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C-Fos and ANP ?

B. Zhu bz10001 at CUS.CAM.AC.UK
Sat Nov 5 10:21:23 EST 1994

Are there any people here who are doing c-Fos and ANP ( Atrial Natriuretic 
peptide ) or who know their colleagues are doing it. I am using c-fos 
technique to map the central effects of ANP in the brain. As you may 
know, ANP activates cGMP second messenger system to result in 
physiological effects. There is one paper in which author described 
Nitric Oxide ( NO ) activated cGMP to induce c-fos and Jun-B expression 
in PC 12 Cells. I have spent almost half year to try whether ANP can 
induce c-fos or not with different doses ( 250, 500, 1000 and 2000pmol ) 
by i.c.v. But, I failed to find any significant c-Fos expression in the 
brain compared with CSF control. I can understand that the c-fos 
expression induced by NO in PC 12 cells can not be used to argue that ANP 
can induce c-fos expression in the brain because It activaed cGMP system. 
But, people did find that stimulation which can induce c-fos in PC 12 
cells always induce c-fos in intact nervous system.

Your help will be highly appreciated if you can let me know that some 
of your colleagues have same experience, or if you can give some comments 
on what I have got, especially the c-fos induction and second messenger 
system, and difference of c-fos ( or other IEGs ) expression in PC 12 
cells and intact nervous system.

You can contact with me through the email ( bz10001 at cus.cam.ac.uk ) or 
send the email to this newsgroup. 

Thanks !

Department of Anatomy
University of Cambridge

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