Does anyone out there use DiI and/or DiA for neuronal tracing in fixed
tissue. I am using both and am interested in other peopleÕs methods.
Any chemists outhere how would you speed the diffusion process of these
lipophillic dyes down myelin or axonal membrane? I have tried heat- 37C
and a silicone base for application.
I would be most grateful for ANY suggestions and or comments.
email : estepien at uniwa.uwa.edu.au