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Anyone know the method for marking (steel) electrode site electrochemically (Prussian Blue?)?

David Parsons dparsons at med.unc.edu
Fri Feb 23 10:28:44 EST 1996


I want to mark an extracellular recording site at the end of an experiment via 
elctrochemical deposition of Fe ions from the tip of the elctrode (a fine 

In my old neurobiology days I recall this was a fairly standard method, but I can't 
seem to bring up any references / handbooks that detail the procedures.  I'm 
interested in general current settings for 'el zappo' and well as gentle 
(structure-preserving) markings, and the make up and protocols for developing the 
colour (was it with K ferricyanide?, a Prussion blue reaction I think).   

Also, can it be done in both whole mounts (cleared or not) and sections? 

Any tips, pointers welcomed. 


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