In article <4fvc63$ef0 at news.univ-angers.fr>, Didier Wion
<Didier.Wion at angers.inserm.fr> wrote:
> I am looking for an efficient fixation protocol which can be used to
> detect the A2B5 and GalC antigens in adherant cell cultures.
> Many thanks
4% Paraformaldehyde for 10 minutes. Wash in PBS. Block with your favourite
serum but use no detergents.
You can also use these antibodies to stain cells live.
Division of Biology, 216-76
California Institute of Technology