""Jason R. Richardson"" <jrr1 at bully.net> wrote in message
news:000701bfcf2f$2d2b7ae0$78384ec6 at oemcomputer...
Does anyone know a good method for preparing nuclear extracts from rat
brain. I will be using western blotting to look at CREB and c-fos.
Sonicating the cells in a buffer containing protease inhibitors, then
centrifuging out the nuclei and cell membranes usually works. Any other
method that produces enough shearing will probably do ok, maybe repeatedly
aspirating through a real small gauge needle would work for small amounts if
you don't have a sonicator.
serotonin1 at hushmail dot com