On 3 Sep 2001, Matthew Ireland wrote:
> Dear Colleagues
> Currently I am using perforated patch on neurons within the CNS.
> Recently, I have been experinceing a problem when I try to perforate a
> neuron, instead of a full perforation as you would expect, it only
> results in a partial perforation.
Not sure what a partial perforation is. Are you in fact getting
a proper seal? I suppose the ampB could go bad, or it could
leak out of the pipette and permeabilize the cells as well.
Are you back filling a small volume of the ampB into a large
volume of ampB-free buffer in the pipette?