Please, can anyone advise or suggest alternative approaches.
I am investigating the effects of general anesthetics on the fast transport
of neurotransmitter containing vesicles in axons and the pre-synapse. Is
there a way to fluorescently label either the neurotransmitter (glutamate)
or some component of vesicular membrane so that active transport can be
recorded in real-time using confocal microscopy?
Alternatively, is it possible to label the ATP turnover of the molecular
motor (kinesin) driving vesicle movement in vivo?