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Mouse oligodendrocytes

F. Frank LeFever flefever at ix.netcom.com
Mon Apr 19 19:45:46 EST 1999


In <371B0332.B9EDFB1A at uni-konstanz.de> Bernadett.Simon at uni-konstanz.de
(Berna) writes: 
>
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>Hi!
>As I am working with murine oligodendrocytes for some time now, I'm
>interested to find persons with the same topic. What are your
>experiences with the isolation procedure and the cultivation?
>Are there so few articles about those cells, because of the
difficulties
>in their cultivation?
>I try to study the cell death of those cells, induced by cytokines
that
>coud be relevant in MS. My cells  don't die from inflammatory
cytokines.
>Is there any explanation around?
>
>Thanks for any reply!
>Bernadett
>

Have you tried seeding your cultures with a few mast cells before
challenge by cytokines?  (What cytokines? IL-1 and TNF?)

F. Frank LeFever, Ph.D.
New York Neuropsychology Group



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