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separation of vesicles

Richard Gordon gordonr at cc.UManitoba.CA
Thu Nov 24 22:10:44 EST 1994

Dear Pam,

There has been a small amount of literature on the effects of temperature 
gradients across an embryo on its development. That's a much more 
complicated system, but your proposed work might provide some insights 
for it, or vice versa:

Dean, J.L., M.E. Shaw & M.A. Tazelaar (1928). The effect of a temperature 
gradient on the early development of the frog. Br. J. Exp. Biol.  5, 

Gilchrist, F.G. (1928). The effect of a horizontal temperature gradient 
on the development of the egg of the urodele, Triturus torosus. Physiol. 
Zool.  1(2), 231-268. 

Glade, R.W., E.M. Burrill & R.J. Falk (1967). The influence of a 
temperature gradient on bilateral symmetry in Rana pipiens. Growth  31, 

Huxley, J.S. (1927). The modification of development by means of 
temperature gradients. Wilhelm Roux' Arch. Entwicklungsmechanik  112, 

I've collected these old references, but haven't obtained the papers. In 
exchange for the information, if you dig them out, how about sending me a 

Best regards,
-Dick Gordon[Nov24,94]
Dr. Richard Gordon
Professor, Department Of Radiology, University of Manitoba
Room ON104, Health Sciences Centre, 820 Sherbrook Street
Winnipeg, MB R3A 1R9 Canada
Phone: (204) 787-1076,  Fax: (204) 783-8565,  E-mail: GordonR at cc.UManitoba.ca

On 23 Nov 1994 davidson at umbc.edu wrote:

> Hello, I am a graduate student at the University of Maryland. Currently, 
> I am trying to develop a method to separate axonally transported vesicles 
> based on transit rates. I would like to separate fast axonal transport 
> vesicles that translocate in the lower range (ie ~200 mm/day) from those 
> that translocate in the upper range (ie 400mm/day). I have been thinking 
> about using a temperature step gradient. My initial thinking was that 
> maybe by cooling the axons in a gradient fashion I could stop the slower 
> moving vesicles at a higher temp than the faster moveing ones.  However I 
> have not found any literature examining the effect of temperature (ie 
> cold block) on differentially slowing of translocating vesicles. So I am 
> back to square one. Does anyone have any suggestions as to which 
> direction I should go in order to accomplish this? Any help would be 
> greatly appreciated.  My e-mail address is davidson at umbc.edu.
> Thank you
> Pam Davidson  

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