In article <311sq8$q8d at agate.berkeley.edu>, navajo at uclink2.berkeley.edu
(Wilfred F Denetclaw) wrote:
> Hello,
>> I would like to deliver DiI to somites in developing chick embryos using
> iontophoresis. However, my electrode wires need Ag/AgCL coating. Can
> someone experienced with this process provide me with information on how
> to do this? Thanks a lot.
>> Wilfred
The easy way is to stick your silver wire into freash bleach for about
twenty min. This should be done about once a week because the chloride
does come off with use. A more difficult way is to use electrolysis with
a D (1.5V) battery.
Solution is .01 M HCl and .1 M NaCl. Attach the silver wire to the + and
a ground to the - and place both into the solution. Bubbles will let you
know that it is working. Again leave for about 20 min and repeat once a
week.
Good Luck!]
-Chuck
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