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Patch Clamping Smooth Muscle?

Scott Hancock shancock at morgan.ucs.mun.ca
Tue Feb 13 14:12:24 EST 1996

I'm trying to do a whole cell patch clamp study on smooth muscle cells 
isolated from rat middle cerebral artery. However the cells when 
isolated, have a very inelastic, noncompliant membrane, and I have been 
having trouble getting good seals. Can anybody suggest a reason for this 
problem (solution osmolarity ?) ?

 Scott Hancock 4330 
faculty of medicine Health Sciences Center Memorial University of NFLD
"knowledge is the spice of life but ignorance is bliss."

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