Dear Colleagues
Currently I am using perforated patch on neurons within the CNS.
Recently, I have been experinceing a problem when I try to perforate a
neuron, instead of a full perforation as you would expect, it only
results in a partial perforation.
I have tried different concentrations of Amphotericin B, different
filling times and modified electrodes. All without much success.
I would appreciate any suggestions
With thanks,
Matthew Ireland
University of Queensland
