I want to mark an extracellular recording site at the end of an experiment via
elctrochemical deposition of Fe ions from the tip of the elctrode (a fine
In my old neurobiology days I recall this was a fairly standard method, but I can't
seem to bring up any references / handbooks that detail the procedures. I'm
interested in general current settings for 'el zappo' and well as gentle
(structure-preserving) markings, and the make up and protocols for developing the
colour (was it with K ferricyanide?, a Prussion blue reaction I think).
Also, can it be done in both whole mounts (cleared or not) and sections?
Any tips, pointers welcomed.