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polylysine buffer

Hannah Dvorak DvorakH at starbase1.caltech.edu
Wed Nov 30 21:54:28 EST 1994

In article <pataky-2911941510090001 at steevlab.generes.ca>,
pataky at bcu.ubc.ca (Dave Pataky) wrote:

> Hey neuron culturists,
> I have started to culture primary neurons from embryonic chick brain, and
> would like to coat the wells with polylysine, then laminin.  I'm looking
> for a recipe for borate buffer for making up the polylysine, as i've heard
> this is the buffer of choice.  Anyone have such a thing, maybe even know
> why use borate buffer?  Any tips or alternatives would be greatly
> appreciated.  Thanks in advance,

We grow sympathetic and hippocampal neurons on polylysine and laminin, and
we just dissolve the polylysine in phosphate-buffered saline (e.g.
Dulbecco's PBS) at 1 mg/mL.

> -- 
> Dave Pataky
> Dept of Zoology, UBC
> pataky at bcu.ubc.ca

Hannah Dvorak                           |
DvorakH at starbase1.caltech.edu           |
Division of Biology 156-29              | Ceci n'est pas un .sig.
California Institute of Technology      |
Pasadena, CA 91125                      |

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