On Tue, 30 Jan 1996, Mr D.J.A. Wilson wrote:
> I am trying to study expression of a protein in vitro on chick neurons but I
> haven't been able to find many reliable culture protocols so if anyone out
> there is culturing embryonic or adult chick neurons from any CNS/PNS area
> please drop me a line.
>> David Wilson
>>I have some experience with chick ciliary ganglion neuronal cultures.
I have had the most success with protocols similar to those described
in Nishi and Berg, PNAS, 1977 with some modifications.
Instead of plyornithine or collagen, I use Matrigel
to coat the culture dishes. This seems to help immensely in
maintaining healthy, robust cells and neurites for prolonged
Gail Perez > >