (V max; and K
sub M; etc.), that will not consume a whole lot of resources, with what
little enzyme I have. Please respond by email.
Thanks in advance,
Pravin Muniyappa
PS: The bio. dept budget for the fiscal year is $2000, I have spent $400.
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| Pravin Muniyappa |
| The Ohio State University |
|pmuniyap at magnus.acs.ohio-state.edu |
| "I may be a dreamer, but I'm not the only one." -JL |
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