>> Robert L. Hoskins wrote:
>> I've stained some brain sections with thionin, and, after coverslipping
>>the slides, the stain appears to have faded considerably. Anyone have a
>>clue as to why this might happen? Can I remove the coverslips with xylene
>>and re-stain the sections?
I principle, yes. It's a slow business, and there's a risk of
damaging the sections. Possibly your thionin was not the real
thing. There's plenty around that isn't. Make sure your thionin
has a Biological Stain Commission certification sticker on the
bottle of powder.
John A. Kiernan
Department of Anatomy
Univ. of Western Ontario
LONDON, Canada N6A 5C1
e-mail: kiernan at uwo.ca