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HELP-A2B5 and GalC immunofluorecence

Andrew K. Groves grovesa at starbase1.caltech.edu
Thu Feb 15 12:42:50 EST 1996


In article <4fvc63$ef0 at news.univ-angers.fr>, Didier Wion
<Didier.Wion at angers.inserm.fr> wrote:

> Hello!
> I am looking for an efficient fixation protocol which can be used to 
> detect the A2B5 and GalC antigens in adherant cell cultures.
> Many thanks

4% Paraformaldehyde for 10 minutes. Wash in PBS. Block with your favourite
serum but use no detergents.
 
You can also use these antibodies to stain cells live.

-- 
Andy Groves
Division of Biology, 216-76
California Institute of Technology



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