How about trying rat DRGs or anti VR1 & VR2 distinguish between small
and large neurons. I believe they are commercially available
celiacruz at netc.pt wrote:
> Hi everyone!
>> I am a portuguese Ph.D student and I'm trying
> to stain big and medium-sized DRG neurons with
> NF200 (N52) antibody using the ABC technique.
> The problem is that the antibody is made in
> mouse and I'm using mouse tissue. I have already
> bought the Mouse on Mouse Kit from Vector but
> I am still having problems. I first tryed diluting
> the antibody 1/500 and 1/1000. Since I got
> a lot of background, I tryed diluting it in
> PBST and incubating at 4ºC. Still bad. I've
> tryed blocking with glycine and normal goat
> serum. Worse!
>> I'm really running out of ideas. Can anyone
> give me a hint (as soon as possible, please)on
> how to proceed?
>> Many thanks to all,