David Sokal writes:
> Thanks to everyone who has already helped me with these cells, but I
> wonder is there anyone else out there who can tell me about these cell
> lines, especially with regards to the following:
> i) methods for differentiation
> ii) synapse formation after differentiation
> iii) where I can get some for free, since my grant is very
>> Thanks very much
>> Dave Sokal
> David Sokal
> Department of Human Anatomy and Cell Biology
> Medical School Telephone: +44 (0)115 924 9924 xtn 42717
> University of Nottingham Fax: +44 (0)115 970 9732
> Clifton Boulevard Email:maxsok at unix.ccc.nottingham.ac.uk> Nottingham, NG7 2UH
I know that you can differentiate NT2s with retinoic acid
(RA). 5 weeks of 10micrograms/ml RA seems to work (Pleasure et al,
J. Neurosci. Res. 35, 585-602, 1993).
We bought our NT2s from Stratagene...
While we're on the topic of NT2s, do you or anyone else know if
these cells express catalase? I would suspect they do, but
have been having trouble detecting any in my assays.
David S. Cassarino "I wanted to live deep and suck out the
MSTP, Neuroscience marrow of life...if it were sublime
UVA School of Medicine to know it by experience."
dsc9w at virginia.edu -Thoreau