Perforated Patch Problem

Matthew Ireland mfireland at yahoo.com
Mon Sep 3 22:27:23 EST 2001

Dear Colleagues 

Currently I am using perforated patch on neurons within the CNS.
Recently, I have been experinceing a problem when I try to perforate a
neuron, instead of a full perforation as you would expect, it only
results in a partial perforation.

I have tried different concentrations of Amphotericin B, different
filling times and modified electrodes. All without much success.

I would appreciate any suggestions

With thanks,

Matthew Ireland
University of Queensland

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