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Keeping track of cells on circular glass coverslips?

Peter Mulderry mulderry at castle.ed.ac.uk
Sun Nov 6 08:10:05 EST 1994

In article <ladic.301.0019EB69 at cs.ubc.ca>, Lance Ladic writes:

> Does anyone know a way of marking a *circular, glass* coverslip 
(e.g. with 
> grids) that will allow for keeping track of cultured cells

If you need to be able to find individual cells, then the Cellocate 
coverslips from Eppendorf are definitely worth a try. They are of good 
quality glass and I have found that cells grow very well on them. They 
are expensive, but if they're what it takes to make the experiment 
work then I guess they're worth it. One problem I've found with them 
is that the etched grids are not always easy to see under phase 

It _is_ perfectly possible to mark regular coverslips with a diamond 
stylus without breaking them. I do this regularly. Just don't press 
too hard. The slightest scratch is easily visible under 40X 
magnification. The technique is OK for marking a small area of 
"special interest", but you will have to plate the cells at very low 
density if you want to be able to find previously identified 
individuals this way.

Peter Mulderry, 
MRC Brain Metabolism Unit
Edinburgh, Scotland.
mulderry at castle.ed.ac.uk

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