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HELP-A2B5 and GalC immunofluorecence

Andrew K. Groves grovesa at starbase1.caltech.edu
Thu Feb 15 12:42:50 EST 1996

In article <4fvc63$ef0 at news.univ-angers.fr>, Didier Wion
<Didier.Wion at angers.inserm.fr> wrote:

> Hello!
> I am looking for an efficient fixation protocol which can be used to 
> detect the A2B5 and GalC antigens in adherant cell cultures.
> Many thanks

4% Paraformaldehyde for 10 minutes. Wash in PBS. Block with your favourite
serum but use no detergents.
You can also use these antibodies to stain cells live.

Andy Groves
Division of Biology, 216-76
California Institute of Technology

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