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Keeping slices alive and kicking

Marc Brande brande at SDSC.EDU
Wed Nov 30 12:35:15 EST 1994

See paper by Stoppini ?. He used interface culture using Costar membrane
inserts to keep slices alive days to weeks. Paper about 1991. 

Marc C. Brande, M.S.       SD3D Email List:3D Imaging 
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On 29 Nov 1994, Heinz Beck wrote:

> I once heard from a friend, who had heard from a friend etc. that there is
> a method for keeping brain slices of hippocampus or cortex alive for a long
> time i.e. about 24 hours. My understanding was this was achieved by substi-
> tuting sachharose for sodium in the extracellular solution during the pre-
> paration but I could not locate an exact source of information or a protocol
> up to now. Anyone have information or protocols about long-term maintenance 
> of brain tissue slices in vitro?
> We have been doing our preparations with a vibratome and cold  high Magnesium -
> Low Calcium ringer. Storage was submerged in standard ACSF at room temparature.
> Measurements were done using a Haas-type interface chamber. This has kept our
> slices viable for about 12 hours.
> As we are beginning to work on human tissue now, I have been thinking about 
> adding some sort of free radical scavenger enzyme to the preparation ringer 
> to possibly lighten the effects of ischemia during explantation of the tissue.
> Has anyone tried doing this in animal preparations?
> Thanks
> Heinz

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