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need ideas for methods to kill off/ablate cells in chick embryo

User7381 user7381 at aol.com
Mon Nov 7 00:29:22 EST 1994


In article <35uun5$nu7 at scunix2.harvard.edu>, mlevin at husc7.harvard.edu
(Michael Levin) writes:

he need ways to kill chicken cells in culture

Well here are some suggestions:

1) if you have a highly specific label as you indicated then use the laser
but watchout - just the heat alone makes this very unreliable 

2) there is a Michagan company that makes a laser device that allows you
to shoot one cell ata a type without excess heat - if you are still
interested when you get this mail I will look it up for you - someone at
Harvard or MIT or MGH must have one

3) sometimes rapidly growing cells are more heat sensitive - so heat shock
might work - but this is more of an art than a science

4) sometimes Rhodamine 123 - a mitochondrial die remains in fast growing
cells and it will either kill them sua sponte or again you can use a laser
or possibly heat

I hope this is helpful.

G. Corey

user7381 at aol.com



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