On Thu, 22 Dec 1994 11:02:08 GMT,
elis f. stanley <elis at helix.nih.gov> wrote:
>In article <54420.caudle at irp.nidr.nih.gov> "caudle at irp.nidr.nih.gov"
<caudle at irp.nidr.nih.gov> writes:
>>On 19 Dec 1994 11:31:00 -0800,
>>Fiberman <william at neuro.usc.edu> wrote:
>>>>>>I wish to detect a receptor at the synapses of the mammalian brain. I
>>>separate receptors at the synapse from those inside the cell (ie. those
>>>ER, Golgi, or microsomes). If you know of a reference that talks about
>>>purifying synaptic proteins, please let me know. Thanks!
>>>>Any general biochemistry cookbook has a recipe for isolating synaptosomes.
>>However, hear is a couple of references to a reasonable procedure. Neuron
>>9:929-942, 1992. PNAS 91:3954-3958, 1994.
>>This is the right answer to the wrong question I think - unless the intent
>is to isolate presynaptic receptors. As I understand the post the author
>is after postsynaptic receptors that are inserted into the surface
>do not have any answers but can we try again?
The Neuron paper describes isolating postsynaptic NMDA receptors from
Robert M. Caudle "If I had my life to
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Caudle at yoda.nidr.nih.gov
Caudle at irp.nidr.nih.gov