>With great Interest I have read the article "The Prion Connection" in Science, Vol 264, 22 April 1994, by Charles Weismann.
>The article is a summary of the possibility of prion like disease in yeast " The Prion Connection: Now in Yeast."
>This article seems to be a reinforcement of the "Prusiner Model".
>Otherwise there are some articles published by Harash K. Narang, for example:
> Central Nervous Sytem
> Vol 724 of the Annals of the New york Academy of Sciences June 6, 1994
>>" Evidence that homologous ss-DNA is present in Scarpie, Creutzfeldt-Jakob Disease and Bovine Spongioform Encephalopathy.
>Unique virus-like tubulofilamentous particles, termed nemavirus, have been consistently observed in spongioforme encephalopathic brains by electron microscopy in thin sections.
>SDS treatment of unfixed infected brain tissue on grids revealed a twisted fibril core of tubulofilamentous particles. The unmasked fibrils are not artefacts of protease treatment of protein formed in vitro during purification.
>>Treatment with potease and nucleases revealed that each tubule consists of three layers:
>(i) an outer coat of protease-sensitive material;
>(ii) an intermediate layer that is digested by DNase and mung bean nuclease,
>(iii) inner prtotease-resistent protein scarpie-associated fibril.
>>The intermediate layer consist of an unusal palindrom six base TACGTA repeat sequence.
>The length is 1.2 kB.
>The presence of palindromic sequences in the scarpie genome could explain why previous searchers have revealed no evidence for scarpie-specific nucleic acid." (Harash K. Narang)
>>Is there anyone who have got similar results?
>Is it possible by using PCR to reveal the ss-DNA in living Scarpie-infected animals?
>>For a discussion I need some answers.