I have started to use the list Medical Systame loose patch clamp and I am
having some techincal difficulties. Nobody in our lab has used the
amplifier before, and so we're playing like monkies to get the thing to
work. ( I'm using pClamp 5.5.1 to and pClamp interface to deliver voltage
For example, When i make a seal, and try to turn the compensation on, the
potentiometer always turns back to zero! I don't know why!
Also, I can't record anything unless triggering, pulses, and Rs are all
turned off. Otherwwise i just record a whole bunch of square waves.
I've read the bible chapter in "Single Channel Recording" but it doesn't
help with anything practical. It's good, but mostly all theoretical. I
would be grateful if somebody could give me some technical help, direct me
to somebody can, or tell me where to find "loose patch clamp for dummies".
Mark Fry ribit @..@
4th floor BMS Fac of Med. \ (----)
Memorial University of NFLD ( >**< )
A1B 3V6 ^^^""""^^^
wmfry at plato.ucs.mun.ca (Xenopus)