Alhtough not likely, but could your ampho have gone nearly completely
gone bad?
On 3 Sep 2001 20:27:23 -0700, mfireland at yahoo.com (Matthew Ireland)
wrote:
>Dear Colleagues
>>Currently I am using perforated patch on neurons within the CNS.
>Recently, I have been experinceing a problem when I try to perforate a
>neuron, instead of a full perforation as you would expect, it only
>results in a partial perforation.
>>I have tried different concentrations of Amphotericin B, different
>filling times and modified electrodes. All without much success.
>>I would appreciate any suggestions
>>With thanks,
>>Matthew Ireland
>University of Queensland