We are studying oxidative stress on cells using flow cytometry after
fixation and addition of H2O2 as a positive control for oxidative stress
but no success. Tell now we couldn't see an effect on cells treated with
H2O2. If you have any ideas, suggestions or protocols how to induce and
measure oxidative stress using H2O2 and flow cytometry or other methods
please let me know.
Thank you for your help.