I have no problem in clearly detecting apototic cells by using The "Apoptag
kit" from ONCOR (the protocol is available in internet).
Goog luck.
Hassan Bousbaa, Porto university.
Brian Scott <brians at interlog.com> escreveu no artigo
<356F8E23.C311C1B1 at interlog.com>...
> I'd like to know if there is a recommended way to detect apoptotic cells
in rat
> brain. I'd like to be able to identify apoptotic cells in fixed slices
for
> double labelling with cell type markers but I've heard that there are
problems
> interpreting the TUNEL technique. I've asked some companies about this
and they
> still recommend these DNA fragmentation detection kits. In the apoptosis
> research community is there a preferred method?
>> Please respond by email.
>> Thanks.
>> --
> Brian Scott | Department of Physiology
>brians at interlog.com | University of Toronto, Canada
> =====================================================
>
